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Ared
Stranger
Registered: 04/06/15
Posts: 13
Last seen: 9 years, 16 days
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New grower that's looked but time to ask
#21510880 - 04/06/15 03:39 PM (9 years, 1 month ago) |
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Ok so here's my deets.... strain pes amazonian substrate. Rye seed.... I soaked the rye seed for 8 hours instead of 24 then boiled them for 20 mins strained until no longer steaming.. not wet just moist... boiled my 1 quart canning jars for about 30 mins bc they were dirty... put rye seed in jars with two coffee filters under the lid bc a vid i watched said thatd be fine...then pressure cooker at 15 psi for about 75 mins... let cool to room temp. I put 3 inoculation holess bc I saw everyone saying two or four.. so averaged it lol... but about half a cc in each whole.... now I didn't have any duct tape to make a glove box... and don't have some awesome fan setup... so I took every thing out of my closet except a tote and sprayed inside with bleach water.. then hung a clean sheet around closet and sprayed inside of that then the tote again to sterilize the area.. let sit about 3 mins knocked the jars up in the tote in the closet with the sheet... now the needle wasn't long enough to go down into the substrate.. didn't know if that would affect them.. but now I'm reading that my info is outdated... do I losen the jar lids a bit for airflow? I have them in that closet.. with a heating pad on top of the tote bc it's a bit lower than 81 degrees probably like 75-78 the tote has a pattern on the bottom of it with holes... I have it sitting on a shelf u would put in a lockeR.. so it's off the ground. I know amazonian is supposed to be crazy fast but it's been 3 days wmith no sign of mycelium growth... where do you suggest storing the jars if I'm wrong? As long with any other pointers
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Buck513

Registered: 04/17/14
Posts: 5,682
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Re: New grower that's looked but time to ask [Re: Ared]
#21510928 - 04/06/15 03:49 PM (9 years, 1 month ago) |
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So you knocked up grain jars with ms? Next time PC your jars for at least 90 mins, 120 if you got the time. Bleach water isn't necessary and frankly the smell of it makes me nauseous. Most people use 70% Iso. If you're knocking up grain jars with ms it doesn't matter if the needle touches the grain. Just make sure to shake them after inoc to spread inoc points.
-------------------- Fail to plan and you plan to fail. Enter the Ban Lottery
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Ared
Stranger
Registered: 04/06/15
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Last seen: 9 years, 16 days
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Re: New grower that's looked but time to ask [Re: Buck513]
#21510953 - 04/06/15 03:57 PM (9 years, 1 month ago) |
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I've shaken em once a day should they be out in the light or what's up with my outdated info.. and maybe that's why I don't see mycelium bc it could be started more in the middle?
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Buck513

Registered: 04/17/14
Posts: 5,682
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Re: New grower that's looked but time to ask [Re: Ared]
#21510965 - 04/06/15 04:00 PM (9 years, 1 month ago) |
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Please don't shake your jars everyday
Shake once after inoculation, and one more time at around 20-30% colonization. Anymore than that and you're simply weakening the mycelium.
Also, remove that heater pad or whatever. 81f is too warm. Mid 70s is fine for colonization.
Anything that is colonizing be it a monotub, grain jars or whatever should receive natural indirect sunlight
Edited by Buck513 (04/06/15 04:04 PM)
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spacechildo
proletarians rise up



Registered: 01/24/13
Posts: 19,243
Loc: Babylon
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Re: New grower that's looked but time to ask [Re: Buck513]
#21510978 - 04/06/15 04:03 PM (9 years, 1 month ago) |
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I wouldn't shake after a MS inoculation, you just spread the spores so they can't find each other and mate as easily.
also OP; coffee filters arent any good, use SFDs or tight polyfill, you dont need tape for a SAB just make 2 arm holes in a clear tub or something.
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Ared
Stranger
Registered: 04/06/15
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Last seen: 9 years, 16 days
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Re: New grower that's looked but time to ask [Re: spacechildo]
#21510996 - 04/06/15 04:06 PM (9 years, 1 month ago) |
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by indirect light you mean sitting in a room away from the window somewhere?
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Buck513

Registered: 04/17/14
Posts: 5,682
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Re: New grower that's looked but time to ask [Re: spacechildo]
#21510998 - 04/06/15 04:06 PM (9 years, 1 month ago) |
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Quote:
spacechildo said: I wouldn't shake after a MS inoculation, you just spread the spores so they can't find each other and mate as easily.
also OP; coffee filters arent any good, use SFDs or tight polyfill, you dont need tape for a SAB just make 2 arm holes in a clear tub or something.
Ive only had 1 grain grow I used ms with. I shook half, didn't shakwe the other half. I deffinetly noticed the difference
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skiddy83
Stranger

