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Invisibleblackdust
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Registered: 02/28/09
Posts: 8,327
Grow Log - GWLC - WBS - Popcorn - Coir
    #21537459 - 04/12/15 01:50 PM (9 years, 1 month ago)

Hello,
    Thought I would share the start of a bulk grow and the chase of an isolate. Here is how it began:

  • Multispore syringe to agar
  • 3 transfers made of the fastest, cleanest, most rizo looking sections
  • By only doing a few transfers I was able to have the best growing mycelium while still leaving hundreds of varieties to find a clone
  • A total of 5 agar dishes were used in inoculating all masters
  • agar was made with agar powder, potato flakes, and green food coloring


Here is a 3rd plate after 11 days


  • 8 master jars were inoculated with the agar wedges
  • Grain use was WBS
  • Steep the grains for 4 hours and 2 hours drying (did not need to steep this long)
  • pickling lime and gypsum were added to the steep and lightly mixed in with a spoon without disturbing the grains
  • Pressure cooked for 120 min at 15 PSI
  • Inoculated on 3/26


Here are the jars on 3/26


  • Temps are 72 - 80F
  • light is 24 hours (to lazy to turn on and off lights)
  • Shaken at 5% colonization 3/30


Here are the jars, 2 days after shacking 4/2


Jars on 4/3


Jars on 4/5


Jars on 4/7


  • An GWLC was also inoculated on 3/26
  • Used 30% WBS grain water (from the prepared WBS grains) to 70% water
  • agar wedge was shaken into the LC
  • The sediment on the bottom is carmalization of the carbohydrates in the grain water and aid to the nutrition of the LC
  • Here is the LC, while shacking the wedge into the mix


Here is the GWLC on 3/26


Here is the GWLC on 4/10



What will happen next?

  • GWLC will be expanded to more LC using SHIPS and syringe filters
  • Popcorn Bags will be created for spawn
  • Several monos, tubs, and trays will be made
  • Oven pasteurized, coir subs
  • a mushroom cluster will be found and cloned for further isolating and testing
  • a good isolate will be stored in a slant



Hope you enjoy,
Blackdust :heart:

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OfflinecronicrFacebook
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Registered: 08/07/11
Posts: 61,436
Loc: Van Isle Flag
Last seen: 2 years, 3 months
Trusted Cultivator
Re: Grow Log - GWLC - WBS - Popcorn - Coir [Re: blackdust]
    #21537465 - 04/12/15 01:52 PM (9 years, 1 month ago)

:hi:


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor

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Invisibleblackdust
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Registered: 02/28/09
Posts: 8,327
Re: Grow Log - GWLC - WBS - Popcorn - Coir [Re: cronicr]
    #21550702 - 04/15/15 11:31 AM (9 years, 1 month ago)


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