Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: OlympusMyco.com Olympus Myco Bulk Substrate   MagicBag.co All-In-One Bags That Don't Suck   Left Coast Kratom Buy Kratom Extract   North Spore Bulk Substrate   Myyco.com Golden Teacher Liquid Culture For Sale   Original Sensible Seeds Bulk Cannabis Seeds

Jump to first unread post Pages: 1 | 2 | Next >  [ show all ]
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
OfflineMycosanitea
Cosmic Clown
Male


Registered: 01/26/13
Posts: 25
Loc: PNW
Last seen: 9 years, 23 days
re-pasteurizing contaminated substrate
    #21591180 - 04/24/15 04:10 PM (9 years, 25 days ago)

Last weekend, I pasteurized about 15 gallons of bulk sub. I was planning on using it the next day, after it cooled, but was unexpectedly delayed. Then I had to work all week and wasn't able to get back to my project until 6 days later. Now the sub has bacterial contamination and smells of rotten eggs. Is it OK to re-pasteurize and use, or should I throw it out and start over? I really don't want to throw it out, if I don't have to, as I am too broke to be wasteful.


--------------------
"Watching TV is like taking black spray paint to your third eye."
                                      -Bill Hicks

Extras: Filter Print Post Top
OfflinecronicrFacebook
 User Gallery


Registered: 08/07/11
Posts: 61,436
Loc: Van Isle Flag
Last seen: 2 years, 3 months
Trusted Cultivator
Re: re-pasteurizing contaminated substrate [Re: Mycosanitea]
    #21591192 - 04/24/15 04:13 PM (9 years, 25 days ago)

what was your bulk sub? a good pasturizing should have bought ya two weeks not six days:crazy: i would say your lucky you didn't use it if anything and i would start over....bulk is cheap


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor

Extras: Filter Print Post Top
OfflineMycosanitea
Cosmic Clown
Male


Registered: 01/26/13
Posts: 25
Loc: PNW
Last seen: 9 years, 23 days
Re: re-pasteurizing contaminated substrate [Re: cronicr]
    #21591259 - 04/24/15 04:26 PM (9 years, 25 days ago)

20% coir chunks (because the only verm source in town stopped carrying it)
20% coir pith
20% straw
20% steer manure
20% worm castings
And gypsum = to 10% volume

I don't have a steam pasteurizer yet, so I just put it in a 10 gallon stock pot and simmer it for a few hours on the stove. Stirring every half hour or so until I'm content that all of it has been steamed sufficiently.

It's worked fine in the past, for lack of a better option, but I've always used it within a day or 2.


--------------------
"Watching TV is like taking black spray paint to your third eye."
                                      -Bill Hicks

Extras: Filter Print Post Top
OfflinecronicrFacebook
 User Gallery


Registered: 08/07/11
Posts: 61,436
Loc: Van Isle Flag
Last seen: 2 years, 3 months
Trusted Cultivator
Re: re-pasteurizing contaminated substrate [Re: Mycosanitea]
    #21591279 - 04/24/15 04:31 PM (9 years, 25 days ago)

i would do a better pasturizing then that, bring your mix to field capacity and load it into jars, put them in a pot with cold water coming just over halfway up the jars and crank the heat on, once it starts to boil sht it off and monitor the core temp of the middle jar and make sure it stays within good range


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor

Extras: Filter Print Post Top
OfflineMycosanitea
Cosmic Clown
Male


Registered: 01/26/13
Posts: 25
Loc: PNW
Last seen: 9 years, 23 days
Re: re-pasteurizing contaminated substrate [Re: cronicr]
    #21591295 - 04/24/15 04:34 PM (9 years, 25 days ago)

15 gallons is a lot of jars. Garbage bag and a 55 gallon drum is more like what I need. Or the cooler tek.


--------------------
"Watching TV is like taking black spray paint to your third eye."
                                      -Bill Hicks

Edited by Mycosanitea (04/24/15 04:38 PM)

Extras: Filter Print Post Top
InvisibleMad Season
hookers and blackjack
 User Gallery


Registered: 09/16/12
Posts: 12,666
Loc: Canada Flag
Trusted Cultivator
Re: re-pasteurizing contaminated substrate [Re: Mycosanitea]
    #21591311 - 04/24/15 04:38 PM (9 years, 25 days ago)

You don't make 15 gallons of substrate in a 10 gallon pot :/.. I can fit 7 qts per pot so it's basically enough for 1 66 qt tub per 2 pots

It takes half an hour to get to temps and then  90 minutes of pasteurization. Makes it last 2-3 weeks without contamination unless you have it sitting in the draft of the fucking house vent >.>... still only lost 2 tubs so what ever.


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.

