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OfflineCollective roots
Collective roots
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Registered: 03/22/15
Posts: 146
Loc: Maine
Last seen: 6 years, 11 months
storing and multiplying king oyster mycelium
    #21524165 - 04/09/15 01:29 PM (9 years, 1 month ago)

So im trying to get some different ideas on how to store and multiply my king oyster mycelium. If any one could describe there preferred methods of choice that would be great. So far i made up about 6 petri dishes and about 4 slants as back up. I have then been transferring agar to grain and grain to sw substrate to fruit out. I do 4 grain jars per dish. The problem is i do 2 jars a week and store halve the dish for next week so each dish last for 2 weeks and by the time i get through them all there getting old. i have only found loss of vigor in the last one or 2 so i will make less. my new plan will be doing agar to grain and than next week use some colonized grain that i will be putting into bags save some for g2g and than back to agar next week.


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Re: storing and multiplying king oyster mycelium [Re: Collective roots]
    #21524405 - 04/09/15 02:30 PM (9 years, 1 month ago)

1/4 dish to GM (tip (from Lipa I think), chop your dish bits really small)

GM to 10xG2 (by weight so usually jar to 6-8 bags)

Each G2 makes 10 or 20 G3 bags for bulk sub.

You can keep the G2's in the fridge for 6 months -1 year. When you expand you "re-set the clock"

Use your G3's ASAP but ideally within 2 months - at the tail end of that you will need to up your spawn ratio or expect longer colonisation.

Parafilm your dishes and they should last for years in the fridge. I always leave mine at incubation temps for 48hrs after I use them - that way I can see if anything nasty got on the dish during transfer.


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OfflineCollective roots
Collective roots
Male

Registered: 03/22/15
Posts: 146
Loc: Maine
Last seen: 6 years, 11 months
Re: storing and multiplying king oyster mycelium [Re: solarity]
    #21528865 - 04/10/15 02:11 PM (9 years, 1 month ago)

O wow that's a long time for g2 in hibernation I will have to start going to g2 more than that would be way easer. I guess  I was worried about loss of vigor since I started with lq culture from spore works but I think they would be good. Thanks for the input


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