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Offlineheadtrip
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Registered: 03/14/03
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%$@# the bacteria...
    #2154709 - 12/03/03 12:51 PM (13 years, 11 months ago)

just wanted to check if anybody has any solutions to prevent bacteria, afoaf cased some colonized wbs to 60/40 coir/verm and atleast half the casings he's done have turned foul with bacteria. I was think'n maybe he waited to long to case the wbs, the jars were fully colonized and he waited another week to 2 weeks. He does all his casings in front of the stove and microwaves the 60/40 the recommended time. Could he spray some disinfectant on the casings shortly after the bacteria is noticed or is it too late?


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InvisibleG a n j a
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Re: %$@# the bacteria... [Re: headtrip]
    #2154788 - 12/03/03 01:16 PM (13 years, 11 months ago)

Sounds like you need to sterilise the casing matterial for longer.
How open is the area were there becoming infected?


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Offlineheadtrip
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Re: %$@# the bacteria... [Re: G a n j a]
    #2154913 - 12/03/03 01:48 PM (13 years, 11 months ago)

casing material is put in the microwave for 5 minutes, stirred with sterile spoon, then microwaved for another 5 minutes. Not to sure what you mean by how open the area is? But the casing and substrate are put together in bread loaf tins in front of the oven, then tinfoil is put on them and they go in to an incubator for 3-5 days.


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Offlineenimatpyrt
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Re: %$@# the bacteria... [Re: headtrip]
    #2155168 - 12/03/03 02:42 PM (13 years, 11 months ago)

Nuke the casing material after you have mixed it up with a fork. Then boil some water and put the fork in it for 10-15 minutes. Remove from the water and wrap with clean cloth. Remove the casing material, stir again, nuke again, boil fork again, apply casing layer. Cross contamination is a big problem.


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Offlineheadtrip
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Re: %$@# the bacteria... [Re: enimatpyrt]
    #2155392 - 12/03/03 03:41 PM (13 years, 11 months ago)

what about rubbing alcohol? can he use that as a substitute for boiling water?


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InvisibleG a n j a
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Re: %$@# the bacteria... [Re: headtrip]
    #2155549 - 12/03/03 04:27 PM (13 years, 11 months ago)

Well, that sounds fine so far.
Was there any smell to the jars before you cased them?
Just trying to find were they gained entry.


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Offlineheadtrip
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Re: %$@# the bacteria... [Re: G a n j a]
    #2155650 - 12/03/03 04:55 PM (13 years, 11 months ago)

no smell before they were cased...is bacteria an airborne contam like trich or does it need to be transfered by something? oh and i just thought of something, one of the jars he used the grain in the bottom of the jar wasn't completely colonized there was a few grains that got mixed in.


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Edited by headtrip (12/03/03 04:57 PM)


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InvisibleG a n j a
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Re: %$@# the bacteria... [Re: headtrip]
    #2157303 - 12/04/03 03:09 AM (13 years, 11 months ago)

well, it sounds like the bacteria was in the jars (or jar)
before casing, the bottoms of jars can somtimes get too damp,
and this is were you can get bacterias forming. The jar you had
that wasnt fully colonised sounds like the culprite :frown:


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Offlineheadtrip
&%$#@!!

Registered: 03/14/03
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Re: %$@# the bacteria... [Re: G a n j a]
    #2164902 - 12/06/03 07:58 PM (13 years, 11 months ago)

damn that jar


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InvisibleJoshua
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Re: %$@# the bacteria... [Re: headtrip]
    #2164984 - 12/06/03 08:56 PM (13 years, 11 months ago)

After preparation, spray the surface of the substrate with a 1.5% sol'n of peroxide, then spray the casing layer with the same solution.

You may also try increasing the time you keep your casing mix in the microwave. I usually microwave Qt jars for 10 min, 3 times while waiting 20 min or longer in between. Overall, you need to maintain at least 165-170 F for 45-60 minutes to obtain pasteurization.

You said you, "cased some colonized wbs to 60/40 coir/verm."

Can you explain what exactly you did? Usually WBS is cased "with" a casing mix, or spawned "to or into" a bulk substrate. Which is it that you did?

Joshua


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Amazon Shop: ½ Pint Jars, Coir, Vermiculite, Wild Bird Seed

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