Edited by Mad Season (04/24/15 04:40 PM)

Extras: Filter Print Post Top
Invisibleblackdust
 User Gallery

Registered: 02/28/09
Posts: 8,327
Re: re-pasteurizing contaminated substrate [Re: Mycosanitea]
    #21591312 - 04/24/15 04:38 PM (9 years, 25 days ago)

mushroom growing is a race

Extras: Filter Print Post Top
OfflineMycosanitea
Cosmic Clown
Male


Registered: 01/26/13
Posts: 25
Loc: PNW
Last seen: 9 years, 23 days
Re: re-pasteurizing contaminated substrate [Re: Mad Season]
    #21591318 - 04/24/15 04:39 PM (9 years, 25 days ago)

You might not make 15 gallons of substrate. I, on the other hand, do.


--------------------
"Watching TV is like taking black spray paint to your third eye."
                                      -Bill Hicks

Extras: Filter Print Post Top
Invisibleblindingleaf
blue collar underworld
 User Gallery

Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
Trusted Cultivator
Re: re-pasteurizing contaminated substrate [Re: Mycosanitea]
    #21591323 - 04/24/15 04:40 PM (9 years, 25 days ago)

use those oven bags for turkeys.
cron is right, it should have lasted longer than 6 days.  and if anything, it should have gotten mold i would have guessed.
simmering it alone, w/o protection for jar or bag, is cooking it too high to be considered safe for pasteurization.  can u put it in a pot, then put that pot into the larger one that has water at the bottom?  even if u can't take temp (try to get a thermometer) that set up is safer than what ur doing.  its good that it worked before, but is not the most reliable way.

i would get new substrate if it was me.  just grab what u can, doesn't have to be all of those things every time.  even just straw and castings.  coir alone, etc.


--------------------
A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

The whole is greater than the sum of its parts

Extras: Filter Print Post Top
InvisibleMad Season
hookers and blackjack
 User Gallery


Registered: 09/16/12
Posts: 12,666
Loc: Canada Flag
Trusted Cultivator
Re: re-pasteurizing contaminated substrate [Re: Mycosanitea]
    #21591335 - 04/24/15 04:41 PM (9 years, 25 days ago)

Quote:

Mycosanitea said:
You might not make 15 gallons of substrate. I, on the other hand, do.



I edited it. I actually have 10 tubs and 20 trays going all from jars.. and they're 105 qt tubs that took 21 qts each. It's better to get it done right than just to get lucky once and have it fail when you have 15 gallons of substrate.


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.

Edited by Mad Season (04/24/15 04:45 PM)

Extras: Filter Print Post Top
OfflinecronicrFacebook
 User Gallery


Registered: 08/07/11
Posts: 61,436
Loc: Van Isle Flag
Last seen: 2 years, 3 months
Trusted Cultivator
Re: re-pasteurizing contaminated substrate [Re: Mad Season]
    #21591357 - 04/24/15 04:46 PM (9 years, 25 days ago)

if you are doing 15 gallons at a time for sure look into a proper pasturizing


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor

Extras: Filter Print Post Top
OfflineMycosanitea
Cosmic Clown
Male


Registered: 01/26/13
Posts: 25
Loc: PNW
Last seen: 9 years, 23 days
Re: re-pasteurizing contaminated substrate [Re: cronicr]
    #21591381 - 04/24/15 04:50 PM (9 years, 25 days ago)

Ok. That makes more sense. I split it in half. 7.5 gallons per pot. That way, I have room to stir. 2 hours per 7 quarts (1.75 gallons) with 15 minutes of processing time per batch means more than 20 hours of pasteurization. That's my entire weekend right there. Besides, I'm not looking for pasteurization teks. I just want to know if it is safe to re-pasteurize. I'm planning on building a steam pasteurizer eventually.


--------------------
"Watching TV is like taking black spray paint to your third eye."
                                      -Bill Hicks

Edited by Mycosanitea (04/24/15 04:58 PM)

Extras: Filter Print Post Top
InvisibleMad Season
hookers and blackjack
 User Gallery


Registered: 09/16/12
Posts: 12,666
Loc: Canada Flag
Trusted Cultivator
Re: re-pasteurizing contaminated substrate [Re: Mycosanitea]
    #21591390 - 04/24/15 04:52 PM (9 years, 25 days ago)

If it were mine I wouldn't


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.

Extras: Filter Print Post Top
OfflinecronicrFacebook
 User Gallery


Registered: 08/07/11
Posts: 61,436
Loc: Van Isle Flag
Last seen: 2 years, 3 months
Trusted Cultivator
Re: re-pasteurizing contaminated substrate [Re: Mycosanitea]
    #21591401 - 04/24/15 04:53 PM (9 years, 25 days ago)

well it may or may not be safe to do so...if you nuked all your benificial microbes which i suspect you did it may be useless:shrug:


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor

Extras: Filter Print Post Top
Invisibleblackdust
 User Gallery

Registered: 02/28/09
Posts: 8,327
Re: re-pasteurizing contaminated substrate [Re: Mycosanitea]
    #21591458 - 04/24/15 05:00 PM (9 years, 25 days ago)

Quote:

Mycosanitea said:
Ok. That makes more sense. I split it in half. 7.5 gallons per pot. That way, I have room to stir. 2 hours per 7 quarts (1.75 gallons) with 15 minutes of processing time per batch means more than 20 hours of pasteurization. That's my entire weekend right there. Besides, I'm not looking for pasteurization teks. I just want to know if it is safe to re-pasteurize. I'm planning on building a steam pasteurizer eventually.




