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TheEaglesGift
The Nagual


Registered: 04/10/11
Posts: 10,554
Loc: Ixtlan, Mexico
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Quote:
NumeroEno said:
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TheEaglesGift said:
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By the way, that agar you are using is just about as dark as the agar recipe I am working on. Is that just grain water that you are using that makes it that dark or are you using something else?
I use powdered dark malt extract instead of light malt extract. It was cheaper.
I get a pound of GLME for 5 bucks and change at the brewing supply store down the street. Any noticeable difference between dark and light ME in terms of how the mushrooms like it?
I don't notice a difference wither way. Malt extract is malt extract, as far as I can tell.
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agreedwitboardrule
StrangeStranger
Registered: 12/04/13
Posts: 317
Loc: Asia
Last seen: 5 years, 10 months
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How long approx will it take the myc to start growing on the agar?
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insanemike

Registered: 02/23/14
Posts: 4,272
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Quote:
agreedwitboardrule said: How long approx will it take the myc to start growing on the agar?
Depends on the method of inoculation.
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agreedwitboardrule
StrangeStranger
Registered: 12/04/13
Posts: 317
Loc: Asia
Last seen: 5 years, 10 months
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Quote:
insanemike said:
Quote:
agreedwitboardrule said: How long approx will it take the myc to start growing on the agar?
Depends on the method of inoculation.
Injecting spores with a syringe....as per the tek.
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MudaFuka
Poppin bottles



Registered: 12/14/13
Posts: 18,648
Loc: Canada
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I generally see growth in 3 to 7 days but it can take a couple weeks.
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TheEaglesGift
The Nagual


Registered: 04/10/11
Posts: 10,554
Loc: Ixtlan, Mexico
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Spore germination on agar takes the same general amount of time as spore germination on brf/grain. So, anywhere from 5ish-15ish days.
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TheEaglesGift
The Nagual


Registered: 04/10/11
Posts: 10,554
Loc: Ixtlan, Mexico
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If you're germinating spores on agar, make a softer agar by adding less agar to your recipe. Spores germinate on softer agar easier.
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agreedwitboardrule
StrangeStranger
Registered: 12/04/13
Posts: 317
Loc: Asia
Last seen: 5 years, 10 months
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I wanted to ask - why is the condensation such a big issue? contams?
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agreedwitboardrule
StrangeStranger
Registered: 12/04/13
Posts: 317
Loc: Asia
Last seen: 5 years, 10 months
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Quote:
TheEaglesGift said: If you're germinating spores on agar, make a softer agar by adding less agar to your recipe. Spores germinate on softer agar easier.
and if my agar isn't soft at all?....if it's quite hard as i was enthuastic about adding agar? will it still work?
-------------------- I like you.
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hamloaf
Pork Block


Registered: 12/23/09
Posts: 20,452
Loc: Oklahoma.
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Quote:
agreedwitboardrule said: I wanted to ask - why is the condensation such a big issue? contams?
Condensation in agar media vessels can be a vector for contam to the mush culture running on the agar. Another major foul created by condensation generated inside of your agar media vessels is not being able to see culture performance through media vessel.
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Grey
⇜ ✯ ⇝



Registered: 11/06/14
Posts: 6,223
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Quote:
agreedwitboardrule said:
Quote:
TheEaglesGift said: If you're germinating spores on agar, make a softer agar by adding less agar to your recipe. Spores germinate on softer agar easier.
and if my agar isn't soft at all?....if it's quite hard as i was enthuastic about adding agar? will it still work?
I've had some plates, when playing with the recipe, turn out very firm like dried candle wax. It still worked out for me.
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AMU Q&A If you don't have a plan of your own, you'll become a part of somebody else's.
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agreedwitboardrule
StrangeStranger
Registered: 12/04/13
Posts: 317
Loc: Asia
Last seen: 5 years, 10 months
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Re: Pasty Agar Tek [Re: Grey]
#21438635 - 03/21/15 08:19 AM (8 years, 10 months ago) |
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Quote:
Grey said:
Quote:
agreedwitboardrule said:
Quote:
TheEaglesGift said: If you're germinating spores on agar, make a softer agar by adding less agar to your recipe. Spores germinate on softer agar easier.
and if my agar isn't soft at all?....if it's quite hard as i was enthuastic about adding agar? will it still work?
I've had some plates, when playing with the recipe, turn out very firm like dried candle wax. It still worked out for me.
Ok Cool!
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TheEaglesGift
The Nagual


Registered: 04/10/11
Posts: 10,554
Loc: Ixtlan, Mexico
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Softer agar for spore germination. Stiffer agar for transfer work.
This is a generality, either agar stiffness will work for either application, even if it's not ideal.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,890
Loc: Milky way
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15-20g/L is an acceptable range for food grade agar dish making.
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transkei
Mr



Registered: 05/24/07
Posts: 218
Loc: Texas
Last seen: 1 year, 11 months
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Re: Pasty Agar Tek *DELETED* [Re: bodhisatta]
#21453559 - 03/24/15 10:43 PM (8 years, 10 months ago) |
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Post deleted by transkeiReason for deletion: answer was deleted
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Edmunter
Mr



Registered: 05/01/13
Posts: 5,699
Last seen: 1 month, 12 days
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Re: Pasty Agar Tek [Re: transkei]
#21454344 - 03/25/15 05:22 AM (8 years, 10 months ago) |
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Can anyone tell what sectors to go for or should I do this elsewhere Pasty?
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,811
Loc: Canada
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Re: Pasty Agar Tek [Re: Edmunter]
#21454346 - 03/25/15 05:24 AM (8 years, 10 months ago) |
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It's not really sectoring yet but the growth at 2 o'clock looks fastest
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blindingleaf
blue collar underworld


Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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Re: Pasty Agar Tek [Re: transkei]
#21454351 - 03/25/15 05:27 AM (8 years, 10 months ago) |
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Quote:
transkei said: going to add a little yeast to my formula 1 cup water, 5grams agar,6.6grams potato flakes,1/4 tsp karo. can I use one of these? which is best and with that formula how much? I am in a hurry, if not I would do a search. Thanks in advance
either one will work, but i'd go with red one
i use 1g/500ml h2o, so for ur recipe use .5g per cup h2o
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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Edmunter
Mr



Registered: 05/01/13
Posts: 5,699
Last seen: 1 month, 12 days
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Re: Pasty Agar Tek [Re: Edmunter]
#21454419 - 03/25/15 06:16 AM (8 years, 10 months ago) |
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Quote:
Edmunter said: Can anyone tell what sectors to go for or should I do this elsewhere Pasty?

This was grown on Grain water and agar. Its really happy.
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Edmunter
Mr



Registered: 05/01/13
Posts: 5,699
Last seen: 1 month, 12 days
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Re: Pasty Agar Tek [Re: Edmunter]
#21454422 - 03/25/15 06:18 AM (8 years, 10 months ago) |
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Quote:
Edmunter said: Can anyone tell what sectors to go for or should I do this elsewhere Pasty?

This was grown on Grain water and agar. Its really happy!
So a nice wedge from 2 oclock would be be one transfer. Anywhere else?
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