Registered: 01/31/11
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Last seen: 7 years, 11 months
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Re: New grower that's looked but time to ask [Re: Ared]
#21511152 - 04/06/15 04:40 PM (9 years, 1 month ago) |
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I'm not going to tell you how to do stuff just give you help because a lot of people that post on forums don't know half of what they talk about and just start talking without doing research...
1st never rely completely on videos they are a good start but do a little research (reading) like on here also. Soak grain for at least 24 hours there is no hurry unless you want to waist time and supplys and start over. There is a bacteria called endospores that will destroy the whole project. I do mine a day to a day in a half just to make sure the endospores cracked all their shells. Like i said do what you want just trying to help. 12 hours probably work for some people but this is what i have done for years and 99 percent of the time if i get a contaminate the last thing i have to worry about is bacteria if anything its some type of mold instead...
2nd Never losen the lids! One, they already have air hole for oxygen exchange and two you don't want stuff to get in there. I would do a little reading on your lids because that is the most important part keeping MICROSCOPIC spores out of there (keywork Micro-Scopic). I drill a hole in the top about half inch or smaller and load some pillow stuffing in a socket, use flat head screw driver to drive the stuffing into the hole to where it is rock solid and sticking out both sides of the lid. I had a friend i was teaching this to and he got frustrated and kept blasting the stuff completely through the other side lol just takes practice but like i said you don't have to do my method just helping. do a little research and find out what types of tops you feel comfortable with..
3rd 78 degrees is the perfect temp and thing lower then that they slow down and anything higher then that they slow down too.. too much heat will start to cook it (remember this also when drying) because the active chemcial you are shooting for is really sesitive to light and heat.. def anything over a 100 is bad
4th I would store them at room temp for your first time or if anything put them on the pad on the lowest setting!! mycelium will make its own heat when colonizing..
If you want to watch some good videos then look up "Lets grow mushrooms" they are by rodger rabbit (shoomery name) and the guy is a legacy and god when it comes to mushrooms... he covers so many things in his videos i have them all and i want to say there are probably about 10 or 15 all together probably
And just a head up on your closet if it has carpet that is a bad idea.. thats like standing on a spunge loaded with MILLIONS of MOLD spores and blasting them in the air with you and your box... bathrooms are best and can be wiped down easy plus are small
Edited by skiddy83 (04/06/15 04:46 PM)
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spacechildo
proletarians rise up



Registered: 01/24/13
Posts: 19,243
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Re: New grower that's looked but time to ask [Re: skiddy83]
#21511172 - 04/06/15 04:45 PM (9 years, 1 month ago) |
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well,
1) foo mans wbs prep does not include a 24 hrs soak and gives great results for wbs, oats you just simply boil.
2) spot on!
3) I'm not that sure 78F is the no 1 temp, anything from 70-75 gives great results and anything from 65 or so is more than good enough. Dry your shrooms with heat, way over 100!
4) lets grow mushrooms kicks ass, but heat pads suck balls!
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skiddy83
Stranger

Registered: 01/31/11
Posts: 70
Last seen: 7 years, 11 months
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Re: New grower that's looked but time to ask [Re: spacechildo]
#21511265 - 04/06/15 05:15 PM (9 years, 1 month ago) |
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I quit messing with wbs the stuff here at the grain places was so nasty and need to be cleaned over and over.. i finally went back to organic rye which i only have to rinse once but organic anything is a lot better.. as far as oats i'm thinking of switching to because its cheaper then rye and works just as good and easy to find... 70 to 75 is good but regardless room temp is usually in that range anyway... I know people that use food dehydrator to dry them and they work because psilocybe isn't affected as much by the temp but the psilobin is which is what makes you trip... psilicybe is also converted to psilobin once its in your system...
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spacechildo
proletarians rise up



Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 8 months
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Re: New grower that's looked but time to ask [Re: skiddy83]
#21511274 - 04/06/15 05:17 PM (9 years, 1 month ago) |
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not by heat, by exposure to open air while being wet, and its the other way around with cin/cybin. but please lets not start another stupid dehydrator/no dehydrator crap thread
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skiddy83
Stranger

