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blackdust


Registered: 02/28/09
Posts: 8,327
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Grow Log - GWLC - WBS - Popcorn - Coir
#21537459 - 04/12/15 01:50 PM (9 years, 1 month ago) |
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Hello, Thought I would share the start of a bulk grow and the chase of an isolate. Here is how it began:
- Multispore syringe to agar
- 3 transfers made of the fastest, cleanest, most rizo looking sections
- By only doing a few transfers I was able to have the best growing mycelium while still leaving hundreds of varieties to find a clone
- A total of 5 agar dishes were used in inoculating all masters
- agar was made with agar powder, potato flakes, and green food coloring
Here is a 3rd plate after 11 days

- 8 master jars were inoculated with the agar wedges
- Grain use was WBS
- Steep the grains for 4 hours and 2 hours drying (did not need to steep this long)
- pickling lime and gypsum were added to the steep and lightly mixed in with a spoon without disturbing the grains
- Pressure cooked for 120 min at 15 PSI
- Inoculated on 3/26
Here are the jars on 3/26

- Temps are 72 - 80F
- light is 24 hours (to lazy to turn on and off lights)
- Shaken at 5% colonization 3/30
Here are the jars, 2 days after shacking 4/2

Jars on 4/3
  Jars on 4/5

Jars on 4/7

- An GWLC was also inoculated on 3/26
- Used 30% WBS grain water (from the prepared WBS grains) to 70% water
- agar wedge was shaken into the LC
- The sediment on the bottom is carmalization of the carbohydrates in the grain water and aid to the nutrition of the LC
- Here is the LC, while shacking the wedge into the mix
Here is the GWLC on 3/26
  
Here is the GWLC on 4/10

What will happen next?
- GWLC will be expanded to more LC using SHIPS and syringe filters
- Popcorn Bags will be created for spawn
- Several monos, tubs, and trays will be made
- Oven pasteurized, coir subs
- a mushroom cluster will be found and cloned for further isolating and testing
- a good isolate will be stored in a slant
Hope you enjoy, Blackdust
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 3 months
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Re: Grow Log - GWLC - WBS - Popcorn - Coir [Re: blackdust]
#21537465 - 04/12/15 01:52 PM (9 years, 1 month ago) |
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--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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blackdust


Registered: 02/28/09
Posts: 8,327
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Re: Grow Log - GWLC - WBS - Popcorn - Coir [Re: cronicr]
#21550702 - 04/15/15 11:31 AM (9 years, 1 month ago) |
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