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OfflineUrist
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Registered: 02/11/15
Posts: 372
Loc: A Dwarf Fortress
Last seen: 9 months, 2 days
Do these agar plates look alright to you guys?
    #21434423 - 03/20/15 02:20 AM (9 years, 1 month ago)

This is my first attempt at agar or mushroom cultivation in general (I live in Cali, so no spores) so the agar is poured a little thick and there's some condensation on the lids. These pics are about a week after putting the tissue on the agar.

Also sorry for poor quality...the condensation fucks up the focus




Even I can see this one is clearly fucked. Aspergillium?






P. Cubensis. Very cotton-like growth, Cobweb or fuzzy mycelium?






P. Cubensis. Also cotton-like growth but not nearly as fuzzy.






P. Cubensis. This is the best-looking one so far, but I'm new so it could just be really healthy cobweb...






Pleurotus Ostreatus. No visible growth other than that yellowish part that looks possibly bacterial






Pleurotus Ostreatus. Visible white growth, possibly mycelium? Also more bacteria.



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OfflinecronicrFacebook
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Registered: 08/07/11
Posts: 61,436
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Re: Do these agar plates look alright to you guys? [Re: Urist] * 1
    #21434430 - 03/20/15 02:26 AM (9 years, 1 month ago)

pics 23 and 4 look like myc and that last pic looks like oysters to me


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OfflineUrist
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Registered: 02/11/15
Posts: 372
Loc: A Dwarf Fortress
Last seen: 9 months, 2 days
Re: Do these agar plates look alright to you guys? [Re: cronicr]
    #21434449 - 03/20/15 02:34 AM (9 years, 1 month ago)

I wasn't expecting that many to make it with such minimal contamination...I don't have enough dishes to transfer all of them, which of the Cubensis cultures should I transfer? or do you think they're clean enough to inoculate directly?

Also when I DO inoculate, should I drop the agar wedge in directly or should I make a LC first?


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InvisibleBuck513

Registered: 04/17/14
Posts: 5,682
Re: Do these agar plates look alright to you guys? [Re: Urist] * 1
    #21434471 - 03/20/15 02:45 AM (9 years, 1 month ago)

I'd just drop a wedge in some grain and shake it after inoc.

If I were you, I would take a couple wedges from pic4 and see which transfer does best. I'd also get a wedge from pic 2


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Invisibleswatt_haze
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Registered: 11/06/14
Posts: 491
Re: Do these agar plates look alright to you guys? [Re: Buck513] * 1
    #21434618 - 03/20/15 05:50 AM (9 years, 1 month ago)

off topic - cronic always changing avatar and now u steal franks ? dun dun dun :stoned: i like the blonde with the titties :rockon:, im a little high and u just confused me, i thought u were frank... stop freakin me out:stoner:

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