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OfflinePinPornProducer
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Clone tissue question?
    #21511446 - 04/06/15 05:59 PM (9 years, 1 month ago)

So I just got done taking 3 tissue samples from a fruit and put them to agar and every piece completely blued. Are they going to work out?

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InvisiblebodhisattaMDiscordReddit
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Re: Clone tissue question? [Re: PinPornProducer]
    #21511464 - 04/06/15 06:01 PM (9 years, 1 month ago)

Yep!

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Re: Clone tissue question? [Re: bodhisatta]
    #21511472 - 04/06/15 06:02 PM (9 years, 1 month ago)

Quote:

bodhisatta said:
Yep!



:whathesaid:

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OfflinePinPornProducer
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Re: Clone tissue question? [Re: bodhisatta]
    #21511479 - 04/06/15 06:03 PM (9 years, 1 month ago)

Awesome, thought I fked up. I was easy with em too

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Re: Clone tissue question? [Re: PinPornProducer]
    #21511833 - 04/06/15 07:14 PM (9 years, 1 month ago)

Yeah. After a couple of days it'll turn completely white and really fuzzy as it starts to spread out onto the agar. Transfer as soon as you see contams. So far I've done one transfer of my current clone to get away from some trich. So far so good.

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InvisibleTheEaglesGift
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Re: Clone tissue question? [Re: dnllln]
    #21511987 - 04/06/15 07:43 PM (9 years, 1 month ago)

They'll be fine. Give them a few day sand they'll fluff out.

Watching clone tissue recover and crawl onto the agar is one of my favorite parts of mycology!

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Re: Clone tissue question? [Re: TheEaglesGift]
    #21523825 - 04/09/15 11:57 AM (9 years, 1 month ago)

They're bouncing back :thumbup:

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InvisibleTheEaglesGift
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Re: Clone tissue question? [Re: PinPornProducer]
    #21524677 - 04/09/15 03:32 PM (9 years, 1 month ago)

I knew they would.

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Offlinednllln
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Re: Clone tissue question? [Re: TheEaglesGift]
    #21524869 - 04/09/15 04:13 PM (9 years, 1 month ago)

I was nervous too when the sample turned completely dark blue. Yours is doing the same thing mine did. It'll fluff up completely into a white ball and grow out. Make sure you transfer when you see contams the goal being not to let the contam have enough time to sporulate so you can get a clean plate.

What are you planning to do with your clone?

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InvisibleTheEaglesGift
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Re: Clone tissue question? [Re: dnllln]
    #21524881 - 04/09/15 04:16 PM (9 years, 1 month ago)

Quote:

Make sure you transfer when before you see contams the goal being not to let the contam have enough time to sporulate so you can get a clean plate.




You want to transfer as fast as possible.

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Re: Clone tissue question? [Re: TheEaglesGift]
    #21524897 - 04/09/15 04:19 PM (9 years, 1 month ago)

Not trying to hijack your thread or anything man so sorry if i do, but is there anyway i can do clones of tissues without agar, im in the process of wanting to get into it but trying to find the right sources. Im doing all grains too btw so could i put the clone in a plastic bag than transfer to grain after it fuzzs? Thanks guys -kushroom


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OfflinePinPornProducer
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Re: Clone tissue question? [Re: dnllln]
    #21524915 - 04/09/15 04:25 PM (9 years, 1 month ago)

Quote:

dnllln said:
I was nervous too when the sample turned completely dark blue. Yours is doing the same thing mine did. It'll fluff up completely into a white ball and grow out. Make sure you transfer when you see contams the goal being not to let the contam have enough time to sporulate so you can get a clean plate.

What are you planning to do with your clone?



Gonna grow a tote with just the cloned culture after 2-3 transfers and see what the results are, make a few master jars and possibly slant some. I'm also going to try and get down to an isolate, I bought some mini monos so I can test em before going balls out on em

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InvisibleTheEaglesGift
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Re: Clone tissue question? [Re: PinPornProducer]
    #21525044 - 04/09/15 04:50 PM (9 years, 1 month ago)

Sometimes clones perform better as an intact clone rather than the sum of their parts(isolates). The different strains within the clone seem to work better in concert, rather than individually, though this is not always the case one way or the other.

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Re: Clone tissue question? [Re: TheEaglesGift]
    #21536343 - 04/12/15 08:34 AM (9 years, 1 month ago)

Well 1 of the 3 samples I took went bad, had some bacteria but the the other 2 look very healthy. Making some transfers today

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InvisibleTheEaglesGift
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Re: Clone tissue question? [Re: PinPornProducer]
    #21538670 - 04/12/15 06:12 PM (9 years, 1 month ago)

What do you mean one went bad? Pictures? Just curious.

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OfflinePinPornProducer
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Re: Clone tissue question? [Re: TheEaglesGift]
    #21538745 - 04/12/15 06:28 PM (9 years, 1 month ago)

Had some bacteria that turned the agar white in 2 days and started liquifying it. Smelled pretty rotten also

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InvisibleTheEaglesGift
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Re: Clone tissue question? [Re: PinPornProducer]
    #21538801 - 04/12/15 06:37 PM (9 years, 1 month ago)

Yeah, when that happens I think it's time to cut your strings to that plate. :lol:

I'd hate to have that happen to a rare or important culture. Oof.

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Re: Clone tissue question? [Re: TheEaglesGift]
    #21538948 - 04/12/15 07:15 PM (9 years, 1 month ago)

For sure, I made 3 hoping 1 would make it and 2 did so I did better than expected lol

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Re: Clone tissue question? [Re: kushroom]
    #21538976 - 04/12/15 07:22 PM (9 years, 1 month ago)

Quote:

kushroom said:
Not trying to hijack your thread or anything man so sorry if i do, but is there anyway i can do clones of tissues without agar, im in the process of wanting to get into it but trying to find the right sources. Im doing all grains too btw so could i put the clone in a plastic bag than transfer to grain after it fuzzs? Thanks guys -kushroom



I used to do it in lc's. Grain works too. Chances of success are not as high....but....check out this thread
http://www.shroomery.org/forums/showflat.php/Number/20964499


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Re: Clone tissue question? [Re: azur]
    #21543975 - 04/13/15 08:29 PM (9 years, 1 month ago)

If the original clone transfers look healthy and no sign of contams should I still transfer or let the plates grow out and then just transfer to grains?

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