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Invisiblegrownright
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Registered: 10/06/14
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Looking for tips on monotub
    #21543011 - 04/13/15 05:35 PM (9 years, 1 month ago)

Hey everyone, so this is my third time doing a monotub. My first one was successful with a yield of 4.5 ounces dry (kinda proud for it being my first bulk and second overall grow) My second attempt I used too much water in the substrate and I guess a bacteria buildup occurred because a section of the monotub not only didnt colonize but also it had a very large black spot on it...

Anyways this go around Im trying to increase my yield a little, My setup is a 66qt sterilite container with a substrate depth of 3" with a spawn ratio of about 1:2 (a little more sub than spawn). The substrate is hpoo/cpoo/coir/verm/gypsum.


I kept the tape on the monotub until I saw the two big pins growing in the middle then replaced it with polyfil, tight on the bottom, loose on top. However my pinset isn't ideal, I don't have time during the day to fan/mist my tub, so I keep a box fan on on the other side of the room pointed towards my tub, my tub is on a shelf unit that's above the height of the fan, so the fans current isnt directly hitting the tub. The picture I posted looks worse than the pinset does in person, but that's cause of my shitty camera, haha. What should I do to increase the number of pins growing on the substrate? take the polyfil out of the top holes for sometime each day and then replace them as necessary to prevent over-drying?

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Offlinemushpunx
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Re: Looking for tips on monotub [Re: grownright]
    #21543102 - 04/13/15 05:59 PM (9 years, 1 month ago)

Point the fan at a wall in the same room as the tubs, not the tub

It takes a little while but learning to dial in the poly just right is the best thing you can do is for a good pinset IME. You want max FAE but dont want any dry spots. A layer of water droplets on the surface that will be constantly evaporating to trigger pins.

A balance between max FAE and max humidity. Listen to the substrate. Too many dry spots are an indicator to tighten up the poly a little.



This is a photo of how I like my tubs dialed in, there will be a little dry spot around the poly but most of the surface looks like this.

Other things you can do are try casing, definitly get into agar, it will be a great tool in making clean spawn but you should also get into cloning!


--------------------

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Edited by mushpunx (04/13/15 06:02 PM)

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OfflineSockadin
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Re: Looking for tips on monotub [Re: mushpunx]
    #21543198 - 04/13/15 06:21 PM (9 years, 1 month ago)

You already have a bacterial contamination in your substrate.

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Offlinemushpunx
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Re: Looking for tips on monotub [Re: Sockadin]
    #21543283 - 04/13/15 06:26 PM (9 years, 1 month ago)

Quote:

Tetramine said:
You already have a bacterial contamination in your substrate.




It does have that cottage cheese look doesnt it?


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InvisibleMad Season
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Re: Looking for tips on monotub [Re: mushpunx]
    #21543706 - 04/13/15 07:33 PM (9 years, 1 month ago)

Yep bacteria. I'd also just give it time. Maybe it'll have an amazing pinset. As for dialing in the polyfill make the bottom just tight enough for you not to be able to stick your finger in between it and the wall. Make the top have about 1-2 inches of clear plastic with no condensation around the holes. But I live in a dry climate so I need the humidity in there.

Also have the fan pointing at the wall away from it. Just needs to create airflow in the room.


--------------------
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Edited by Mad Season (04/13/15 07:34 PM)

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Invisiblegrownright
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Registered: 10/06/14
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Re: Looking for tips on monotub [Re: mushpunx]
    #21543906 - 04/13/15 08:18 PM (9 years, 1 month ago)

Quote:

mushpunx said:
Point the fan at a wall in the same room as the tubs, not the tub

It takes a little while but learning to dial in the poly just right is the best thing you can do is for a good pinset IME. You want max FAE but dont want any dry spots. A layer of water droplets on the surface that will be constantly evaporating to trigger pins.

A balance between max FAE and max humidity. Listen to the substrate. Too many dry spots are an indicator to tighten up the poly a little.



This is a photo of how I like my tubs dialed in, there will be a little dry spot around the poly but most of the surface looks like this.

Other things you can do are try casing, definitly get into agar, it will be a great tool in making clean spawn but you should also get into cloning!



