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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,866
Loc: Canada
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Re: The Visual Signs Of A Contaminated Liquid Culture. [Re: camplo] 1
#21502908 - 04/04/15 03:25 PM (9 years, 1 month ago) |
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TL:DR
Yeah I know camplo, your way is best no question. Anyone doing otherwise is an idiot. Check
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invitro

Registered: 05/03/13
Posts: 2,529
Last seen: 5 months, 6 days
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Re: The Visual Signs Of A Contaminated Liquid Culture. [Re: bodhisatta]
#21502942 - 04/04/15 03:34 PM (9 years, 1 month ago) |
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Quote:
bodhisatta said: Some infections will not be visable or smellable and will not change the turbidity and be undetectable
Which infections are you referring to? what species?
Edited by invitro (04/04/15 03:35 PM)
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,891
Loc: Milky way
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Re: The Visual Signs Of A Contaminated Liquid Culture. [Re: invitro]
#21502966 - 04/04/15 03:39 PM (9 years, 1 month ago) |
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Anything depends on how it grows. Pedio and homofermentative lacto would be undetectable likely in normal sugar % that LC uses. Bacteria behaves very differently depending what's available which stressors it has to deal with, temp, and disolvex oxygen levels.
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Re: The Visual Signs Of A Contaminated Liquid Culture. [Re: camplo]
#21502990 - 04/04/15 03:44 PM (9 years, 1 month ago) |
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this is the way i think as well. i try to use whatever is easiest and most efficient.
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invitro

Registered: 05/03/13
Posts: 2,529
Last seen: 5 months, 6 days
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Re: The Visual Signs Of A Contaminated Liquid Culture. [Re: bodhisatta]
#21502998 - 04/04/15 03:45 PM (9 years, 1 month ago) |
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How could any bacteria hide in a lc, seeing how they multiply very quickly. It seems like that mass of billions of bacteria would have to have some kind of visual signature.
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Re: The Visual Signs Of A Contaminated Liquid Culture. [Re: bodhisatta]
#21503006 - 04/04/15 03:47 PM (9 years, 1 month ago) |
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do you have any pictures of any of the contaminants? that would be a good thing to post in this thread. kizzle has a pretty good collection of contaminant pics hes compiling in his journal. that would be a cool thing to show in lc's.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,891
Loc: Milky way
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Re: The Visual Signs Of A Contaminated Liquid Culture. [Re: invitro]
#21503025 - 04/04/15 03:51 PM (9 years, 1 month ago) |
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Quote:
invitro said: How could any bacteria hide in a lc, seeing how they multiply very quickly. It seems like that mass of billions of bacteria would have to have some kind of visual signature.
Depends some things like ecoli reproduce like that lactococcus sp. Some have day long splitting times. Depends on avail nutrition too.
I'll post some pictures of lc seeded with different things I have some pictures of bacteria from LC made with MRS broth and YPD broth I'll post pics of the see thru clear lc too next time
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,866
Loc: Canada
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Re: The Visual Signs Of A Contaminated Liquid Culture. [Re: invitro]
#21503033 - 04/04/15 03:53 PM (9 years, 1 month ago) |
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bodhi has some great microscopy pics of bacteria in LC. He has posted them a few times.
I once had an LC that developed a yeast contam which I traced to a cracked jar rim, that looked fine. Looked healthy. I shot up a bunch of jars and they colonized great. Went back to the LC a week later which still looked awesome and had no recovery with the second round. Decided to dump it out and at that point realized that it smelled like liquor. But it looked great.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,891
Loc: Milky way
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Re: The Visual Signs Of A Contaminated Liquid Culture. [Re: bodhisatta]
#21503038 - 04/04/15 03:54 PM (9 years, 1 month ago) |
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like this isn't millions and millions this is example of about 10,000 per ml bacteria if it was cloudy from bacteria it would be more like 10^7 or more
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Re: The Visual Signs Of A Contaminated Liquid Culture. [Re: Pastywhyte] 1
#21503043 - 04/04/15 03:55 PM (9 years, 1 month ago) |
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might also be worth letting the bacteria grow throughout the lc so you can see what it looks like on the large scale as well. to the human eye that is. and you know throw it out after for the sake of the picture.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,891
Loc: Milky way
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Re: The Visual Signs Of A Contaminated Liquid Culture. [Re: eatyualive]
#21503122 - 04/04/15 04:15 PM (9 years, 1 month ago) |
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That's all it grew up to
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,867
Loc: ★
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Re: The Visual Signs Of A Contaminated Liquid Culture. [Re: bodhisatta]
#21503529 - 04/04/15 06:03 PM (9 years, 1 month ago) |
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Having that much bacteria is way beyond the point of failure and is not visibly detectable either. Letting it get worse until you can tell with your eyes doesn't seem to be the most efficient method either.
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hamloaf
Pork Block ®©™√


