|
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
|
Mycosanitea
Cosmic Clown



Registered: 01/26/13
Posts: 25
Loc: PNW
Last seen: 9 years, 23 days
|
re-pasteurizing contaminated substrate
#21591180 - 04/24/15 04:10 PM (9 years, 25 days ago) |
|
|
Last weekend, I pasteurized about 15 gallons of bulk sub. I was planning on using it the next day, after it cooled, but was unexpectedly delayed. Then I had to work all week and wasn't able to get back to my project until 6 days later. Now the sub has bacterial contamination and smells of rotten eggs. Is it OK to re-pasteurize and use, or should I throw it out and start over? I really don't want to throw it out, if I don't have to, as I am too broke to be wasteful.
-------------------- "Watching TV is like taking black spray paint to your third eye." -Bill Hicks
|
cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 3 months
|
Re: re-pasteurizing contaminated substrate [Re: Mycosanitea]
#21591192 - 04/24/15 04:13 PM (9 years, 25 days ago) |
|
|
what was your bulk sub? a good pasturizing should have bought ya two weeks not six days i would say your lucky you didn't use it if anything and i would start over....bulk is cheap
--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
|
Mycosanitea
Cosmic Clown



Registered: 01/26/13
Posts: 25
Loc: PNW
Last seen: 9 years, 23 days
|
Re: re-pasteurizing contaminated substrate [Re: cronicr]
#21591259 - 04/24/15 04:26 PM (9 years, 25 days ago) |
|
|
20% coir chunks (because the only verm source in town stopped carrying it) 20% coir pith 20% straw 20% steer manure 20% worm castings And gypsum = to 10% volume
I don't have a steam pasteurizer yet, so I just put it in a 10 gallon stock pot and simmer it for a few hours on the stove. Stirring every half hour or so until I'm content that all of it has been steamed sufficiently.
It's worked fine in the past, for lack of a better option, but I've always used it within a day or 2.
-------------------- "Watching TV is like taking black spray paint to your third eye." -Bill Hicks
|
cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 3 months
|
Re: re-pasteurizing contaminated substrate [Re: Mycosanitea]
#21591279 - 04/24/15 04:31 PM (9 years, 25 days ago) |
|
|
i would do a better pasturizing then that, bring your mix to field capacity and load it into jars, put them in a pot with cold water coming just over halfway up the jars and crank the heat on, once it starts to boil sht it off and monitor the core temp of the middle jar and make sure it stays within good range
--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
|
Mycosanitea
Cosmic Clown



Registered: 01/26/13
Posts: 25
Loc: PNW
Last seen: 9 years, 23 days
|
Re: re-pasteurizing contaminated substrate [Re: cronicr]
#21591295 - 04/24/15 04:34 PM (9 years, 25 days ago) |
|
|
15 gallons is a lot of jars. Garbage bag and a 55 gallon drum is more like what I need. Or the cooler tek.
-------------------- "Watching TV is like taking black spray paint to your third eye." -Bill Hicks
Edited by Mycosanitea (04/24/15 04:38 PM)
|
Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
|
Re: re-pasteurizing contaminated substrate [Re: Mycosanitea]
#21591311 - 04/24/15 04:38 PM (9 years, 25 days ago) |
|
|
You don't make 15 gallons of substrate in a 10 gallon pot :/.. I can fit 7 qts per pot so it's basically enough for 1 66 qt tub per 2 pots
It takes half an hour to get to temps and then 90 minutes of pasteurization. Makes it last 2-3 weeks without contamination unless you have it sitting in the draft of the fucking house vent >.>... still only lost 2 tubs so what ever.
Edited by Mad Season (04/24/15 04:40 PM)
|
blackdust


Registered: 02/28/09
Posts: 8,327
|
Re: re-pasteurizing contaminated substrate [Re: Mycosanitea]
#21591312 - 04/24/15 04:38 PM (9 years, 25 days ago) |
|
|
mushroom growing is a race
|
Mycosanitea
Cosmic Clown



Registered: 01/26/13
Posts: 25
Loc: PNW
Last seen: 9 years, 23 days
|
Re: re-pasteurizing contaminated substrate [Re: Mad Season]
#21591318 - 04/24/15 04:39 PM (9 years, 25 days ago) |
|
|
You might not make 15 gallons of substrate. I, on the other hand, do.
-------------------- "Watching TV is like taking black spray paint to your third eye." -Bill Hicks
|
blindingleaf
blue collar underworld


Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
|
Re: re-pasteurizing contaminated substrate [Re: Mycosanitea]
#21591323 - 04/24/15 04:40 PM (9 years, 25 days ago) |
|
|
use those oven bags for turkeys. cron is right, it should have lasted longer than 6 days. and if anything, it should have gotten mold i would have guessed. simmering it alone, w/o protection for jar or bag, is cooking it too high to be considered safe for pasteurization. can u put it in a pot, then put that pot into the larger one that has water at the bottom? even if u can't take temp (try to get a thermometer) that set up is safer than what ur doing. its good that it worked before, but is not the most reliable way.
i would get new substrate if it was me. just grab what u can, doesn't have to be all of those things every time. even just straw and castings. coir alone, etc.
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
|
Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
|
Re: re-pasteurizing contaminated substrate [Re: Mycosanitea]
#21591335 - 04/24/15 04:41 PM (9 years, 25 days ago) |
|
|
Quote:
Mycosanitea said: You might not make 15 gallons of substrate. I, on the other hand, do.
I edited it. I actually have 10 tubs and 20 trays going all from jars.. and they're 105 qt tubs that took 21 qts each. It's better to get it done right than just to get lucky once and have it fail when you have 15 gallons of substrate.
Edited by Mad Season (04/24/15 04:45 PM)
|
cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 3 months
|
Re: re-pasteurizing contaminated substrate [Re: Mad Season]
#21591357 - 04/24/15 04:46 PM (9 years, 25 days ago) |
|
|
if you are doing 15 gallons at a time for sure look into a proper pasturizing
--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
|
Mycosanitea
Cosmic Clown



Registered: 01/26/13
Posts: 25
Loc: PNW
Last seen: 9 years, 23 days
|
Re: re-pasteurizing contaminated substrate [Re: cronicr]
#21591381 - 04/24/15 04:50 PM (9 years, 25 days ago) |
|
|
Ok. That makes more sense. I split it in half. 7.5 gallons per pot. That way, I have room to stir. 2 hours per 7 quarts (1.75 gallons) with 15 minutes of processing time per batch means more than 20 hours of pasteurization. That's my entire weekend right there. Besides, I'm not looking for pasteurization teks. I just want to know if it is safe to re-pasteurize. I'm planning on building a steam pasteurizer eventually.
-------------------- "Watching TV is like taking black spray paint to your third eye." -Bill Hicks
Edited by Mycosanitea (04/24/15 04:58 PM)
|
Mad Season
hookers and blackjack



Registered: 09/16/12
Posts: 12,666
Loc: Canada
|
Re: re-pasteurizing contaminated substrate [Re: Mycosanitea]
#21591390 - 04/24/15 04:52 PM (9 years, 25 days ago) |
|
|
If it were mine I wouldn't
|
cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 3 months
|
Re: re-pasteurizing contaminated substrate [Re: Mycosanitea]
#21591401 - 04/24/15 04:53 PM (9 years, 25 days ago) |
|
|
well it may or may not be safe to do so...if you nuked all your benificial microbes which i suspect you did it may be useless
--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
|
blackdust


Registered: 02/28/09
Posts: 8,327
|
Re: re-pasteurizing contaminated substrate [Re: Mycosanitea]
#21591458 - 04/24/15 05:00 PM (9 years, 25 days ago) |
|
|
Quote:
Mycosanitea said: Ok. That makes more sense. I split it in half. 7.5 gallons per pot. That way, I have room to stir. 2 hours per 7 quarts (1.75 gallons) with 15 minutes of processing time per batch means more than 20 hours of pasteurization. That's my entire weekend right there. Besides, I'm not looking for pasteurization teks. I just want to know if it is safe to re-pasteurize. I'm planning on building a steam pasteurizer eventually.
\do it then overcompensate with extra clean spawn. I would want no more then a 5 day spawn run.
|
azur
God of Fuck



Registered: 04/21/12
Posts: 28,103
Loc: Daid
|
Re: re-pasteurizing contaminated substrate [Re: Mycosanitea]
#21591493 - 04/24/15 05:07 PM (9 years, 25 days ago) |
|
|
How did you fit 15 gallons in a 10 gallon pot?
Do like cron said, but use hot water. Idk why people say to start with cold water. It's more efficient to start with hot
|
cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 3 months
|
Re: re-pasteurizing contaminated substrate [Re: azur]
#21591504 - 04/24/15 05:09 PM (9 years, 25 days ago) |
|
|
brings the temps up more evenly
--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
|
azur
God of Fuck



Registered: 04/21/12
Posts: 28,103
Loc: Daid
|
Re: re-pasteurizing contaminated substrate [Re: cronicr]
#21591519 - 04/24/15 05:10 PM (9 years, 25 days ago) |
|
|
Fuck that. I use hot water to bring my sub to field capacity and i put hot water in my pots. I want to get it to temp as fast as possible. I do understand what you're saying though
|
cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 3 months
|
Re: re-pasteurizing contaminated substrate [Re: azur]
#21591527 - 04/24/15 05:12 PM (9 years, 25 days ago) |
|
|
i can get by with hot water as well but i know my stove so well that using everything the same gives me the same results and i dont' even need my meat thermometer anymore
--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
|
Mycosanitea
Cosmic Clown



Registered: 01/26/13
Posts: 25
Loc: PNW
Last seen: 9 years, 23 days
|
Re: re-pasteurizing contaminated substrate [Re: azur]
#21591549 - 04/24/15 05:16 PM (9 years, 25 days ago) |
|
|
Quote:
azur said: How did you fit 15 gallons in a 10 gallon pot?
Do like cron said, but use hot water. Idk why people say to start with cold water. It's more efficient to start with hot
Never said I put all 15 gallons in at once.
-------------------- "Watching TV is like taking black spray paint to your third eye." -Bill Hicks
|
|