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Thoky.ykoht

Registered: 10/08/13
Posts: 399
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Re: help me with rye questions [Re: Thoky.ykoht]
#21561894 - 04/18/15 12:18 AM (9 years, 8 months ago) |
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Is anyone up? I did these last night and let them cool over night in my PC, I just transferred them to my SAB but the condensation in some of them it is heavy. Another note was water from my PC leaked into a couple plates. I didn't use them just thought I'd share how they looked haha kind of like a help cup. Can I transfer my agar to agar plates with condensation in them or should I wait? I gently have them during upside down in my box. Should I leave the paper them on these or ditch them?
Edited by Thoky.ykoht (04/18/15 12:20 AM)
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Buck513

Registered: 04/17/14
Posts: 5,682
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Re: help me with rye questions [Re: Thoky.ykoht]
#21561979 - 04/18/15 12:52 AM (9 years, 8 months ago) |
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I'm still trying to figure out why you have your plates upside down.
Why is the agar all along the side of that one plate? I deffinetly wouldn't use that, you don't need much.
As far as the condensation, no big deal.
-------------------- Fail to plan and you plan to fail.
Enter the Ban Lottery
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Thoky.ykoht

Registered: 10/08/13
Posts: 399
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Re: help me with rye questions [Re: Buck513]
#21562176 - 04/18/15 03:01 AM (9 years, 8 months ago) |
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I just read people leave them upside down. That one with the shit on the side is where water seeped in during their time in the PC causing the agar mixture to increase. Of course I know you don't need that much and I didn't use it. I'm just now seeing the response.
I went ahead and transferred to 13 plates, (water, agar, light malt extract)
 I had a sterile jar of rye in the cooker so I transferred to that as well
 And here's some visible growth from my drop 2 days ago. (zoom)
 Here's what's left of the plate I was using
 I plan on getting more jars and rye in the morning I have another plate that's almost reached its edges so I wanted to transfer it before then. I guess all I can do now is wait.
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professorFATTYCAP
Training 4 the mycothalon



Registered: 04/08/14
Posts: 751
Last seen: 1 month, 23 days
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Re: help me with rye questions [Re: Thoky.ykoht]
#21562426 - 04/18/15 06:53 AM (9 years, 8 months ago) |
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Why is the myc side of ur wedge in the jar in the center facing the glass instead of toward the grain
Edited by professorFATTYCAP (04/18/15 06:54 AM)
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,916
Loc: Milky way
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Quote:
professorFATTYCAP said: Why is the myc side of ur wedge in the jar in the center facing the glass instead of toward the grain
Sometimes you don't choose how it falls in, but it hardly matters
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Thoky.ykoht

Registered: 10/08/13
Posts: 399
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Re: help me with rye questions [Re: bodhisatta]
#21562646 - 04/18/15 09:00 AM (9 years, 8 months ago) |
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What he said ^^ even though the myc is facing the glass you can Cleary see the myc extending out on to the grain. I have pieces of agar I've dropped and the myc completely took over the other side of the agar while growing out into the rye. So it really is irrelevant if it is facing the glass atm. Plus they'll all get a good shake at 30% which then it really won't matter where the agar ends up.
Edited by Thoky.ykoht (04/18/15 09:15 AM)
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,916
Loc: Milky way
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Re: help me with rye questions [Re: Thoky.ykoht]
#21562984 - 04/18/15 10:45 AM (9 years, 8 months ago) |
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Nice work so far
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Thoky.ykoht

Registered: 10/08/13
Posts: 399
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Re: help me with rye questions [Re: bodhisatta]
#21563244 - 04/18/15 11:27 AM (9 years, 8 months ago) |
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Hey thanks man, I just put another 5lbs of rye to soak. Those burnt rye jars I sat aside and knocked up with those supposedly spent syringes still have yet to show any contam or growth, period. So hopefully that's a good thing.
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Thoky.ykoht

Registered: 10/08/13
Posts: 399
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Re: help me with rye questions [Re: Thoky.ykoht]
#21575557 - 04/21/15 08:26 AM (9 years, 8 months ago) |
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Here's an update: Jars from 4/15
 Jar from 4/18 is starting to grow out:
 Finally some success with agar, these were transferred 4/18 No contams so far
 Today I'll be transferring a grown out plate to rye and making up some more agar.
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Thoky.ykoht

Registered: 10/08/13
Posts: 399
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Re: help me with rye questions [Re: Thoky.ykoht]
#21577257 - 04/21/15 05:12 PM (9 years, 8 months ago) |
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Just finished these, saved a piece to transfer.
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professorFATTYCAP
Training 4 the mycothalon



Registered: 04/08/14
Posts: 751
Last seen: 1 month, 23 days
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Re: help me with rye questions [Re: bodhisatta]
#21577857 - 04/21/15 07:30 PM (9 years, 8 months ago) |
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Quote:
bodhisatta said:
Quote:
professorFATTYCAP said: Why is the myc side of ur wedge in the jar in the center facing the glass instead of toward the grain
Sometimes you don't choose how it falls in, but it hardly matters
Yeah it happens. But when it happens to you. Do u or do u not try to reposition it . The truth mind you. We r on the honor system here
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Thoky.ykoht

