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Urist
The Dwarf



Registered: 02/11/15
Posts: 372
Loc: A Dwarf Fortress
Last seen: 9 months, 2 days
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Do these agar plates look alright to you guys?
#21434423 - 03/20/15 02:20 AM (9 years, 1 month ago) |
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This is my first attempt at agar or mushroom cultivation in general (I live in Cali, so no spores) so the agar is poured a little thick and there's some condensation on the lids. These pics are about a week after putting the tissue on the agar.
Also sorry for poor quality...the condensation fucks up the focus
Even I can see this one is clearly fucked. Aspergillium?

P. Cubensis. Very cotton-like growth, Cobweb or fuzzy mycelium?

P. Cubensis. Also cotton-like growth but not nearly as fuzzy.

P. Cubensis. This is the best-looking one so far, but I'm new so it could just be really healthy cobweb...

Pleurotus Ostreatus. No visible growth other than that yellowish part that looks possibly bacterial

Pleurotus Ostreatus. Visible white growth, possibly mycelium? Also more bacteria.
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 3 months
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Re: Do these agar plates look alright to you guys? [Re: Urist] 1
#21434430 - 03/20/15 02:26 AM (9 years, 1 month ago) |
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pics 23 and 4 look like myc and that last pic looks like oysters to me
--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Urist
The Dwarf



Registered: 02/11/15
Posts: 372
Loc: A Dwarf Fortress
Last seen: 9 months, 2 days
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Re: Do these agar plates look alright to you guys? [Re: cronicr]
#21434449 - 03/20/15 02:34 AM (9 years, 1 month ago) |
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I wasn't expecting that many to make it with such minimal contamination...I don't have enough dishes to transfer all of them, which of the Cubensis cultures should I transfer? or do you think they're clean enough to inoculate directly?
Also when I DO inoculate, should I drop the agar wedge in directly or should I make a LC first?
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Buck513

Registered: 04/17/14
Posts: 5,682
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Re: Do these agar plates look alright to you guys? [Re: Urist] 1
#21434471 - 03/20/15 02:45 AM (9 years, 1 month ago) |
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I'd just drop a wedge in some grain and shake it after inoc.
If I were you, I would take a couple wedges from pic4 and see which transfer does best. I'd also get a wedge from pic 2
-------------------- Fail to plan and you plan to fail. Enter the Ban Lottery
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swatt_haze
Mkay



Registered: 11/06/14
Posts: 491
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Re: Do these agar plates look alright to you guys? [Re: Buck513] 1
#21434618 - 03/20/15 05:50 AM (9 years, 1 month ago) |
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off topic - cronic always changing avatar and now u steal franks ? dun dun dun i like the blonde with the titties , im a little high and u just confused me, i thought u were frank... stop freakin me out
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