Registered: 01/31/11
Posts: 70
Last seen: 7 years, 11 months
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Re: New grower that's looked but time to ask [Re: spacechildo]
#21512837 - 04/06/15 10:45 PM (9 years, 1 month ago) |
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Not going to argue....
http://en.wikipedia.org/wiki/Psilocin
Psilocin is relatively unstable in solution due to its phenolic hydroxy (-OH) group. In the presence of oxygen it readily forms bluish and dark black degradation products[citation needed]. Similar products are also formed under acidic conditions in the presence of oxygen and Fe3+ ions (Keller's reagent).
Psilocin is the pharmacologically active agent in the body after ingestion of psilocybin or some species of psychedelic mushrooms.
Psilocybin is rapidly dephosphorylated in the body to psilocin which acts as a 5-HT2A, 5-HT2C and 5-HT1A agonist or partial agonist. Psilocin exhibits functional selectivity in that it activates phospholipase A2 instead of activating phospholipase C as the endogenous ligand serotonin does.[7] Psilocin is structurally similar to serotonin (5-HT),[8] differing only by the hydroxyl group being on the 4-position rather than the 5 and the dimethyl groups on the nitrogen. Its effects are thought to come from its partial agonist activity at 5-HT2A serotonin receptors in the prefrontal cortex.
Psilocin has no significant effect on dopamine receptors (unlike LSD) and only affects the noradrenergic system at very high dosages.[9]
Psilocin's half-life ranges from 1 to 3 hours.
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,867
Loc: ★
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Re: New grower that's looked but time to ask [Re: spacechildo]
#21512858 - 04/06/15 10:49 PM (9 years, 1 month ago) |
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Quote:
spacechildo said: not by heat, by exposure to open air while being wet, and its the other way around with cin/cybin. but please lets not start another stupid dehydrator/no dehydrator crap thread 
God damn it, I was all like "Maybe this thread will be okay"
Of course it's another fucking heat degradation bullshit ping pong match.
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Mycologist217
Frank's Disciple



Registered: 02/13/13
Posts: 2,425
Loc: Man of the Moon
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Re: New grower that's looked but time to ask [Re: Buck513]
#21512905 - 04/06/15 11:04 PM (9 years, 1 month ago) |
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Quote:
Buck513 said: Please don't shake your jars everyday
Shake once after inoculation, and one more time at around 20-30% colonization. Anymore than that and you're simply weakening the mycelium.
Also, remove that heater pad or whatever. 81f is too warm. Mid 70s is fine for colonization.
Anything that is colonizing be it a monotub, grain jars or whatever should receive natural indirect sunlight
-------------------- My LC Manual (With custom LC lid Tek) ~~ Required Mycology Supplies ~~ Agar Work Videos ~~ L G M AMU Q&A-NO SYMPATHY FOR THE DEVIL! KEEP THAT IN MIND! BUY THE TICKET: TAKE THE RIDE Check out my Retail Gourmet Mushroom Farm! Mycologist217 is a fictitious entity that uses images supplied by Google to mask his/her inability to develop normal social habits.
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silverstem
Caps & Stems


Registered: 10/12/13
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Re: New grower that's looked but time to ask [Re: skiddy83]
#21512923 - 04/06/15 11:10 PM (9 years, 1 month ago) |
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Quote:
skiddy83 said: I'm not going to tell you how to do stuff just give you help because a lot of people that post on forums don't know half of what they talk about and just start talking without doing research...
1st never rely completely on videos they are a good start but do a little research (reading) like on here also. Soak grain for at least 24 hours there is no hurry unless you want to waist time and supplys and start over. There is a bacteria called endospores that will destroy the whole project. I do mine a day to a day in a half just to make sure the endospores cracked all their shells. Like i said do what you want just trying to help. 12 hours probably work for some people but this is what i have done for years and 99 percent of the time if i get a contaminate the last thing i have to worry about is bacteria if anything its some type of mold instead...
That's not true.. I never soak my grains....NEVER!.. I simply simmer for 15 min drain then fill the pan back up then simmer for 15 min then drain for 15 min then fill my jars... By that time they have puffed up enough. If u simmer for too long ur grains will crack that's why I only do it for 15... Not every tek calls for soak. They come out perfect every time.... I also only PC for 1 hour... Never had a contamed jar..
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,867
Loc: ★
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Re: New grower that's looked but time to ask [Re: skiddy83]
#21512934 - 04/06/15 11:14 PM (9 years, 1 month ago) |
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Quote:
skiddy83 said: I'm not going to tell you how to do stuff just give you help because a lot of people that post on forums don't know half of what they talk about and just start talking without doing research...
Quote:
skiddy83 said: I quit messing with wbs the stuff here at the grain places was so nasty and need to be cleaned over and over.. i finally went back to organic rye which i only have to rinse once but organic anything is a lot better.. as far as oats i'm thinking of switching to because its cheaper then rye and works just as good and easy to find... 70 to 75 is good but regardless room temp is usually in that range anyway... I know people that use food dehydrator to dry them and they work because psilocybe isn't affected as much by the temp but the psilobin is which is what makes you trip... psilicybe is also converted to psilobin once its in your system...
Irronyyyyyy
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silverstem
Caps & Stems


