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Offlinegreendaguilera
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Registered: 09/07/13
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Last seen: 7 years, 9 months
Stupid question
    #21455904 - 03/25/15 02:36 PM (9 years, 1 month ago)

Hi.
Ok, so I've heard g2g only works for a few generations. So my dumb question is this;

If I used a mushroom made by a g2g to make a clone with agar instead of using spores would that work? Some mushrooms are hard to get spores from.

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Offlinespacechildo
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Registered: 01/24/13
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Re: Stupid question [Re: greendaguilera]
    #21455924 - 03/25/15 02:43 PM (9 years, 1 month ago)

yes that would work. spores - (agar) - grain - g2g - bulk - mushroom - agar?
I think I understood your question... hmm.

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Invisibleswatt_haze
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Registered: 11/06/14
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Re: Stupid question [Re: spacechildo]
    #21455943 - 03/25/15 02:47 PM (9 years, 1 month ago)

Idk.

RR-"
  *Four grain-to-grain transfers should be considered the outside limit, but chances are you'll see growth slow before then anyway. Cloning from the third would make it the fourth, and wouldn't help. Go back to spores. A given cell line can only divide so many times before loss of vigor sets in. G2G TRANSFERS"

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Invisibleswatt_haze
Mkay
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Re: Stupid question [Re: swatt_haze]
    #21455949 - 03/25/15 02:48 PM (9 years, 1 month ago)

Also,

RR- *It was ANNO that pointed out - young vigorous mycelium does better than old. However, I have gone out 5 G2G transfer generations - without ill effect. Genetics plays a part in it. In nature - mycelium is designed to grow out & fruit in one season. You can FOOL with Mother Nature - but extending it to far out - usually brings on some mutation. G2G GENERATIONS

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OfflinecronicrFacebook
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Registered: 08/07/11
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Re: Stupid question [Re: swatt_haze]
    #21455953 - 03/25/15 02:49 PM (9 years, 1 month ago)

:whathesaid:
yes that would work but may not be ideal depending on how many g2g's ya did, a couple would be fine though but for storing purposes you want to limit the cell division so there cool for a quick grow or two but for long term your best bet is a young culture


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Offlinegreendaguilera
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Registered: 09/07/13
Posts: 10
Last seen: 7 years, 9 months
Re: Stupid question [Re: swatt_haze]
    #21455955 - 03/25/15 02:50 PM (9 years, 1 month ago)

Damn. That answers my question. Thanks.

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