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Offlineadsads
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Registered: 02/25/15
Posts: 18
Last seen: 8 years, 2 months
red spots on the back of agar plate
    #21463826 - 03/27/15 12:28 AM (9 years, 1 month ago)

hello guys.
i have inoculated my petri dishes with golden teacher spore syringe 7 days ago.now on the front everything looks white but at the back there are some reddish areas visible.what are these and what should be my next step????thanks a lot in advance







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InvisibleTheEaglesGift
The Nagual


Registered: 04/10/11
Posts: 10,554
Loc: Ixtlan, Mexico
Trusted Cultivator
Re: red spots on the back of agar plate [Re: adsads]
    #21463887 - 03/27/15 01:00 AM (9 years, 1 month ago)

I see nothing but clean growth.

Take a sample from the fastest leading edge of growth, and transfer to a new plate. The fastest and newest/freshest mycelium will be the mycelium that is furthest away from the point of inoculation(where you dropped your ms solution).

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InvisibleTheEaglesGift
The Nagual


Registered: 04/10/11
Posts: 10,554
Loc: Ixtlan, Mexico
Trusted Cultivator
Re: red spots on the back of agar plate [Re: TheEaglesGift]
    #21463888 - 03/27/15 01:01 AM (9 years, 1 month ago)

Kudos to you. First post and already sinking your teeth into agar work. :thumbup:

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Offlineadsads
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Re: red spots on the back of agar plate [Re: TheEaglesGift]
    #21465110 - 03/27/15 11:30 AM (9 years, 1 month ago)

thanks man.i m new to the shrooms world and chose to start with agar work.so as u said i will continue with strain isolation.by the way very useful insight on sector selection.thanks again

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InvisibleTheEaglesGift
The Nagual


Registered: 04/10/11
Posts: 10,554
Loc: Ixtlan, Mexico
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Re: red spots on the back of agar plate [Re: adsads]
    #21466615 - 03/27/15 05:10 PM (9 years, 1 month ago)

My pleasure. Let me know if you have any more questions. You can ask here, or the AMU thread in my signature.

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Offlineadsads
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Re: red spots on the back of agar plate [Re: TheEaglesGift]
    #21477969 - 03/30/15 09:16 AM (9 years, 1 month ago)

i have waited a couple of days and the red areas under the agar have increased. from the top mycelium is white but under the agar there are red spots.are these con tams or metabolites or what?medium is MEA.

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InvisibleTheEaglesGift
The Nagual


Registered: 04/10/11
Posts: 10,554
Loc: Ixtlan, Mexico
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Re: red spots on the back of agar plate [Re: adsads]
    #21479885 - 03/30/15 03:45 PM (9 years, 1 month ago)

Don't know. Transfer to a new plate?

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Offlineadsads
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Re: red spots on the back of agar plate [Re: TheEaglesGift]
    #21507043 - 04/05/15 05:17 PM (9 years, 1 month ago)

yes i have transferred to new plates and growing fine.how many transfers do i need before starting the spawning phase?i suppose reaching a mono culture is quit difficult

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InvisibleTheEaglesGift
The Nagual


Registered: 04/10/11
Posts: 10,554
Loc: Ixtlan, Mexico
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Re: red spots on the back of agar plate [Re: adsads]
    #21507296 - 04/05/15 06:26 PM (9 years, 1 month ago)

If your growth appears clean you can probably be safe to use it, but I'd do one more just to be safe.

You don't have to let it grow out so much before you make your transfer.

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Offlineadsads
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Re: red spots on the back of agar plate [Re: TheEaglesGift]
    #22175087 - 09/01/15 06:22 PM (8 years, 8 months ago)

Quote:

TheEaglesGift said:
If your growth appears clean you can probably be safe to use it, but I'd do one more just to be safe.

You don't have to let it grow out so much before you make your transfer.



thankss man.nice advice

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Offlinehightech
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Re: red spots on the back of agar plate [Re: adsads]
    #26555640 - 03/24/20 06:21 PM (4 years, 1 month ago)

Hi everyone, i am new to mushroom cultivation and it's my first time doing it and i would be greatful if you help me . I decided to go with agar. I prepared a storage place and inaculated my petri dishes with golden teacher  spores serynge. I might have put too much. I waned to be sure it works. So far it has been 1 week and i began to see red dots inside my petri dishes! May I ask if it is contaminated? I also made a mistake and had a flower light on on top of the dishes every day...