Registered: 10/12/13
Posts: 900
Loc: jordan
Last seen: 6 years, 9 months
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Re: New grower that's looked but time to ask [Re: Inocuole]
#21512938 - 04/06/15 11:15 PM (9 years, 1 month ago) |
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Quote:
Inocuole said:
Quote:
skiddy83 said: I'm not going to tell you how to do stuff just give you help because a lot of people that post on forums don't know half of what they talk about and just start talking without doing research...
Quote:
skiddy83 said: I quit messing with wbs the stuff here at the grain places was so nasty and need to be cleaned over and over.. i finally went back to organic rye which i only have to rinse once but organic anything is a lot better.. as far as oats i'm thinking of switching to because its cheaper then rye and works just as good and easy to find... 70 to 75 is good but regardless room temp is usually in that range anyway... I know people that use food dehydrator to dry them and they work because psilocybe isn't affected as much by the temp but the psilobin is which is what makes you trip... psilicybe is also converted to psilobin once its in your system...
Irronyyyyyy
 
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Shroomery needs a gun forum!!!!!!!!! CAN WE HAVE ONE?????
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cronicr



Registered: 08/07/11
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Loc: Van Isle
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Re: New grower that's looked but time to ask [Re: silverstem]
#21512970 - 04/06/15 11:28 PM (9 years, 1 month ago) |
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oh man another one of these threads
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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skiddy83
Stranger

Registered: 01/31/11
Posts: 70
Last seen: 7 years, 11 months
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Re: New grower that's looked but time to ask [Re: Inocuole]
#21513036 - 04/06/15 11:47 PM (9 years, 1 month ago) |
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Quote:
Inocuole said:
Quote:
skiddy83 said: I'm not going to tell you how to do stuff just give you help because a lot of people that post on forums don't know half of what they talk about and just start talking without doing research...
Quote:
skiddy83 said: I quit messing with wbs the stuff here at the grain places was so nasty and need to be cleaned over and over.. i finally went back to organic rye which i only have to rinse once but organic anything is a lot better.. as far as oats i'm thinking of switching to because its cheaper then rye and works just as good and easy to find... 70 to 75 is good but regardless room temp is usually in that range anyway... I know people that use food dehydrator to dry them and they work because psilocybe isn't affected as much by the temp but the psilobin is which is what makes you trip... psilicybe is also converted to psilobin once its in your system...
Irronyyyyyy
Yep 
http://en.wikipedia.org/wiki/Psilocin
Quote:
Inocuole said:
Quote:
skiddy83 said: I'm not going to tell you how to do stuff just give you help because a lot of people that post on forums don't know half of what they talk about and just start talking without doing research...
Quote:
skiddy83 said: I quit messing with wbs the stuff here at the grain places was so nasty and need to be cleaned over and over.. i finally went back to organic rye which i only have to rinse once but organic anything is a lot better.. as far as oats i'm thinking of switching to because its cheaper then rye and works just as good and easy to find... 70 to 75 is good but regardless room temp is usually in that range anyway... I know people that use food dehydrator to dry them and they work because psilocybe isn't affected as much by the temp but the psilobin is which is what makes you trip... psilicybe is also converted to psilobin once its in your system...
Irronyyyyyy
Yep dang i hate being right 
See if your going to say something back it up with knowledge and proof http://en.wikipedia.org/wiki/Psilocin
and as far as not ever soaking thats a new one on me silverstem and thanks I just looked it all up and the dif is if someone doesn't have a PC they can soak the grain and boil it to kill the endospores but a PC that goes up to 15 psi will kill them so you don't have to soak them technically but me personally i will still soak so that i know its all dead plus it hydrates them really well
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,867
Loc: ★
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Re: New grower that's looked but time to ask [Re: skiddy83]
#21513062 - 04/06/15 11:52 PM (9 years, 1 month ago) |
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I was making fun of the fact that you couldn't spell either chemical name correctly without using a wiki. But, yeah, let's pretend I don't know that stuff for fun.
If anything that makes it funnier.
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