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OfflineMycologist217
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Re: Grow Log- 1st Bulk Attempt- WBS and Coir - Lab Manual Style- with pics [Re: twistedty]
    #21491737 - 04/02/15 10:22 AM (9 years, 9 months ago)

Day 9-10
It's approximately 10 days post inoculation maybe 9.5 but almost all jars are completely over taken by mycelium. I only shook the jars on day 4 and then on day 8. It was indicated to me by another poster that this fast of colonization could mean contamination? Don't understand why...,the dispersal of the spores across the jar into what could be dozens to hundreds of points of germination might have just led to an exponentially shorter colonization time? Not to mention constant incubation at 80 degrees... Here's a 5 jar sample from last night


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Offlinetwistedty
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Re: Grow Log- 1st Bulk Attempt- WBS and Coir - Lab Manual Style- with pics [Re: Mycologist217]
    #21491913 - 04/02/15 11:09 AM (9 years, 9 months ago)

love them after post shake :thumbup:

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Invisiblefilthyknees
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Re: Grow Log- 1st Bulk Attempt- WBS and Coir - Lab Manual Style- with pics [Re: Mycologist217]
    #21491968 - 04/02/15 11:24 AM (9 years, 9 months ago)

I would be skeptical of 100% jars in 10 days from spore inoculation. It takes days like half that time for the spores to even start myc.
Along with that patchy growth (if those pictures you posted are supposed to be 100% cube myc) and saying you incubate at 80 degrees I would say those look suspect.


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InvisibleMad Season
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Re: Grow Log- 1st Bulk Attempt- WBS and Coir - Lab Manual Style- with pics [Re: filthyknees]
    #21492239 - 04/02/15 12:37 PM (9 years, 9 months ago)

They look kinda off. If they look 100% give em a wiff and see what they smell like


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OfflineMycologist217
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Re: Grow Log- 1st Bulk Attempt- WBS and Coir - Lab Manual Style- with pics [Re: Mad Season]
    #21492489 - 04/02/15 02:01 PM (9 years, 9 months ago)

They don't look 100% but easily close to it. I did open one and smell it...it smelled like wild bird seed and mushroom...not even remotely foul. This log documents that mycelium appeared on day 3.....I don't think there is anything strange about that. What about the, looks off? My PF TEK jars colonized 100% in just 15 days and they don't get mixed up....


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OfflineMycologist217
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Re: Grow Log- 1st Bulk Attempt- WBS and Coir - Lab Manual Style- with pics [Re: Mycologist217]
    #21492556 - 04/02/15 02:14 PM (9 years, 9 months ago)

The docs wbs tek specifically says to incubate at 80 degrees...its apparent to me that the 80 degree incubation is what causes the faster growth...been incubating 31 pf jars at 80 degrees for the last 22 days. I inoculated 36 jars total but 5 of them didn't show mycelium at 1/4 inoculation points a week or so after inoculation so I prematurely threw them out. They were not visually contaminated

As far as my wbs jars go...i feel like the fact that they are all growing at similar pace is a good thing...meanwhile not a single one shows a visual
of being contaminated and the one I sniffed smelled absolutely pleasant.


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Edited by Mycologist217 (04/02/15 02:19 PM)

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InvisibleMad Season
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Re: Grow Log- 1st Bulk Attempt- WBS and Coir - Lab Manual Style- with pics [Re: Mycologist217]
    #21493242 - 04/02/15 05:23 PM (9 years, 9 months ago)

I don't incubate. My jars finished in 10 days at 70-75 temps I wouldn't throw jars that show nothing after  a week out.. I've had jars take 4 weeks just for the spores to germinate


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OfflineCaptainpaps
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Re: Grow Log- 1st Bulk Attempt- WBS and Coir - Lab Manual Style- with pics [Re: Mad Season]
    #21494535 - 04/02/15 09:58 PM (9 years, 9 months ago)

give em all a good shake and if things are good they should recover in about 42 hours


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OfflineMycologist217
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Re: Grow Log- 1st Bulk Attempt- WBS and Coir - Lab Manual Style- with pics [Re: Mycologist217]
    #21494556 - 04/02/15 10:02 PM (9 years, 9 months ago)

Quote:

Mycologist217 said:
Day 9-10
It's approximately 10 days post inoculation maybe 9.5 but almost all jars are completely over taken by mycelium. I only shook the jars on day 4 and then on day 8.




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InvisibleMad Season
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Re: Grow Log- 1st Bulk Attempt- WBS and Coir - Lab Manual Style- with pics [Re: Mycologist217]
    #21496018 - 04/03/15 10:55 AM (9 years, 9 months ago)

They sound good man nothing to worry about. If they smell fine they are fine. The pics are just bad angles or just a good example of multispore. Just keep doing what you're doing.

Were just saying the incubator isn't necessary. You'll see lots of condensation on the walls of the jars because the mycelium will heat up as its colonizing and make the internal temp of the jar above 80 while in a room temp of 65-75 according to a post from RR I read long ago


--------------------
contam and car window art
How to shroomery like a pro! (Seriously, everyone read this!)
Improve your sterile techniques! (A comprehensive guide to agar)
Links upon links of literally EVERYTHING UP TO DATE

AMU Q&A
No trees were killed in the sending of this message. However, a large number of electrons were terribly inconvenienced.