Registered: 12/23/09
Posts: 21,754
Loc: ation undisclosed.
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Re: The Visual Signs Of A Contaminated Liquid Culture. [Re: bodhisatta] 1
#21503537 - 04/04/15 06:04 PM (9 years, 1 month ago) |
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Quote:
bodhisatta said: Some infections will not be visable or smellable and will not change the turbidity and be undetectable
I meant if there is any contam present on the encapsulated grains, and, or a thwart in sterile procedure of the inoculation of said LC that the LC will breed the infection like rabbits, and become infected. No where to run.
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DirdyD


Registered: 12/01/14
Posts: 1,650
Last seen: 3 years, 3 months
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Re: The Visual Signs Of A Contaminated Liquid Culture. [Re: hamloaf]
#21503561 - 04/04/15 06:11 PM (9 years, 1 month ago) |
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I love Bodhi's microscopy photos.
Looking to get my lab set up proper in the next few months...
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,891
Loc: Milky way
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Re: The Visual Signs Of A Contaminated Liquid Culture. [Re: DirdyD]
#21503581 - 04/04/15 06:16 PM (9 years, 1 month ago) |
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spring for a phase contrast microscope if you can pony the money.
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hamloaf
Pork Block ®©™√


Registered: 12/23/09
Posts: 21,754
Loc: ation undisclosed.
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Re: The Visual Signs Of A Contaminated Liquid Culture. [Re: eatyualive] 1
#21503612 - 04/04/15 06:24 PM (9 years, 1 month ago) |
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Quote:
eatyualive said: when you use this lc gun do you intend to give the lc a good stir on the plate for a few hours prior to using it in case of any clogging.
man, this is going to be fucking epic!!

Yes, the LC is intended to be stirred on the stirplate for a while just before aspiration. I'm pretty stoked to begin research with this gun. Next obsticle is going to safely lubricating the trigger with the right lubricant.
In comparison to needle/syringe work? The clogging of this LC gun seems virtually impossible. The tube to the LC gun is 1/4" thick. Don't know how wide the syringe barrel to the LC gun is, exactly. Can tell you that the syringe barrel is wider than 10-12cc syringes. Not as wide as 60cc syringes though. The unit has an interchanging luer locking system, so any gauge needle can be employed to dispense the culture with.
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DirdyD


Registered: 12/01/14
Posts: 1,650
Last seen: 3 years, 3 months
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Re: The Visual Signs Of A Contaminated Liquid Culture. [Re: bodhisatta]
#21503651 - 04/04/15 06:39 PM (9 years, 1 month ago) |
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Quote:
bodhisatta said: spring for a phase contrast microscope if you can pony the money.
Hell yeah big boy toys. Take one of these over a jet ski any day.
Brand suggestion?
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,891
Loc: Milky way
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Re: The Visual Signs Of A Contaminated Liquid Culture. [Re: DirdyD]
#21503659 - 04/04/15 06:43 PM (9 years, 1 month ago) |
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nikon
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,891
Loc: Milky way
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Re: The Visual Signs Of A Contaminated Liquid Culture. [Re: bodhisatta]
#21503666 - 04/04/15 06:45 PM (9 years, 1 month ago) |
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http://www.singerinstruments.com/solution/sporeplay/
if you could afford this it would be extremely useful. you could do single spore studies and make varietal hybrids with ease. but with the extra long focal distance objectives you can view petri dishes at 400x which is crazy.
yeast cells are about the size of cube spores. the spores from the yeast are way smaller, just for reference.
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DirdyD


Registered: 12/01/14
Posts: 1,650
Last seen: 3 years, 3 months
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Re: The Visual Signs Of A Contaminated Liquid Culture. [Re: bodhisatta]
#21503685 - 04/04/15 06:54 PM (9 years, 1 month ago) |
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Thanks for the heads up.
Edited by DirdyD (04/04/15 07:23 PM)
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