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InvisibleAquaticPuzzle
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Can I?
    #21503983 - 04/04/15 08:33 PM (9 years, 1 month ago)

Can I do anything with this print like pf tek or mix it with some water and spray it in my yard?





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Offlinemushpunx
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Re: Can I? [Re: AquaticPuzzle]
    #21503993 - 04/04/15 08:37 PM (9 years, 1 month ago)

You could put it to agar and try to clean up a culture, since you didnt take it under sterile condition I wouldnt use it for a syringe


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InvisibleAquaticPuzzle
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Re: Can I? [Re: mushpunx]
    #21503997 - 04/04/15 08:43 PM (9 years, 1 month ago)

Thanks I will look into agar.Are you saying I should use tinfoil in stead of a piece of paper?This was taken with cleanliness in mind also in a makeshift SAB.


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OfflinePinPornProducer
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Re: Can I? [Re: AquaticPuzzle]
    #21504038 - 04/04/15 08:59 PM (9 years, 1 month ago)

I pc pre cut pieces of foil and keep them in the jar until I'm ready to take prints which I do inside my glove box to try and stay as clean as possible. You will never be 100% sterile taking prints but the cleaner you can be the easier it is to get going wether it be on agar or syringe

Edited by PinPornProducer (04/04/15 09:02 PM)

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Offlinemushpunx
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Re: Can I? [Re: AquaticPuzzle]
    #21504058 - 04/04/15 09:05 PM (9 years, 1 month ago)

Quote:

AquaticPuzzle said:
Thanks I will look into agar.Are you saying I should use tinfoil in stead of a piece of paper?This was taken with cleanliness in mind also in a makeshift SAB.





Oh well it might be clean actually I just assumed it was out of the SAB with the air settling down on it and whatnot did you cover it?

But yea, foil is sterile enough for prints if you tear off a sheet first and toss it

And ^ what he said .. just makes it easier to clean on agar. If you have a real clean print you cam make a syringe (which you still should put to agar but if youre doing say PF tek, brf is usually forgiving enough for syringe)


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InvisibleAquaticPuzzle
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Re: Can I? [Re: PinPornProducer]
    #21504073 - 04/04/15 09:10 PM (9 years, 1 month ago)

Ok so paper is a no but it was taken from the middle of the book am I going to have to isolate or can i just use any clean culture and can i go from agar to pf tek or do you have to do grains?Thanks for helping me I am new to mycology.
Also I will be reading Stro's Cleaning and Isolating On Agar do I need to make a glovebox for agar or will a SAB do?


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InvisibleAquaticPuzzle
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Re: Can I? [Re: mushpunx]
    #21504088 - 04/04/15 09:14 PM (9 years, 1 month ago)

Inside a SAB underneath a clean shotglass folded up and bagged in the SAB.


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Offlinemushpunx
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Re: Can I? [Re: AquaticPuzzle]
    #21504312 - 04/04/15 10:25 PM (9 years, 1 month ago)

You dont have to isolate but you wanna at least make a few transfers before putting your culture to grain.

Its kinda hard to put agar to PF tek.

I doubt the surface of the paper was sterile which might make your spore syringe dirty, which is why I recomend agar because it is a great tool that allows you to clean a culture.


Like I think an outside print you always wanna put to agar, indoor takem under good conditions you could chance to putting to syringe


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Edited by mushpunx (04/04/15 10:32 PM)

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InvisibleGrey
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Re: Can I? [Re: mushpunx]
    #21504331 - 04/04/15 10:31 PM (9 years, 1 month ago)

You could make an easy LI with a colonized agar dish, and shoot that into some pf cakes. But if you have a PC to sterilize agar, just make up some grain jars and go big.


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Invisible2shoes
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Re: Can I? [Re: mushpunx]
    #21504339 - 04/04/15 10:34 PM (9 years, 1 month ago)

I hear you can PC parchment paper and the spores can be "poured" into a shot glass then syringes up with sterile water. Haven't done it but seems plausible for PF's..:shrug:

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Offlinemushpunx
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Re: Can I? [Re: 2shoes]
    #21504431 - 04/04/15 11:05 PM (9 years, 1 month ago)

Quote:

newbie2shoes said:
I hear you can PC parchment paper and the spores can be "poured" into a shot glass then syringes up with sterile water. Haven't done it but seems plausible for PF's..:shrug:





I dont know what you mean about this but if you are talking about a spore syringe all you need to do is scrape off some spores into sterile water to suck up, this is standard practice for making a spore syringe from print. 

One print can make 10-20 syringes. But they are worthless if they aremt clean


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Invisible2shoes
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Re: Can I? [Re: mushpunx]
    #21504446 - 04/04/15 11:10 PM (9 years, 1 month ago)

Quote:

mushpunx said:
Quote:

newbie2shoes said:
I hear you can PC parchment paper and the spores can be "poured" into a shot glass then syringes up with sterile water. Haven't done it but seems plausible for PF's..:shrug:





I dont know what you mean about this but if you are talking about a spore syringe all you need to do is scrape off some spores into sterile water to suck up, this is standard practice for making a spore syringe from print. 

One print can make 10-20 syringes. But they are worthless if they aremt clean




Essentially skipping the scraping step since the fall off of the paper into the water..

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Offlinemushpunx
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Re: Can I? [Re: 2shoes]
    #21504456 - 04/04/15 11:15 PM (9 years, 1 month ago)

Like squirting some sterile water onto print, swirling a loop around to loosen the spores and then pour into the water?

Yea that would work thats how I make my syringes


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InvisibleAquaticPuzzle
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Re: Can I? [Re: 2shoes]
    #21504475 - 04/04/15 11:21 PM (9 years, 1 month ago)

Can this species be grown on pf cakes?


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OfflinePinPornProducer
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Re: Can I? [Re: mushpunx]
    #21504483 - 04/04/15 11:23 PM (9 years, 1 month ago)

Print inside sterilized 1/2 pint jars and you can just put sterile water into the jar, stir it up and fill your syringes

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InvisibleAquaticPuzzle
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Re: Can I? [Re: PinPornProducer]
    #21504498 - 04/04/15 11:27 PM (9 years, 1 month ago)

Sorry I do not have a PC so I cant do that that's why I am going for pf cakes.


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Re: Can I? [Re: AquaticPuzzle]
    #21504965 - 04/05/15 03:54 AM (9 years, 1 month ago)

Any particular reason you want to grow that shroom out? I don't think it is active. Im not the best ID person out there but it looks like panaeolus papilionaceus.

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Invisible2shoes
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Re: Can I? [Re: Sockadin]
    #21505240 - 04/05/15 07:57 AM (9 years, 1 month ago)

Fish hooks and some line for the use of half pint to lower the cap into the jar, but the shorty 4oz may be easier.. just PC the foil in the jar that your using.

Have an injection port lid :rockon:

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Offlinemushpunx
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Re: Can I? [Re: AquaticPuzzle]
    #21505735 - 04/05/15 10:41 AM (9 years, 1 month ago)

Quote:

AquaticPuzzle said:
Can this species be grown on pf cakes?






I just assumed you knew what species it was. Ps. Cubensis, pan cyan/pan cambo/ps mexicana and all the different varieties grow well indoors.


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InvisibleAquaticPuzzle
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Re: Can I? [Re: Sockadin]
    #21507327 - 04/05/15 06:34 PM (9 years, 1 month ago)

I think this one is Panaeolus olivaceus that is what a TI told me but I was hoping for more input on the species.It was found growing in a grassy area next to the river PNW.I did not see any bruising how can I tell the difference?


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