Edited by Mad Season (04/03/15 10:55 AM)

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Invisiblenobody83
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Re: Grow Log- 1st Bulk Attempt- WBS and Coir - Lab Manual Style- with pics [Re: Mad Season]
    #21496097 - 04/03/15 11:10 AM (9 years, 9 months ago)

:popcorn:


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OfflineMycologist217
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Re: Grow Log- 1st Bulk Attempt- WBS and Coir - Lab Manual Style- with pics [Re: nobody83]
    #21504554 - 04/04/15 11:43 PM (9 years, 9 months ago)

Day 12-13
I've begun pasteurization of my coir substrate tonight. I used 1 brick of coir, 2 quarts of course vermiculite, and a handful of gypsum. I'm about to hydrate my spawn as per franks spawning TEK.


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Invisiblenobody83
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Re: Grow Log- 1st Bulk Attempt- WBS and Coir - Lab Manual Style- with pics [Re: Mycologist217]
    #21504564 - 04/04/15 11:45 PM (9 years, 9 months ago)

:thumbup:


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OfflineMycologist217
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Re: Grow Log- 1st Bulk Attempt- WBS and Coir - Lab Manual Style- with pics [Re: nobody83]
    #21504847 - 04/05/15 02:11 AM (9 years, 9 months ago)

Day 13-
Spawned my WBS tonight using six fully colonized quarts. I gave each jar a thorough sniff and was delighted to smell nothing but fresh mushroom. I cannot express how nervous I am now. The bucket TEK isn't exactly full proof and my FAE holes didn't exactly land at substrate level....but they are no more than an inch above it.

I dunked the jars for a good a 17 minutes before spawning as per franks TEK. I know adding nutrient like gypsum is damn near inviting contam, but his TEK says that if the coir is properly Pasteurized and the spawn is 100% colonized, then I will not see a contamination before flush one. Obviously I'm hoping for a speedy colonization. Curious minds can observe my...okay...first attemp at constructing a Monotub. I really hope this works out. Let me know how you think the construct looks.


At any rate, I have 9-18 PF TEK jars hitting the dunk and roll tomorrow. So there are fruitbodies in my future. Wish me luck nonetheless


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OfflineSockadin
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Re: Grow Log- 1st Bulk Attempt- WBS and Coir - Lab Manual Style- with pics [Re: Mycologist217]
    #21504953 - 04/05/15 03:42 AM (9 years, 9 months ago)

:thumbup:

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OfflineMycologist217
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Re: Grow Log- 1st Bulk Attempt- WBS and Coir - Lab Manual Style- with pics [Re: Sockadin]
    #21509871 - 04/06/15 11:26 AM (9 years, 9 months ago)

Day 15-
It's approximatel a day and a half since I spawned 6quarts of WBS to my first constructed Monotub. I spawned the 6 quarts to a coir/Verm/gypsum/water substrate.  Only 36 hours later I see tremendous progress. This is what my tub looks like today:


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OfflineMycologist217
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Re: Grow Log- 1st Bulk Attempt- WBS and Coir - Lab Manual Style- with pics [Re: Mycologist217]
    #21514998 - 04/07/15 01:11 PM (9 years, 9 months ago)

Day 16-
The Monotub seems to still be colonizing very nicely. I can see the rhizomorphic branching of the mycelium across the surface. It's really cool to observe that in some places it is just starting to be taken by the mycelium where others have dense ropey growth completely whiting out the substrate.  There is light condensation on the walls of the Monotub and the lid is regularly obscured with fog, like breathing directly on a window pane. Super excited about the next week or so. The condensation made it tough to get a decent shot of the tub today, but I can tell everything looks great...with one exception. I didn't really work hard to level out my tub.,,it's actually pretty bumpy...,is leveling it out super important?

Edited by Mycologist217 (04/07/15 01:12 PM)

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Invisiblenobody83
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Re: Grow Log- 1st Bulk Attempt- WBS and Coir - Lab Manual Style- with pics [Re: Mycologist217]
    #21519094 - 04/08/15 11:24 AM (9 years, 9 months ago)

Nice work:thumbup: just for kicks I would make hills and troughs in my tubs,worked out great


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Re: Grow Log- 1st Bulk Attempt- WBS and Coir - Lab Manual Style- with pics [Re: nobody83]
    #21519815 - 04/08/15 03:02 PM (9 years, 9 months ago)



--------------------
But if you're in a hurry, and really got to go
If you're in a hurry, might have to find out slow
That it's one thing to try and another to fly
You get there quicker just a step at a time
It's one thing to bark, another to bite
The show ain't over till you pack up at night

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OfflineMycologist217
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Re: Grow Log- 1st Bulk Attempt- WBS and Coir - Lab Manual Style- with pics [Re: filthyknees]
    #21520277 - 04/08/15 05:06 PM (9 years, 9 months ago)

Day 17-
Montub has been colonizing since late Saturday night. It is getting so covered I. White rhizomorphic mycelium that they yellow specs of bird seed at starting to stand out. It's awesome see the myc reaching over and grabbing the substrate. Can't wait to see this to full colonization and introduce it to fruiting conditions. I hope my first bulk pin set is beautiful, but I have to keep some modesty about it...still have a long way to go before a healthy first harvest. At any rate here's a picture through the lid of the Monotub....the darker area is just in shadow:

Let me know if you have concerns or questions


--------------------
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Mycologist217 is a fictitious entity that uses images supplied by Google to mask his/her inability to develop normal social habits.

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