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Offlinenihilist
another systemof control

Registered: 06/23/03
Posts: 169
Loc: USA
Last seen: 4 years, 10 months
positive or negative pressure box?
    #2156598 - 12/03/03 10:22 PM (13 years, 1 month ago)

I want to make a GOOD glove box.  I want to do either a positve pressure box with the fan blowing in from the filter, or negative pressure with a top-mounted fan blowing air out as it comes in through filters.

I just have questions I think you guys could help me out with:

1)  With the positive pressure box, where does the air escape?  Do you make exhaust holes?

2)  With the negative pressure idea, can i achieve a completely sterile airflow with 70 mm synthecic micron filter disks?  Possibly with a tyvek and coffee filter prefilter?  Or do I HAVE to have a HEPA to achieve this?      :confused: 


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InvisibleautomanM
blasted chipmunk
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Registered: 09/18/03
Posts: 7,911
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Re: positive or negative pressure box? [Re: nihilist]
    #2156635 - 12/03/03 10:33 PM (13 years, 1 month ago)

use a possitive pressure box. if you have a seal on the box, holes stuffed with polyfill will make nice outvents.
if there isnt a good seal, the air will find its own way out.


--------------------
No, no, you're not thinking, you're just being logical. ~ Niels Bohr


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Offlinenihilist
another systemof control

Registered: 06/23/03
Posts: 169
Loc: USA
Last seen: 4 years, 10 months
Re: positive or negative pressure box? [Re: automan]
    #2156694 - 12/03/03 10:54 PM (13 years, 1 month ago)

Thanks for the advice. I think I will do a positive pressure, if anything, so that I worry less about leaks. I don't know what polyfill is, but would micron filters let enough air pass through them to use them on my exhaust holes. I think I might be getting paranoid about contams getting back in through there somehow, but I want to be 100% sure I'm doing things right.


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Offlinenihilist
another systemof control

Registered: 06/23/03
Posts: 169
Loc: USA
Last seen: 4 years, 10 months
Re: positive or negative pressure box? [Re: nihilist]
    #2156710 - 12/03/03 11:00 PM (13 years, 1 month ago)

Oh, I shoudl probably explain what I'm planning on doing with the box. I don't want to have a bottom on it. I wanted to have a seal (weatherstrip?) on the bottom (which is the opening of the rubbermaid tub) so that it coulds attach to another rubbermaid, that I will be using as a fruiting chamber. It will have a plexiglass top with a port hole covered with plexi glass that would be bolted down onto a gasket arond the opening.

My idea is that I could, inside the glovebox, flame sterilize some of my equipment and then wipe everything down with alcohol and allow the filter to take in clean air afterwards. Then I would open the oprt, repeat that process, and then case.

Does this seem like a good iead? Any flaws you can see in that plan?


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Offlinefidget
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Registered: 03/18/02
Posts: 245
Loc: Oregon
Last seen: 11 years, 1 month
Re: positive or negative pressure box? [Re: nihilist]
    #2156743 - 12/03/03 11:10 PM (13 years, 1 month ago)

Here is the post of the one that i made. Seems to be working very well. I plan to test some agar out in it soon.

Positive Pressure Glove Box





--------------------
- fidget

"With ordinary consciousness you can't even begin to know what's happening."



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Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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