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Shop: PhytoExtractum Maeng Da Thai Kratom Leaf Powder   North Spore Injection Grain Bag

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OfflineClever_You
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agar sterilization
    #21546446 - 04/14/15 01:31 PM (9 years, 1 month ago)

serching threw the archives im getting different answers...how long is recomended for sterilizing my agar  20 min or 40 min

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InvisibleFantastic Mr. Fox
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Re: agar sterilization [Re: Clever_You]
    #21546485 - 04/14/15 01:43 PM (9 years, 1 month ago)

30min at 15psi.


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Giving is all we have, for we're just grateful to be alive

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InvisibleImperfect Iam
^means imperfect,not I'm perfect
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Re: agar sterilization [Re: Fantastic Mr. Fox]
    #21546628 - 04/14/15 02:25 PM (9 years, 1 month ago)

I always do mine for 40 min. at 15psi, but try thirty if u want, tell us how it goes


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All you touch, and all you see, is all your life will ever be- Pink Floyd

Life is what happens while you're busy making other plans- John Lennon

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Offlineblojo02184
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Re: agar sterilization [Re: Imperfect Iam]
    #21546671 - 04/14/15 02:33 PM (9 years, 1 month ago)

15 or 20 is acceptable if your doing smaller jars.

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InvisibleFantastic Mr. Fox
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Re: agar sterilization [Re: blojo02184]
    #21546686 - 04/14/15 02:35 PM (9 years, 1 month ago)

30min is sufficient.
40min wouldn't be over kill tho...
:thumbup:


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Giving is all we have, for we're just grateful to be alive

:kratom:

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InvisiblebodhisattaMDiscordReddit
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Re: agar sterilization [Re: Fantastic Mr. Fox]
    #21546763 - 04/14/15 02:48 PM (9 years, 1 month ago)

10-15m at 15psi is the standard for sterilization of agar mediums

right off a YPD agar bottle



I've never had a problem with plates because of insufficient sterilization ever. If I have the PC packed full I'll do 20m.

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InvisibleImperfect Iam
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Re: agar sterilization [Re: bodhisatta]
    #21546830 - 04/14/15 03:02 PM (9 years, 1 month ago)

Good to know Bodhi, u just saved me 20 minutes of pc time, thanks man!


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All you touch, and all you see, is all your life will ever be- Pink Floyd

Life is what happens while you're busy making other plans- John Lennon

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InvisibleFantastic Mr. Fox
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Re: agar sterilization [Re: Imperfect Iam]
    #21546866 - 04/14/15 03:09 PM (9 years, 1 month ago)

Im sayin' though. :lol:


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Giving is all we have, for we're just grateful to be alive

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InvisiblebodhisattaMDiscordReddit
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Re: agar sterilization [Re: Imperfect Iam]
    #21546877 - 04/14/15 03:11 PM (9 years, 1 month ago)

I've had no problem with telephone agar and at home (non lab grade) nutrient sources either. those are sterilized just as well as 100 dollar bottles of lab grade powdered pre mix agar, in a 15m 15psi cycles.

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OfflinecronicrFacebook
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Re: agar sterilization [Re: Clever_You]
    #21546996 - 04/14/15 03:40 PM (9 years, 1 month ago)

Quote:

Clever_You said:
serching threw the archives im getting different answers



stay out of the archives....it's archived for a reason lol


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