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SporeWhore69
Stranger


Registered: 02/21/15
Posts: 7
Last seen: 9 years, 29 days
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need help identifying whether or not this is contaminated
#21492450 - 04/02/15 01:48 PM (9 years, 1 month ago) |
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Hello fine folks of shroomery, currently two PF jars are looking a little off compared to the rest of them. I need to know if this is contaminated or will be just fine thank you!

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Mr. Magic



Registered: 07/13/14
Posts: 1,951
Last seen: 2 days, 15 hours
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Re: need help identifying whether or not this is contaminated [Re: SporeWhore69]
#21492522 - 04/02/15 02:08 PM (9 years, 1 month ago) |
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How long since they've been inoculated?? I feel like i see a green dot in the middle of the top pic but i would just tell u to keep watching them and maybe isolate them from your other jars. The yellow looks to me like its just piss from the mycelium but it does look funky. Just move them from your other jars and check in another 3-4 days, maybe take pics then and post.
What was your procedure for making the substrate, sterilizing, etc. How did you shoot them up with the spores? Did you make sure you were in a sterile environment?
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SporeWhore69
Stranger


Registered: 02/21/15
Posts: 7
Last seen: 9 years, 29 days
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Re: need help identifying whether or not this is contaminated [Re: Mr. Magic]
#21492587 - 04/02/15 02:22 PM (9 years, 1 month ago) |
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They are standard BRF PF tek jars, sterilized in pressure cooker for 70 minutes high pressure, inoculated 24 hours later under sterile conditions (ie: Inside sterilized inoculation chamber (lab quality) as well as including flame sterilization of the needle in between jars). incubating at 80*F for 13 days. All my other jars are doing phenomenal, however these ones were lagging at first and then started looking a little funky.
Edited by SporeWhore69 (04/02/15 02:23 PM)
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Mr. Magic



Registered: 07/13/14
Posts: 1,951
Last seen: 2 days, 15 hours
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Re: need help identifying whether or not this is contaminated [Re: SporeWhore69]
#21492818 - 04/02/15 03:17 PM (9 years, 1 month ago) |
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Might be bacterial maybe so the mycelium is having to compete, might be why they're coming along slower. 80F is a little warm but not bad either ive found around 70F to 75F works better, thats just my experience.
If your other jars are doing fine then just move those to a new location away from the others and see how they progress.
Bacteria strives in warmer conditions so keeping them closer to room temp is better imo. I have always had my jars fully colonized within a few weeks +half week or so while keeping them in that temp. Definately try not to let the temp climb too high.
Edited by Mr. Magic (04/02/15 03:21 PM)
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