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OfflineKingBoletus
Stranger

Registered: 03/31/15
Posts: 40
Last seen: 1 year, 8 months
1st timer bulk and mono.
    #21527244 - 04/10/15 02:43 AM (9 years, 1 month ago)

Hey everyone, so this "dude" has learned an immense amount from browsing these forums for the past few months and wanted me to tell you for him, he says bravo/thanks. What a great community and resource. This is in hopes to continue the evolution and fine tuning of technique and hopefully leads to valuable information for swim and others.

After a long wait- the dude came across spore 3 syringes (Tapalpa, Transkei, and Golden Teacher). Props to a guy named cpt Max so far... after being robbed $100 from a supposedly reliable guy "Ralph". Intentions are to "bulk spawn", maybe grain2grain for the faster colonizing strain, and at least 3 large mono tubs (20 gallon).

3/26
Anyways, using WBS mixed with "fine" vermiculite (about 10:1 ratio) using mostly 1/2 gallon jars with polyfill with 3/8" holes in lids (also a few smaller jars).

WBS was soaked for ~24 hours, rinsed thoroughly in a mesh sack, then mixed with verm and packed into jars 3/4 of the way full. Foil was sealed over the lids, and make-shift pc'd for about 2 hours (temp was sustained over 220 degrees- digital temp probe maxed out lol).


3/27
After a day of cooling, jars were taken into a thoroughly cleaned closet, foil was taken off jars and inoculated through the polyfill hole with clean syringes (using alcohol swab between jars). Each jar got only 1.5-2 ml of spore solution /: (syringe shaken before hand).
Stupidly, jars were not shaken after PC...woops. Jars are sitting in an open-topped tub in closet with exposure to small amount of ambient sunlight. No heat source.


3/31
All 6 Transkei jars show growth, small white patches visible where inoculate was squirted down the side of jar. No growth spotted on Tapalpa nor GT jars.


4/3
All 6 Transkei jars show more growth (slowly), first signs of growth visible in GT jars, no growth at all in Tapalpa jars. Condensation spotted on all jars but no standing water. All jars were shook vigorously.


4/8
All jars show more growth (very slow), but still no signs for Tapalpa. Shook all jars again except for Tap.

4/9
More growth observed, except Tap :frown: (bunk syringe maybe?). Inoculated grain seems to be well distributed, so faster colonization is expected at this point. Also maybe worth noting: temps fluctuate from 70's-low 80's at day to high50's-low 60's at night.
-More pics will be added tomorrow!
-Any comments/ suggestions welcome, thanks for looking!

Edited by KingBoletus (05/19/15 12:42 AM)

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OfflineAlbertheisenhouer
Tryptamine doctor
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Registered: 10/26/13
Posts: 734
Loc: Holland
Last seen: 22 days, 7 hours
Re: 1st timer bulk and mono. [Re: KingBoletus]
    #21527274 - 04/10/15 03:07 AM (9 years, 1 month ago)

Spores germinate not optimal on wbs or rye. A better technique is to germinate the spores on agar, then transfer the agar wedges to the WBS. Spore syringes are useful for PF cakes. These cakes are pretty foolproof. Most people start with this tek and then move on to bulk techniques.

PS, we do not SWIM on this forum


--------------------

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OfflineMatt87
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Registered: 01/03/15
Posts: 3,339
Loc: Tennessee
Last seen: 3 months, 19 days
Re: 1st timer bulk and mono. [Re: KingBoletus]
    #21527275 - 04/10/15 03:08 AM (9 years, 1 month ago)

There is no makeshift PC ing. Do yourself a favor and get a PC to work with wbs!


--------------------

Once you understand the way broadly, you see it in all things. -Musashi

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InvisibleTheEaglesGift
The Nagual


Registered: 04/10/11
Posts: 10,554
Loc: Ixtlan, Mexico
Trusted Cultivator
Re: 1st timer bulk and mono. [Re: KingBoletus]
    #21527284 - 04/10/15 03:13 AM (9 years, 1 month ago)

Spores cant take longer than 2 weeks to germinate sometimes. Don't write any of your jars off til you hit 3 weeks.

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OfflineKingBoletus
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Registered: 03/31/15
Posts: 40
Last seen: 1 year, 8 months
Re: 1st timer bulk and mono. [Re: TheEaglesGift]
    #21528451 - 04/10/15 11:57 AM (9 years, 1 month ago)

Sweet, thanks for the fast feedback!

Quote:

Albertheisenhouer said:
Spores germinate not optimal on wbs or rye. A better technique is to germinate the spores on agar, then transfer the agar wedges to the WBS. Spore syringes are useful for PF cakes. These cakes are pretty foolproof. Most people start with this tek and then move on to bulk techniques.

PS, we do not SWIM on this forum




Well some guy down the street might have gotten a little greedy/too confident with skipping cakes and going to bulk- just wants to dive right into the middle of it all!

Good advice, agar cultures will most definitely be used next time. Also hearing more issues with WBS, so most likely whole rye grain next time.

Quote:

Matt87 said:
There is no makeshift PC ing. Do yourself a favor and get a PC to work with wbs!




