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OfflineHaywire
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ancient mycelium fruiting attempt
    #21509599 - 04/06/15 12:25 PM (5 years, 10 months ago)

So I got back from a couple months traveling abroad and I found some leftover mycelium in my parents shed where I stored all my mycology stuff.



As I'm waiting for my AA pc to arrive I thought might as well try fruiting this ancient mycelium and see what happens.

I pasteurized in jars the way horrigan does it.



the myc did smell really good though



So here goes. I know it's contaminated so don't bother. I'm just having a bit of fun. let's see what happens.



Update 8/4:

I had a little peek and the mycelium seems to be recovering. hopefully it stays contam free!!



Update 14/4:

I had another peek. Not completely colonized yet but looks ok to me. I even have two premature pins.



Update 17/4:

It's fruiting. sub doesn't seem to be completely colonized yet but I've got some pins. I'm gonna introduce it to fruiting conditions. I'm not casing. should I remove the pins?



Update 22/4

So first flush came through. around 140 grams wet. Not too shabby for ancient forgotten mycelium.



I found the jars with visible contaminations, a clear liquid, probably mycelial metabolites, and (dead) in vitro mushrooms. After hydrating the myc it produced a first flush. I consider this experiment to be closed. If more flushes appear I'll update further.

thanks for following


Edited by Haywire (04/22/15 07:03 AM)


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Re: ancient mycelium fruiting attempt [Re: Haywire]
    #21509601 - 04/06/15 12:26 PM (5 years, 10 months ago)

:popcorn:


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Re: ancient mycelium fruiting attempt [Re: wowimflabbergasted]
    #21509611 - 04/06/15 12:28 PM (5 years, 10 months ago)

:popcorn: bet it fruits eventually


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Re: ancient mycelium fruiting attempt [Re: wowimflabbergasted]
    #21509612 - 04/06/15 12:28 PM (5 years, 10 months ago)

looks dry as fuck...


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Re: ancient mycelium fruiting attempt [Re: maui]
    #21509630 - 04/06/15 12:32 PM (5 years, 10 months ago)

should have saved one of those for g2g. now you will have to start from step 1.
I have used colonized grain a few month old for g2g.You just need to sterilize a spoon to get it out.


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Re: ancient mycelium fruiting attempt [Re: bw86]
    #21509731 - 04/06/15 01:00 PM (5 years, 10 months ago)

dunk n roll


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Re: ancient mycelium fruiting attempt [Re: maui]
    #21509893 - 04/06/15 01:32 PM (5 years, 10 months ago)

Quote:

bw86 said:
should have saved one of those for g2g. now you will have to start from step 1.
I have used colonized grain a few month old for g2g.You just need to sterilize a spoon to get it out.




Like I said, no pc yet so I can't do any agar work. Used a knife, didn't sterilize it :smile:

Quote:

rxb said:
dunk n roll




Did dunk mixed it with very and coir.

Quote:

maui said:
looks dry as fuck...




wasn't dry at all.


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Re: ancient mycelium fruiting attempt [Re: Haywire]
    #21509907 - 04/06/15 01:35 PM (5 years, 10 months ago)

you don't need a PC for agar


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Re: ancient mycelium fruiting attempt [Re: bodhisatta]
    #21509925 - 04/06/15 01:38 PM (5 years, 10 months ago)

Quote:

bodhisatta said:
you don't need a PC for agar



Go on..


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Re: ancient mycelium fruiting attempt [Re: wowimflabbergasted]
    #21509944 - 04/06/15 01:42 PM (5 years, 10 months ago)

sterilize it without a PC pour into dishes, use !


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Re: ancient mycelium fruiting attempt [Re: bodhisatta]
    #21509952 - 04/06/15 01:43 PM (5 years, 10 months ago)

Just steam sterilize?


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Re: ancient mycelium fruiting attempt [Re: wowimflabbergasted]
    #21509955 - 04/06/15 01:44 PM (5 years, 10 months ago)

yes


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Re: ancient mycelium fruiting attempt [Re: bodhisatta]
    #21509963 - 04/06/15 01:45 PM (5 years, 10 months ago)

For what, 2 hours?


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Re: ancient mycelium fruiting attempt [Re: bodhisatta]
    #21509965 - 04/06/15 01:45 PM (5 years, 10 months ago)

:popcorn:


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Re: ancient mycelium fruiting attempt [Re: grinsmiley]
    #21509971 - 04/06/15 01:47 PM (5 years, 10 months ago)

30-45m. 10-15 in a PC.


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Re: ancient mycelium fruiting attempt [Re: bodhisatta]
    #21509983 - 04/06/15 01:48 PM (5 years, 10 months ago)

:thumbup:


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Re: ancient mycelium fruiting attempt [Re: wowimflabbergasted]
    #21509996 - 04/06/15 01:51 PM (5 years, 10 months ago)

agar is easy to sterilize.

some lab grade agar actually can't be autoclaved so the directions tell you to microwave it, I wouldn't do that on the regular but

most lab grade agar right on the bottle tells you 10 or 15m.
some are even as low as 5m

https://www.sargentwelch.com/www.sargentwelch.com/images/Pouring_Plates.pdf

I usually do 10-15m at 15psi

agar agar and the nutrients you put in contain no heat resistant bacteria or endospores you have to worry about.


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Re: ancient mycelium fruiting attempt [Re: bodhisatta]
    #21510023 - 04/06/15 01:58 PM (5 years, 10 months ago)

too late anyway!


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Re: ancient mycelium fruiting attempt [Re: Haywire]
    #21510030 - 04/06/15 01:58 PM (5 years, 10 months ago)

It's never too late. :bigblunt:


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Re: ancient mycelium fruiting attempt [Re: wowimflabbergasted]
    #21510068 - 04/06/15 02:09 PM (5 years, 10 months ago)

haha it's already moved to bulk.
don't want to see the rainbow that'll give on agar :smile:


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