Okay cool, Ive tightened my polyfil in the top holes slightly. There is a layer of condensation on the substrate, but there were way more dry spots than like in your picture.

Quote:

mushpunx said:
Quote:

Tetramine said:
You already have a bacterial contamination in your substrate.




It does have that cottage cheese look doesnt it?




Any idea of how it got contaminated? The spawn was clean with no contams. he substrate was cooked in an oven roaster until the core reached 160 degrees and then cooked for an hour and cooled overnight.


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InvisibleMad Season
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Re: Looking for tips on monotub [Re: grownright]
    #21543939 - 04/13/15 08:24 PM (9 years, 1 month ago)

Spawn can also have hidden bacteria. It's the reason I don't and never will do grain to grain. Agar is the best way to get clean spawn. At least I've found that.


--------------------
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How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
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InvisibleNumeroEno
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Re: Looking for tips on monotub [Re: Mad Season]
    #21543952 - 04/13/15 08:26 PM (9 years, 1 month ago)

You're always gonna have some bacteria. Bacillus is a bitch. All you can do is give the tub conditions that favor mycelial growth over bacterial growth.

Quote:

Mad Season said:
Spawn can also have hidden bacteria. It's the reason I don't and never will do grain to grain. Agar is the best way to get clean spawn. At least I've found that.




g2g is fine. I did g2g a bunch of bacteria once, but that was because there was excess water in the master.


--------------------

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OfflineOrganic_Magic
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Re: Looking for tips on monotub [Re: Mad Season]
    #21543971 - 04/13/15 08:29 PM (9 years, 1 month ago)

Yeah I only do g2g if its from a grain jar I inoculated with a for sure clean agar wedge. Even then you can still have bacteria hiding away in grain jars. Nothing is ever 100% sterile.


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Invisiblegrownright
Duke of Lizzards
I'm a teapot


Registered: 10/06/14
Posts: 1,030
Loc: Deep South Flag
Re: Looking for tips on monotub [Re: Mad Season]
    #21544054 - 04/13/15 08:45 PM (9 years, 1 month ago)

Definitely gonna have to try casing my monotubs, I feel Ill probably be able to control the humidity better with a casing

Quote:

Mad Season said:
Spawn can also have hidden bacteria. It's the reason I don't and never will do grain to grain. Agar is the best way to get clean spawn. At least I've found that.




Im really gonna have to get into agar... Does it require a flow hood, or could you do agar work in a SAB?


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InvisibleNumeroEno
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Re: Looking for tips on monotub [Re: Organic_Magic]
    #21544060 - 04/13/15 08:47 PM (9 years, 1 month ago)

Quote:

Organic_Magic said:
Yeah I only do g2g if its from a grain jar I inoculated with a for sure clean agar wedge. Even then you can still have bacteria hiding away in grain jars. Nothing is ever 100% sterile.




:whathesaid:


--------------------

:gd_icon:  Let it grow! Let it grow! Greatly yield!  :gd_icon:
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in

DOG FOOD AGAR

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Offlinemushpunx
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Re: Looking for tips on monotub [Re: NumeroEno]
    #21544364 - 04/13/15 10:07 PM (9 years, 1 month ago)

"Any idea of how it got contaminated? The spawn was clean with no contams. he substrate was cooked in an oven roaster until the core reached 160 degrees and then cooked for an hour and cooled overnight."


If you have bacteria chances are the spawn was not clean. Many times spawn wil not show any cobtams or smell even after G2G, and spawning and colonization untill you go to fruit your tub.

The only real way to guarentee clean spawn is to inoculate with a clean culture (usually on agar) and use immpecable sterile tek


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Invisibleazur
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Re: Looking for tips on monotub [Re: mushpunx]
    #21544417 - 04/13/15 10:24 PM (9 years, 1 month ago)

Why do you guys say that tub has bacteria?
I mean, every tub does have some bacteria. But that tub looks fine to me


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InvisibleNumeroEno
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Re: Looking for tips on monotub [Re: azur]
    #21544430 - 04/13/15 10:29 PM (9 years, 1 month ago)

Quote:

azur said:
Why do you guys say that tub has bacteria?
I mean, every tub does have some bacteria. But that tub looks fine to me




Yeah I didn't see anything obvious either.