True, a little anxiety has been felt due to it not being a true PC and one will be used in the future. However temps were maintained for 2 hours with temp ratings of a 8-10 PSI PC(~235 degrees)- fingers crossed:confused:

Quote:

TheEaglesGift said:
Spores cant take longer than 2 weeks to germinate sometimes. Don't write any of your jars off til you hit 3 weeks.




Thanks, this is reassuring!

4/10
Mycelium growth has bounced back this morning, so jars will be left alone until %100 colonization (or close to it). Also small seedling heat mat was added to give temp rise.


Transkei jars:

The Tap jars have no visible growth still, however there is a slightly whitish wet sludgy effect going on with some of the WBS. Perhaps the dude down the street is paranoid, but it might be bacteria?? Could also be waterlogged wbs getting expanded/slimy? One jar in particular has this more: (sorry, camera doesn't seem to want to take very clear shots).


GT jars show growth, but it is very wispy/ fluffy- not dense mycelium.


--------------------
Don't always believe what you think.

Edited by KingBoletus (04/10/15 04:23 PM)

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InvisibleTheEaglesGift
The Nagual


Registered: 04/10/11
Posts: 10,554
Loc: Ixtlan, Mexico
Trusted Cultivator
Re: 1st timer bulk and mono. [Re: KingBoletus]
    #21529092 - 04/10/15 03:28 PM (9 years, 1 month ago)

Take some more pictures of the second to last pictured jar.

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OfflineKingBoletus
Stranger

Registered: 03/31/15
Posts: 40
Last seen: 1 year, 8 months
Re: 1st timer bulk and mono. [Re: TheEaglesGift]
    #21529260 - 04/10/15 04:14 PM (9 years, 1 month ago)

Sure, here are better ones. This jar is being quarantined for now.


The black mark on the glass is dry erase sharpie btw.


--------------------
Don't always believe what you think.

Edited by KingBoletus (04/10/15 04:16 PM)

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InvisibleTheEaglesGift
The Nagual


Registered: 04/10/11
Posts: 10,554
Loc: Ixtlan, Mexico
Trusted Cultivator
Re: 1st timer bulk and mono. [Re: KingBoletus]
    #21529334 - 04/10/15 04:34 PM (9 years, 1 month ago)

That jar is pretty damn wet. Do the rest look like that? I think it has bacteria.

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Invisibleazur
God of Fuck
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Registered: 04/21/12
Posts: 28,103
Loc: Daid
Re: 1st timer bulk and mono. [Re: TheEaglesGift]
    #21529790 - 04/10/15 06:36 PM (9 years, 1 month ago)

Quote:

TheEaglesGift said:
That jar is pretty damn wet. Do the rest look like that? I think it has bacteria.




--------------------


A cube is NOT a cube.

FALL IN LOVE WITH LC
FOTTSE!!!
ALL NOOBS READ THIS!!!


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OfflineKingBoletus
Stranger

Registered: 03/31/15
Posts: 40
Last seen: 1 year, 8 months
Re: 1st timer bulk and mono. [Re: azur]
    #21533911 - 04/11/15 03:54 PM (9 years, 1 month ago)

That could explain it, but all of the jars should be of the same water saturation as it was prepared in one big batch. Maybe that one strain (Tapalpa) in particular isn't as resilient to higher saturation?

Think maybe a fan should be placed in the closet to speed up drying process of jars? Opening them once in a while would def bring moisture down but not worth contam. right?


--------------------
Don't always believe what you think.

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InvisibleTheEaglesGift
The Nagual


Registered: 04/10/11
Posts: 10,554
Loc: Ixtlan, Mexico
Trusted Cultivator
Re: 1st timer bulk and mono. [Re: KingBoletus]
    #21533928 - 04/11/15 03:59 PM (9 years, 1 month ago)

It's not that any one strain(variety is a better word) isn't resilient to higher water content, it's that that syringe may have had some bacteria in it(or you put the bacteria into the jar during inoculation).

Varieties technically have some genetics differences, but not in the sense that one variety would have issues with bacteria.

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OfflineMindSpelunker
Stranger
Registered: 04/10/15
Posts: 10
Last seen: 8 years, 11 months
Re: 1st timer bulk and mono. [Re: TheEaglesGift]
    #21602168 - 04/27/15 12:40 AM (9 years, 23 days ago)

Someone i know may have talked to ralph and be doing something extremely similar, except with grain bags and a Cambodian strain. Definitely cant wait to see how yours turns out!

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InvisibleTheEaglesGift
The Nagual


Registered: 04/10/11
Posts: 10,554
Loc: Ixtlan, Mexico
Trusted Cultivator
Re: 1st timer bulk and mono. [Re: MindSpelunker]
    #21602442 - 04/27/15 03:26 AM (9 years, 23 days ago)

Ralph?

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OfflineKingBoletus
Stranger

Registered: 03/31/15
Posts: 40
Last seen: 1 year, 8 months
Re: 1st timer bulk and mono. [Re: TheEaglesGift]
    #21647097 - 05/07/15 12:19 AM (9 years, 13 days ago)

OK, well this has been an invaluable learning experience if anything. Here is the story.. PS "Ralph" is associated with a vender- not very happy with him straight up robbing some dude of $100!!