Registered: 10/08/13
Posts: 399
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I currently have 17 knocked up jars, of which were inoculated on separate dates. I also have 12 "agar/light malt extract/water" pasty plates. I have a chunk of agar from the last rye transfer that I'm hoping to transfer to more plates this evening. I went away for six days and wants to start by asking if these 6 jars of rye I pc'ed the day I left are still sterile and useable? I never removed the foil and theve just been sitting out with the rest of the jars.
 Here's everything else 8days later.... Jars from 4/15 Before I shook
 Some nice rhizo
 Myc ball of rye while shaking
 After shaking
 Jar from 4/18, considering shaking it. What do you think?
 Jars from 4/21
 Here's a couple plates, didn't photograph all of them. But here's what going on
 As an experiment/ accident I stumbled on to these, I had knock med some of the agar that I made after transferring agar shards. The piece I dropped on each of them had been knock to the side, I then flicked the side if the plate over and over moving the agar transfer around the fresh agar here's what came of them. Let me remind you all these were made on 4/18.
Edited by Thoky.ykoht (04/29/15 04:06 PM)
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Thoky.ykoht

Registered: 10/08/13
Posts: 399
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Re: help me with rye questions [Re: Thoky.ykoht]
#21616505 - 04/30/15 07:03 AM (9 years, 8 months ago) |
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Are six day old already pced rye jars safe to inoculate or what?
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Buck513

Registered: 04/17/14
Posts: 5,682
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Re: help me with rye questions [Re: Thoky.ykoht]
#21616547 - 04/30/15 07:20 AM (9 years, 8 months ago) |
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Properly prepared grain jars can be good for weeks after sterilizing.
Longest I've personally waited was like 12 days or so, and they were fine. I'm sure they would have been fine if I let them go longer also. 6days is nothing to worry about.
-------------------- Fail to plan and you plan to fail.
Enter the Ban Lottery
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Thoky.ykoht

Registered: 10/08/13
Posts: 399
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Re: help me with rye questions [Re: Buck513]
#21616596 - 04/30/15 07:42 AM (9 years, 8 months ago) |
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Score, thanks.
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blindingleaf
blue collar underworld


Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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yea, i prep grains ahead of time and store them for a week or two before using in general. best to use ASAP, but time constraints and what not make it hard to prep>PC>use. as far as the burnt grains, its not ideal, but i've had bags melt in PC after 3 hours, then just re PCd the same grains in new bags for another 1.5 hours. still works just fine. i would shake the jar from 4/18, but some people wait longer. i shake when growth is about 3" circle, and then again probably a week later. some people have strict views on shaking times, but in reality, if ur grain is sterilized well, and ur myc is healthy, its all good.
Quote:
professorFATTYCAP said:
Quote:
bodhisatta said:
Quote:
professorFATTYCAP said: Why is the myc side of ur wedge in the jar in the center facing the glass instead of toward the grain
Sometimes you don't choose how it falls in, but it hardly matters
Yeah it happens. But when it happens to you. Do u or do u not try to reposition it . The truth mind you. We r on the honor system here
i would not try to reposition it. it can work, but its risky. leap off will just be a little slower.
-------------------- A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!
The whole is greater than the sum of its parts
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Thoky.ykoht

Registered: 10/08/13
Posts: 399
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Wondering if I should shake these today. They were knocked up 4/21, it's been almost 2 weeks. I think it's a little slower because I used a little more coffee when soaking then I did with the previous batch. Only because I was soaking more rye.

Here's the ones from 4/15 I shook, recovering nicely.
 And the jar from 4/18 that I shook the next day. Starting to recover.
 I plan to knock up 6 more jars tonight I got more rye in the soaker so I can prep another 5 for tomorrow.
What's the shelf life on agar, or the rules on it touching the walls?
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,916
Loc: Milky way
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Re: help me with rye questions [Re: Thoky.ykoht]
#21626093 - 05/02/15 01:29 PM (9 years, 8 months ago) |
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I would wait on the shake. agar lasts two weeks longer than you think it did. The fridge helps to keep em better longer too up to 3-6months in there.
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Thoky.ykoht

Registered: 10/08/13
Posts: 399
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Re: help me with rye questions [Re: bodhisatta]
#21626790 - 05/02/15 05:10 PM (9 years, 8 months ago) |
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Do I need to take any precautions when storing in the fridge? Maybe throw them in a zip lock or put paper tape along the rim?
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blindingleaf
blue collar underworld


Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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Re: help me with rye questions [Re: Thoky.ykoht]
#21627152 - 05/02/15 06:52 PM (9 years, 8 months ago) |
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ur jar lids look so shiny, i remember when mine looked like that 
plates in zip locks for fridge are always a good idea IMO.
the last pic u posted...is that the original spore swipe plate? it looks pretty crazy
-------------------- A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!
The whole is greater than the sum of its parts
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