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Offlinednllln
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Registered: 02/03/15
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Syringe to Agar = Trich!
    #21457729 - 03/25/15 09:35 PM (9 years, 1 month ago)

Well, after the first syringe of two I bought from a sponsor contaminated 6/10 jars and the second syringe contaminated 5/5 jars (all with trich and one with cobweb) I decided to whip up some agar plates. I made a total of 6 agar plates to start with. Shook the syringe and flame sterilized between each plate, cooled the syringe in the SAB, and then placed one drop per plate.

A week later every plate grew nothing except this one! At least I succeeded in germinating SOMETHING. Standard LME formula. Maybe I'm not giving it long enough for the other plates, but damn. It's kinda discouraging.

Either way, I threw out the rest of that particular syringe. I just knocked up another 5 BRF jars from a GT syringe from another sponsor. We'll see how that one goes!

It's hard to see because of my camera but it's a nice deep forest green. Too bad growing trich isn't my goal. I bet I could get a nice little colony going.

I poured a bit too much agar in the wrong type of jar as well, haha. Waiting on my 8oz jars still.

Any recommendations? Should I attempt to isolate and create a trich monoculture? :rolleyes:


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OfflinecronicrFacebook
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Re: Syringe to Agar = Trich! [Re: dnllln]
    #21457737 - 03/25/15 09:38 PM (9 years, 1 month ago)

just order prints instead of syringes:shrug: way more worth it bro


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Offlinednllln
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Registered: 02/03/15
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Re: Syringe to Agar = Trich! [Re: cronicr]
    #21457744 - 03/25/15 09:40 PM (9 years, 1 month ago)

I've already got about 8 cakes fruiting from my very first batch. None of those contaminated, though two didn't grow anything. I'm planning on cloning and taking some prints from that batch! This was a mazatapec strain I was going to try to save, but alas, no germination.

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InvisibleUncleFester
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Registered: 09/23/14
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Re: Syringe to Agar = Trich! [Re: cronicr]
    #21457747 - 03/25/15 09:41 PM (9 years, 1 month ago)

How are spore prints better? Is it because you can make more syringes?


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InvisibleK1ngSp4de
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Registered: 01/16/12
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Re: Syringe to Agar = Trich! [Re: UncleFester]
    #21457752 - 03/25/15 09:43 PM (9 years, 1 month ago)

No you go straight from print to agar with an inoculation loop.


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OfflinecronicrFacebook
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Re: Syringe to Agar = Trich! [Re: K1ngSp4de]
    #21457758 - 03/25/15 09:45 PM (9 years, 1 month ago)

:whathesaid: he's already playing with agar....one print could last longer then a life time:hehehe:


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InvisiblePirateSwazey
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Re: Syringe to Agar = Trich! [Re: cronicr]
    #21457792 - 03/25/15 09:55 PM (9 years, 1 month ago)

If you get some mushies from your cakes you can try some cloning..

Just take a little piece of tissue from inside the stem & put it on agar.

Transfer once or twice then inoculate grain.

You can keep transferring to isolate it too if you want.

Quote:

cronicr said:
'It doesn't matter what I think of you..all that matters is clean spawn'



:whathesaid: You're on the way OP :thumbup:


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