First of all, the wbs was indeed too wet- (the importance in following a tek VERY closely cannot be stressed enough)In this case, the wbs was not dried enough before going into the jars, or was soaked for long (almost 2 days). This accounts for slow mycelium growth, and the occurrence of fermenting smells/ bacteria smells (stinky feet-like).

Every single jar of the GT strain has trich (the dude thinks). At first there was a wispy cotton-like growth towards the top of the jars mostly, the first diagnosis was "cobweb mold"- but no greying coloration and the smell is minty/earthy/catnippy which seems to be associated with trich (before it shows any green). Eventually the other jars stalled, however one "healthy" one was kept for ID and monitoring the mold through it's stages/lifecycles. However it is not going to be kept until sporulation. Here are some pics of an extremely healthy colony for reference:


Now, everything else was tossed out besides the 6 jars of Transkei. These jars had some growth, but stalled almost completely. As a last ditch attempt to salvage the project, the guy I know emptied all of the contents of the wbs jars onto a flat sanitized trashbag(Alcohol)in a sprayed down(Lysol)closet. Then, the solid "cakes" of mycelium were tossed into a large pot of tap water. After floating for a few minutes, each and just about every chunk of healthy looking mycelium was rubbed of most of the bacteria-laden wbs, dunked again and put on a paper towl.

Meanwhile, substrate was cooling down in a covered pot (70% coir(5-6 cups), 30% verm(2-3 cups), about 1 cup coffee grounds, 2-3 tbs gypsum, and enough boiling water to almost saturate everything. After cooled, substrate was squeezed till field capacity and placed in monotub with trashbag liner (everything wiped down with alcohol).

Mycelium chunks were tossed in, and broken up into nickel/pea/ sized fragments and thoroughly mixed with substrate, more substrate was cased on top to hide any mycelium (1/4" layer). The spawn to substrate ratio was about 50:50.

Monotub holes loosely stuffed with polyfill, placed into trashbag ,closed bag, and left to sit in closet.

To the dude's surprise, this is what is seen 4 days later! No bacteria smell either!


Some hyphal knots observed, colonization seems pretty fast for spawn which was just sitting in soggy bacteria-laden wbs. I know this is bad practice and the dude doesn't want to promote it. But in the name of science...:mushroom2:


--------------------
Don't always believe what you think.

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OfflineKingBoletus
Stranger

Registered: 03/31/15
Posts: 40
Last seen: 1 year, 8 months
Re: 1st timer bulk and mono. [Re: KingBoletus]
    #21671748 - 05/12/15 07:48 PM (9 years, 7 days ago)

Fruiting conditions started today!

Questions, comments, concerns welcome! The dude can use as much info as he can be given. Specifically regarding fanning/ spraying? (techniques, timing, signs).

Next.. some mycelium from this tub will be transferred into agar and grain water lc's will come into play. Rye berries and a pressure cooker are also in the mix for a much more successful attempt- the dude hopes!:crazy2:


--------------------
Don't always believe what you think.

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InvisibleTheEaglesGift
The Nagual


Registered: 04/10/11
Posts: 10,554
Loc: Ixtlan, Mexico
Trusted Cultivator
Re: 1st timer bulk and mono. [Re: KingBoletus]
    #21672847 - 05/13/15 12:10 AM (9 years, 7 days ago)

Quote:

Next.. some mycelium from this tub will be transferred into agar




You're better off cloning a pin/small fruit from the first flush to agar.

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OfflineKingBoletus
Stranger

Registered: 03/31/15
Posts: 40
Last seen: 1 year, 8 months
Re: 1st timer bulk and mono. [Re: TheEaglesGift]
    #21673037 - 05/13/15 01:39 AM (9 years, 7 days ago)

Noted, thank you


--------------------
Don't always believe what you think.

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OfflineKingBoletus
Stranger

Registered: 03/31/15
Posts: 40
Last seen: 1 year, 8 months
Re: 1st timer bulk and mono. [Re: KingBoletus]
    #21696711 - 05/18/15 11:41 PM (9 years, 1 day ago)

Got some pinnnns:cool:


--------------------
Don't always believe what you think.

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OfflineMatt87
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Registered: 01/03/15
Posts: 3,339
Loc: Tennessee
Last seen: 3 months, 19 days
Re: 1st timer bulk and mono. [Re: KingBoletus]
    #21696840 - 05/19/15 12:32 AM (9 years, 1 day ago)

Congrats!


--------------------

Once you understand the way broadly, you see it in all things. -Musashi

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OfflineKingBoletus
Stranger

Registered: 03/31/15
Posts: 40
Last seen: 1 year, 8 months
Re: 1st timer bulk and mono. [Re: Matt87]
    #21696858 - 05/19/15 12:40 AM (9 years, 1 day ago)

Quote:

Matt87 said:
Congrats!



Thanks buddy! It's an exciting day for the dude.:mushroom2:


--------------------
Don't always believe what you think.

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