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OfflineLawnboy1185
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Frist grow, second attempt, no mycelium
    #21550089 - 04/15/15 07:52 AM (9 years, 1 month ago)

8 days with no mycelium growth at all.  Using verm/brf cakes, it looks like the jars have setteled since inoculation and there is a half inch of space where the vermiculite top layer used to be.  Temperature has been steady at around 72 degrees in the open air.


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Offlineel8tch
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Re: Frist grow, second attempt, no mycelium [Re: Lawnboy1185]
    #21550160 - 04/15/15 08:27 AM (9 years, 1 month ago)

Did you inoculate toward the outside of the jar?

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OfflineLawnboy1185
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Re: Frist grow, second attempt, no mycelium [Re: el8tch]
    #21550181 - 04/15/15 08:36 AM (9 years, 1 month ago)

I did, but I used the taller 1/2 pint jars...which I read afterwards were not good to use.  I have some 1/4 pint jars that I will inoculate today.  Ive already missed 2 full moons and an eclipse.  Frustrating


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Offlineel8tch
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Re: Frist grow, second attempt, no mycelium [Re: Lawnboy1185]
    #21550236 - 04/15/15 08:54 AM (9 years, 1 month ago)

Really? As long as it's a wide-mouth pint it will be fine. The only difference between them is the colonization times... People say that the short and fat half-pints colonize somewhat faster.

They might just be taking awhile... It does happen. I've read where some people haven't seen growth for around two weeks.. sometimes longer. I imagine the temps of the room the jars are in are decent? Somewhere between high 60s and high 70s?

Edited by el8tch (04/15/15 08:55 AM)

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OfflineIrdamage
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Re: Frist grow, second attempt, no mycelium [Re: el8tch]
    #21550263 - 04/15/15 09:03 AM (9 years, 1 month ago)

If you used an MS syringe it can take upwards of 2-3 weeks to see 1st growth. If you decide to move up to grains and bulk you can avoid that wait time by performing Grain 2 Grain transfers of myc that is already healthy.

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Invisiblejbaby007S
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Re: Frist grow, second attempt, no mycelium [Re: Irdamage]
    #21550332 - 04/15/15 09:31 AM (9 years, 1 month ago)

Did you use a syringe from a sponsor? I have tall half pints too and I've had no issue with them. Do you have pics? I don't think you should have space in the jar either. The dry verm layer should fill up the last half inch of the jar.


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InvisibleMad Season
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Re: Frist grow, second attempt, no mycelium [Re: jbaby007]
    #21551466 - 04/15/15 02:51 PM (9 years, 1 month ago)

Just give it time. Sounds like Temps could be a little low almost. If you're nervous and can't wait... JUST MAKE MORE! :P

For real tho some jars can take 5 weeks sometimes more. I've heard of people seeing no growth or contamination for 4+ weeks and they forgot about it in the back of the closet and checked 2 or 3 months later and it was almost done


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OfflinePsilicon
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Re: Frist grow, second attempt, no mycelium [Re: Mad Season]
    #21551572 - 04/15/15 03:16 PM (9 years, 1 month ago)

Quote:

Mad Season said:
Just give it time. Sounds like Temps could be a little low almost. If you're nervous and can't wait... JUST MAKE MORE! :P

For real tho some jars can take 5 weeks sometimes more. I've heard of people seeing no growth or contamination for 4+ weeks and they forgot about it in the back of the closet and checked 2 or 3 months later and it was almost done




I suspect there might be a bacteria problem with many of those.  But like everyone above, I agree that 8 days is no big deal to wait for germination.


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InvisibleMad Season
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Re: Frist grow, second attempt, no mycelium [Re: Psilicon]
    #21551609 - 04/15/15 03:23 PM (9 years, 1 month ago)

Well best thing to do and is what I do now is put the syringes onto agar. Then put it into jars. Only way to make it clean imo


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How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
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OfflineLawnboy1185
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Re: Frist grow, second attempt, no mycelium [Re: Mad Season]
    #21551931 - 04/15/15 04:34 PM (9 years, 1 month ago)

This stuff is so new to me.  Been enjoying fungi for 15 years or so, but mycology is something I am looking to take serious.

There is another 12 jars in the PC now.  My house temp is usually around 72, but I have all of the supplies for an incubator but everyone shot it down on the boards.

I may be graduating from PF tek before my first fruiting.  What is the best tek for most amount of fruits?


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OfflineLawnboy1185
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Re: Frist grow, second attempt, no mycelium [Re: Lawnboy1185]
    #21551948 - 04/15/15 04:39 PM (9 years, 1 month ago)

Where should I store the inoculated jars?


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InvisibleMad Season
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Re: Frist grow, second attempt, no mycelium [Re: Lawnboy1185]
    #21552006 - 04/15/15 04:51 PM (9 years, 1 month ago)

Temps fine. Just needs time


--------------------
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How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.

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OfflineLawnboy1185
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Re: Frist grow, second attempt, no mycelium [Re: Mad Season]
    #21552031 - 04/15/15 04:57 PM (9 years, 1 month ago)

Right now Ive been using a top shelf in my room, so the light comes in during the day time to some extent.  Is there a better place to store the next batch?


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OfflineFerather
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Re: Frist grow, second attempt, no mycelium [Re: Lawnboy1185]
    #21552040 - 04/15/15 05:00 PM (9 years, 1 month ago)

I am practicing using light in all stages for a grow, I've read light is used to convert some nutrition to a usable state.

Had a bag of rye that took 4 weeks to germinate from MS syringe, fully in the dark. Think it was old spores though.


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