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Offlineeldrud
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Registered: 05/22/06
Posts: 23
Loc: Canada Flag
Last seen: 8 years, 11 months
saving a strain
    #21456952 - 03/25/15 06:42 PM (9 years, 1 month ago)

from 2 dozen brf cakes and 3 lc's i have one very slow only 75% colonized brf cake left that actually colonized from 3 year old frozen samples of hydrated spores.

my question is when i do go to birth cake should i -
take a clone from myc and what do i put it in ? (no agar here)

trigger fruiting and take a tissue clone , agian put it into lc or grain ?

or my last option and my back up plan if i do get fruit will b spore printing as many caps as i can.

just reading and find alot of outdated teks ppl now scoff at, like honey lc tek i was told is terrible for cloning ...

any info from ppl who actually have saved a strain would be great !
thanks

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OfflinePsilicon
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Registered: 08/26/12
Posts: 7,057
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Re: saving a strain [Re: eldrud]
    #21456958 - 03/25/15 06:44 PM (9 years, 1 month ago)

Do you have a PC?  The agar link in my sig may help if you do.


--------------------
Agar - what, why and how?  Everything a beginner needs to know.
Oat Prep Tek
Bored?  Please take one of my experiments off my hands.

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Offlineeldrud
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Registered: 05/22/06
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Re: saving a strain [Re: Psilicon]
    #21456984 - 03/25/15 06:46 PM (9 years, 1 month ago)

yes i do have and use a pc.
i cant find agar and i dont buy stuff online,,,, although i might have to,  to get real about cultivation.

it was a great read btw , i thought it was 50.00$ a bag for agar agar lol.

Edited by eldrud (03/25/15 06:53 PM)

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OfflinePsilicon
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Re: saving a strain [Re: eldrud]
    #21457028 - 03/25/15 06:59 PM (9 years, 1 month ago)

Thanks.  :smile:

Yeah, agar agar is super cheap.  The high-end stuff can be expensive, but we (and a growing number of researchers, actually) can make do just fine with the food grade stuff.


--------------------
Agar - what, why and how?  Everything a beginner needs to know.
Oat Prep Tek
Bored?  Please take one of my experiments off my hands.

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Offlineeldrud
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Re: saving a strain [Re: Psilicon]
    #21520359 - 04/08/15 05:26 PM (9 years, 1 month ago)

No luck on finding agar near my local, online yea but i dont shop online...

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Offlinemushpunx
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Re: saving a strain [Re: eldrud]
    #21520380 - 04/08/15 05:29 PM (9 years, 1 month ago)

Try an ethnic asian grocery, if you live in a mid sized city you should have one.. one where they dont speak much english. Bring a photo on your phone cause if they dont speak english too well they might not know what you are looking for, at least thats how it is in my neighborhood


--------------------

Amateur Mycologists United
AMU Q&A

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InvisiblebodhisattaMDiscordReddit
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Re: saving a strain [Re: mushpunx]
    #21520392 - 04/08/15 05:33 PM (9 years, 1 month ago)

you can PC a thin layer of grain in a jar or a short and wide sterilizable container. and then put your clone tissue in the middle of the grains, then it will grow out and you can with a sterile tool remove some colonized grains and use it like a grain petri if you really cannot find agar anywhere.

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InvisibleInocuole
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Re: saving a strain [Re: eldrud]
    #21520462 - 04/08/15 05:51 PM (9 years, 1 month ago)

Quote:

eldrud said:
No luck on finding agar near my local, online yea but i dont shop online...




Is there a particular reason why not?  Dunno how I'd ever source synthetic filter discs or some of the specific syringes and needles I've needed without.


--------------------
                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

Coir-ca-cola
Jokes are funny until they're about you

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Offlineeldrud
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Re: saving a strain [Re: Inocuole]
    #21656859 - 05/09/15 11:07 AM (9 years, 11 days ago)

Update .
I have six mini rounds of oatmeal agar, slowly colonizing .
And my one and only BRF cake is pinning !!!!

Added a pic to my collection but cant post it on forum

Edited by eldrud (05/09/15 11:09 AM)

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OfflinePsilicon
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Registered: 08/26/12
Posts: 7,057
Last seen: 3 years, 4 months
Re: saving a strain [Re: eldrud]
    #21670307 - 05/12/15 02:57 PM (9 years, 8 days ago)



--------------------
Agar - what, why and how?  Everything a beginner needs to know.
Oat Prep Tek
Bored?  Please take one of my experiments off my hands.

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Offlineqz7624
-Q-
Registered: 01/04/15
Posts: 86
Last seen: 6 years, 7 months
Re: saving a strain [Re: Psilicon]
    #21670497 - 05/12/15 03:34 PM (9 years, 8 days ago)


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Offlineeldrud
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Re: saving a strain [Re: qz7624]
    #21671893 - 05/12/15 08:17 PM (9 years, 7 days ago)

thank you for helping me post Van  , i will try this again .

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OfflinecronicrFacebook
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Registered: 08/07/11
Posts: 61,436
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Re: saving a strain [Re: qz7624]
    #21672010 - 05/12/15 08:41 PM (9 years, 7 days ago)

Quote:

qz7624 said:




QFT


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor

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Offlineeldrud
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Registered: 05/22/06
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Last seen: 8 years, 11 months
Re: saving a strain [Re: cronicr] * 1
    #21672094 - 05/12/15 09:01 PM (9 years, 7 days ago)

http://ok i dunno if these are amazons or not but i got what i like to see in the looks department 100% ,so im very happy about that.

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InvisibleInocuole
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Re: saving a strain [Re: cronicr]
    #21672919 - 05/13/15 12:40 AM (9 years, 7 days ago)

Quote:

cronicr said:
Quote:

qz7624 said:




QFT




:themoreyouknow:


--------------------
                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

Coir-ca-cola
Jokes are funny until they're about you

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Offlineeldrud
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Registered: 05/22/06
Posts: 23
Loc: Canada Flag
Last seen: 8 years, 11 months
Re: saving a strain [Re: Inocuole]
    #21674616 - 05/13/15 01:16 PM (9 years, 7 days ago)

QFt  ?

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