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Offlinerumfor69
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Re: red spots on the back of agar plate [Re: hightech]
    #26555783 - 03/24/20 07:43 PM (4 years, 1 month ago)

That looks like a plate full of bacteria


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OfflineSynKyd
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Re: red spots on the back of agar plate [Re: rumfor69]
    #26556082 - 03/24/20 10:20 PM (4 years, 1 month ago)

:whathesaid:

:dead:


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Offlinehightech
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Re: red spots on the back of agar plate [Re: SynKyd]
    #26556112 - 03/24/20 10:42 PM (4 years, 1 month ago)

Damn, really???? My spores are bad then! I thought those other things are okie :frown:

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Offlinerumfor69
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Re: red spots on the back of agar plate [Re: hightech]
    #26556164 - 03/24/20 11:28 PM (4 years, 1 month ago)

All those white blobs are bacteria.

Doesn't mean your spores are bad necessarily

but that dish is and you need to make another dish

and can try again with the same spore syringe or print


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Offlinehightech
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Re: red spots on the back of agar plate [Re: rumfor69]
    #26557767 - 03/25/20 08:02 PM (4 years, 1 month ago)

Thank you very much for taking the time to reply me. I still have some left .( like probably couple of cc) i used a presto pressure cooker to sterilze the agar solution. It doesn't have a pressure meter. May I ask if you have any recommendations on how hot the oven should be ? Or for how long should i let it be in there? And also how much water should be inside the pressure cooker ? One last question, may i ask how many drops is enough per dish? And that Can i just use electric tape to tape them seal after dropping the spores on them and then just store them without worrying for contamination ( since it's taped) .
Thx very much again! I really want to make this work...

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Offlinerumfor69
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Re: red spots on the back of agar plate [Re: hightech] * 1
    #26557956 - 03/25/20 09:59 PM (4 years, 1 month ago)

As long as the jiggler weight is rocking back and forth slightly it is at
15psi. You can turn down the heat in small increments at a time once it
starts rocking back and forth long as it stays rocking slightly at least.

If you are just putting individual plates of agar in the pressure cooker
they only need to go 15-20mins @15psi if it's a bottle of agar that you're
going to pour into a bunch of plates it should go 30mins @15psi

One quart of water will be ok for 15-30mins of cook time. Anything longer
use two quarts of water.

Any kind of tape you have will do in a pinch but I reccomend using 3M Micropore
tape. You only need to use a drop of spore solution. On plates the size
of yours I would use 3 drops in 3 different locations on each dish
If you're doing all this work in a SAB it's possible the contam was
in the air or your technique in hand movements caused it. Keep practicing
and keep dripping, all it has to do is be right once. Flame sterilize that
needle and let a few drops come out before dripping on a dish.


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Offlinehightech
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Re: red spots on the back of agar plate [Re: rumfor69]
    #26565302 - 03/29/20 01:21 PM (4 years, 1 month ago)

Thank you very much for taking the time to answer my questions in details. I did as you advised but i could not find the tape so i used the electric tape. This time I sprayed the air all around me with alcohol before puring the agar (inside Still air box) into the petri dishes. Imagine doing that with current condition that is impossible to find alchohol lol then waited for it to cool down enough and then inaculated them and taped them.
I inaculated 7 petri dishes. 4 i keep inside a sanitized plastic box and 3 are just on the shell in closet. I have also put a none-inaculated petri dish with them. If anything grows on it, it means that my method was wrong but if not then most probably the spores are contaminated. I did it 3 days ago and here is the result. May I ask if it looks any good? Is this bacterial growth again? The none inaculated petri dish is all fine...

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Invisiblealaskappalachian
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Registered: 10/22/19
Posts: 1,870
Loc: The 49th Dimension
Re: red spots on the back of agar plate [Re: hightech]
    #26565310 - 03/29/20 01:29 PM (4 years, 1 month ago)

Bacterial madness unfortunately.  When you do your next ones, make sure you use a single, tiny drop of spore solution.  Just one.  Tiny.  Do that in two or three places on a plate evenly spaced.  Often when you are working with a dirty ass spore syringe on agar, too much solution just creates the perfect little environment for the bacteria in the syringe to take off, which will sometimes completely retard the growth of any mycelium.  If you have trouble getting a drop out and not spurting it everywhere, just put a little pressure on the plunger where there is a drop hanging by surface tension, and touch it to the agar.  Try it real gently before you hit the plate with it and get your one-drop technique down.  It's not easy for some people.  Best of luck.  Keep on tikcing away at it.


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Offlinerumfor69
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Re: red spots on the back of agar plate [Re: alaskappalachian]
    #26565352 - 03/29/20 01:53 PM (4 years, 1 month ago)

Thats bacterial contamination that plate is no good.

Yeah all spores are contaminated to some extent it's just their nature.

Just have to keep trying the single drops like he said accross lots of

different plates until you get lucky


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