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OfflineIsaiahkiki
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Registered: 10/16/14
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Liquid Culture Inoculation
    #21481001 - 03/30/15 08:38 PM (9 years, 1 month ago)

I have a question on LC inoculation. I was wondering if I can break off a peace of my PF cake and inoculate my Liquid cultures with it. It will be done with a glovebox and I was thinking of adding 1cc of h202 per 100ccs of water just to eliminate any contams on the cake. Sorry if this is already answered I looked everywhere

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InvisibleBig Bear
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Re: Liquid Culture Inoculation [Re: Isaiahkiki]
    #21481076 - 03/30/15 09:01 PM (9 years, 1 month ago)

you could potentially do it but it sounds risky.  the h2o2 is a bad idea and will not eliminate contams.  Your cake either is clean or isn't clean.  If you are going to do it do it like this guy does but replacing the grain with the liquid culture.
http://www.shroomery.org/forums/showflat.php?Cat=0&Number=20410651&page=0&vc=1#20410651


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Offlinemushpunx
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Re: Liquid Culture Inoculation [Re: Isaiahkiki]
    #21481089 - 03/30/15 09:05 PM (9 years, 1 month ago)

I wouldnt advise against this


Do your PF cakes have a dry verm layer? I cant think of good way to get under the dirty verm layer and breaking up the cake without touching it. Too impractical and too many vectors for contam

I would skip the LC all together until you can inoculate it with a clean culture from agar


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InvisibleBig Bear
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Re: Liquid Culture Inoculation [Re: mushpunx]
    #21481106 - 03/30/15 09:09 PM (9 years, 1 month ago)

yeah I should have mentioned it in my post, the only reason I recommended eat's method is because he uses SFD's on his lids.  Dry verm contam barrier for this project is definitely a no no imo


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Offlinetwistedty
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Re: Liquid Culture Inoculation [Re: Big Bear]
    #21481598 - 03/30/15 11:05 PM (9 years, 1 month ago)

AGAR.

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InvisibleBig Bear
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Re: Liquid Culture Inoculation [Re: twistedty]
    #21481743 - 03/30/15 11:58 PM (9 years, 1 month ago)

That too :lol:


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