--------------------

:gd_icon:  Let it grow! Let it grow! Greatly yield!  :gd_icon:
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in

DOG FOOD AGAR

MY ELECTRIC INOCULATION LOOP

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Invisibleazur
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Re: Looking for tips on monotub [Re: NumeroEno]
    #21544435 - 04/13/15 10:30 PM (9 years, 1 month ago)

Just a little myc piss. I always have that


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A cube is NOT a cube.

FALL IN LOVE WITH LC
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InvisibleMad Season
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Re: Looking for tips on monotub [Re: azur]
    #21544574 - 04/13/15 11:21 PM (9 years, 1 month ago)

The first pic just looks off to me. 2nd looks better except the black uncolonized splotches in the BG

Don't see why it wouldn't be a bad pinset though. Give it 72 hours op and it might be a better pinset


--------------------
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How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.

Edited by Mad Season (04/13/15 11:23 PM)

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Invisibleazur
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Re: Looking for tips on monotub [Re: Mad Season]
    #21544589 - 04/13/15 11:28 PM (9 years, 1 month ago)

Um...op only has one pic.
Consolidate if you want, but they fruit when they are ready. And that's real talk


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A cube is NOT a cube.

FALL IN LOVE WITH LC
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Invisibleblindingleaf
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Re: Looking for tips on monotub [Re: Mad Season]
    #21544961 - 04/14/15 02:55 AM (9 years, 1 month ago)

Quote:

Mad Season said:
Spawn can also have hidden bacteria. It's the reason I don't and never will do grain to grain. Agar is the best way to get clean spawn. At least I've found that.




i use to get mold a lot when doing g2g in the small ass SAB i had.
so i had to switch to using ALL wedge>jars.
try switching to pints for ur masters, and cook with qts.
the smaller volume of grain will be sterilized way better, and its easier to g2g from small container to large qt jars in SAB.
bacteria won't come from g2g (its RARE).  it will come from insufficient sterilization in PC or the innoculant.  if ur using agar, and ur sure its clean, i'd bet on PC times or high temp during colonization.


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InvisibleTheEaglesGift
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Re: Looking for tips on monotub [Re: blindingleaf]
    #21544997 - 04/14/15 03:19 AM (9 years, 1 month ago)

When I used WBS often I absolutely had to PC for at least 120 minutes or I had bacteria issues. Maybe my WBS was very dirty, because I know plenty get away with shorter times. Even now, working with cleaner oats, I still PC for 120 just to be safe. I see no reason at all not too. The added piece of mind and security is worth it.

OP, I'm with Azur. Your tubs don't look bacteria ridden.

Give them good 12/12 light cycle of strong 6500k spectrum, get a fan going in the room, and dial in your polyfil. If the sub is beaded with moisture, you can fan or loosen the poly. If the substrate is drying and lacks beaded moisture, you can mist lightly to replenish it.

Don't do anything too cray or drastic to that tub without running it by all of us first. Just be patient, that thing could definitely still pull through with some killer flushes. Sometimes tubs just act weird and are slow to flush.

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InvisibleMad Season
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Re: Looking for tips on monotub [Re: TheEaglesGift]
    #21545312 - 04/14/15 07:33 AM (9 years, 1 month ago)

Quote:

TheEaglesGift said:
When I used WBS often I absolutely had to PC for at least 120 minutes or I had bacteria issues. Maybe my WBS was very dirty, because I know plenty get away with shorter times. Even now, working with cleaner oats, I still PC for 120 just to be safe. I see no reason at all not too. The added piece of mind and security is worth it.




I have been doing 90 minutes but seriously almost all my g2gs from agar failed whereas very few of my agar to grains have. I'm not confident doing g2gs until I've got a flow hood cause eventually bacteria somehow gets to it in my sab. All my agars been contam free.. maybe it is dirty spawn.. I'll have to change my pc to 120 minutes cause now I'm paranoid. Wouldn't hurt anything to increase time.

If I drop 3 or so wedges per jar I can easily see it colonized in 2 weeks so it's not that slow compared to g2g. I just love the security of a2g

Edited by Mad Season (04/14/15 07:37 AM)

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