\do it then overcompensate with extra clean spawn. I would want no more then a 5 day spawn run.

Extras: Filter Print Post Top
Invisibleazur
God of Fuck
Male User Gallery


Registered: 04/21/12
Posts: 28,103
Loc: Daid
Re: re-pasteurizing contaminated substrate [Re: Mycosanitea]
    #21591493 - 04/24/15 05:07 PM (9 years, 25 days ago)

How did you fit 15 gallons in a 10 gallon pot?

Do like cron said, but use hot water. Idk why people say to start with cold water. It's more efficient to start with hot


--------------------


A cube is NOT a cube.

FALL IN LOVE WITH LC
FOTTSE!!!
ALL NOOBS READ THIS!!!


Extras: Filter Print Post Top
OfflinecronicrFacebook
 User Gallery


Registered: 08/07/11
Posts: 61,436
Loc: Van Isle Flag
Last seen: 2 years, 3 months
Trusted Cultivator
Re: re-pasteurizing contaminated substrate [Re: azur]
    #21591504 - 04/24/15 05:09 PM (9 years, 25 days ago)

brings the temps up more evenly:thumbup:


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor

Extras: Filter Print Post Top
Invisibleazur
God of Fuck
Male User Gallery


Registered: 04/21/12
Posts: 28,103
Loc: Daid
Re: re-pasteurizing contaminated substrate [Re: cronicr]
    #21591519 - 04/24/15 05:10 PM (9 years, 25 days ago)

Fuck that. I use hot water to bring my sub to field capacity and i put hot water in my pots. I want to get it to temp as fast as possible. I do understand what you're saying though


--------------------


A cube is NOT a cube.

FALL IN LOVE WITH LC
FOTTSE!!!
ALL NOOBS READ THIS!!!


Extras: Filter Print Post Top
OfflinecronicrFacebook
 User Gallery


Registered: 08/07/11
Posts: 61,436
Loc: Van Isle Flag
Last seen: 2 years, 3 months
Trusted Cultivator
Re: re-pasteurizing contaminated substrate [Re: azur]
    #21591527 - 04/24/15 05:12 PM (9 years, 25 days ago)

i can get by with hot water as well but i know my stove so well that using everything the same gives me the same results and i dont' even need my meat thermometer anymore:lol:


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor

Extras: Filter Print Post Top
OfflineMycosanitea
Cosmic Clown
Male


Registered: 01/26/13
Posts: 25
Loc: PNW
Last seen: 9 years, 23 days
Re: re-pasteurizing contaminated substrate [Re: azur]
    #21591549 - 04/24/15 05:16 PM (9 years, 25 days ago)

Quote:

azur said:
How did you fit 15 gallons in a 10 gallon pot?

Do like cron said, but use hot water. Idk why people say to start with cold water. It's more efficient to start with hot





Never said I put all 15 gallons in at once.


--------------------
"Watching TV is like taking black spray paint to your third eye."
                                      -Bill Hicks

Extras: Filter Print Post Top
Jump to top Pages: 1 | 2 | Next >  [ show all ]

Shop: OlympusMyco.com Olympus Myco Bulk Substrate   MagicBag.co All-In-One Bags That Don't Suck   Left Coast Kratom Buy Kratom Extract   North Spore Bulk Substrate   Myyco.com Golden Teacher Liquid Culture For Sale   Original Sensible Seeds Bulk Cannabis Seeds


Similar ThreadsPosterViewsRepliesLast post
* Help! Not sure if contaminated... splasher78 921 3 08/12/02 09:02 AM
by kykeon
* Cased Substrate Water Reservoir? supairish 1,434 5 08/08/01 09:31 AM
by Shiznitz
* Re: The Dangers Of Eating From Contaminated Cakes
( 1 2 all )
Sclorch 75,401 23 07/03/00 01:42 PM
by Anonymous
* contaminated jar rejuvination... fig 2,019 4 03/01/02 10:45 PM
by MushyMay
* Pasteurize or sterilize? Skikid16 1,065 4 10/12/02 02:22 AM
by socratesmind
* cleaning after contamination TeRzMaStA 1,451 7 11/20/01 02:44 PM
by OLD_NEWBIE
* When would contamination be first visible? Fd3000 1,077 6 06/07/02 10:18 AM
by luvdemshrooms
* storing pasteurized poo.... sBUD 2,024 6 08/19/02 03:39 PM
by entheo_smurf

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
687 topic views. 22 members, 131 guests and 43 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.041 seconds spending 0.01 seconds on 16 queries.