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Offlineeltrkbrd
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Registered: 02/23/07
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Re: Invitro Grass Seed TEK – A powerful single-step grow method for beginners and beyond [Re: Violet]
    #21425750 - 03/18/15 08:19 AM (9 years, 2 months ago)

Has anyone tried this method using a Panaeolus Cambodginiensis (Sandose) multispore syringe to inoculate with a CMC based substrate?


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My way of giving back- Thanks Shroomery...
A For Newbs by Newbs Cultivation Resource Journal: http://www.shroomery.org/forums/showflat.php/Cat/0/Number/6748262/an/0/page/0

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InvisibleViolet
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Re: Invitro Grass Seed TEK – A powerful single-step grow method for beginners and beyond [Re: eltrkbrd]
    #21425794 - 03/18/15 08:36 AM (9 years, 2 months ago)

I highly doubt it.  Especially considering the method is fairly new.
It's also designed for Cubensis.  It may very well work with some exotic species, but those that are picky for lots of fresh air may not have ideal growth patterns in these enclosed containers.  They are not as fresh air problematic as many bulk substrate methods, and so it definitely works without necessarily having fresh air problems, but Cubensis is notably more tolerant of CO2 buildup than most other species.
I would think it would be nifty to see some attempts with Panaeolus for this method. It may have a better chance for Galindoi, Tampanensis, and Mexicana species though.


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Intentionally or not, here in mushcult we are purveyors of love culture and enlightenment movement. Let's try to act like it!

PODS TEK - Growing Invitro with BRF/verm or Grass Seed containers
The simplest, quickest, safest tek!  For beginners, culturers, lazy people, stealth lovers, contam haters, and alternative seekers!
Violet's Teks and Posts

Edited by Violet (03/18/15 01:44 PM)

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OfflineJerome09
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Re: Invitro Grass Seed TEK – A powerful single-step grow method for beginners and beyond [Re: eltrkbrd]
    #21426552 - 03/18/15 12:18 PM (9 years, 2 months ago)

hmm.  Could you use these 32oz plastic jars as fruiting chambers for pf cakes?  Birth, dunk and roll and then put in the 32oz ziploc?


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InvisibleViolet
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Re: Invitro Grass Seed TEK – A powerful single-step grow method for beginners and beyond [Re: Jerome09]
    #21426922 - 03/18/15 01:44 PM (9 years, 2 months ago)

I guess so.  Might as well sterilize the sub in the container and inoculate it so that they likely colonize more quickly and the cakes fit to the container nicely.


--------------------
Intentionally or not, here in mushcult we are purveyors of love culture and enlightenment movement. Let's try to act like it!

PODS TEK - Growing Invitro with BRF/verm or Grass Seed containers
The simplest, quickest, safest tek!  For beginners, culturers, lazy people, stealth lovers, contam haters, and alternative seekers!
Violet's Teks and Posts

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Offlineinvitro

Registered: 05/03/13
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Re: Invitro BRF or Grass Seed TEK – A powerful single-stage grow method for beginners and beyond [Re: Violet]
    #21427039 - 03/18/15 02:23 PM (9 years, 2 months ago)

:takingnotes:

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OfflineMushroom_J
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Re: Invitro Grass Seed TEK – A powerful single-step grow method for beginners and beyond [Re: Jerome09] * 1
    #21427089 - 03/18/15 02:35 PM (9 years, 2 months ago)

Quote:

Jerome09 said:
hmm.  Could you use these 32oz plastic jars as fruiting chambers for pf cakes?  Birth, dunk and roll and then put in the 32oz ziploc?




Flip the container upside down. put the cake on the lid and set the container over that leaving a crack for air.


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OfflineMBabble
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Re: Invitro Grass Seed TEK – A powerful single-step grow method for beginners and beyond [Re: Mushroom_J]
    #21438579 - 03/21/15 07:37 AM (9 years, 1 month ago)

Violet, have you worked at all with wild rice?
It's actually a grass seed is why I asked.

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InvisibleViolet
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Re: Invitro Grass Seed TEK – A powerful single-step grow method for beginners and beyond [Re: MBabble]
    #21439312 - 03/21/15 12:24 PM (9 years, 1 month ago)

Well all grains are a grass seed one way or another. Actual grass seed as we call it is quite different from cereal grains though. Different enough to warrant calling one seed and the others grains, including rice, including wild rice.
I've used whole rice a lot.  I haven't seen a point in buying expensive wild rice for results that will be ultimately no different.


--------------------
Intentionally or not, here in mushcult we are purveyors of love culture and enlightenment movement. Let's try to act like it!

PODS TEK - Growing Invitro with BRF/verm or Grass Seed containers
The simplest, quickest, safest tek!  For beginners, culturers, lazy people, stealth lovers, contam haters, and alternative seekers!
Violet's Teks and Posts

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OfflineMushroom_J
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Re: Invitro Grass Seed TEK – A powerful single-step grow method for beginners and beyond [Re: Violet]
    #21470628 - 03/28/15 03:22 PM (9 years, 1 month ago)

Invitro BRF.
inoculated: Mazatapec- (3) 3/22/15 Orissa india- (1) 3/23/15

Signs of growth: maz1 3/26/15, maz2 3/27/15, maz3 3/27/15
Orissa india 3/28/15

4-5 days for germination at 70f.


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OfflineMushroom_J
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Re: Invitro Grass Seed TEK – A powerful single-step grow method for beginners and beyond [Re: Mushroom_J]
    #21495136 - 04/03/15 02:53 AM (9 years, 1 month ago)

4/3/15



Going a little slow


Shook this one up yesterday. The rest will go unshook.


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OfflineJerome09
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Re: Invitro BRF or Grass Seed TEK – A powerful single-stage grow method for beginners and beyond [Re: Violet] * 1
    #21541288 - 04/13/15 11:21 AM (9 years, 1 month ago)

Harvested my first invitro tek today...
I am pretty stoked.  love this grow method
THanks Violet :blush: 
Really nice to have an all in one system!!

This culture was Bon Hua Thanon MS to WBS. 

So actually, this was an experiment. I took this colonized WBS jar and split it into 3 invitro jars as a test.



The first one (pic on left) was just  straight WBS and cased it...
The second one (Middle pic) I did GTG with Whole Brown rice... Many pins growing.. awkardly 
And the last one was the WBSF cake I did gtg (right pic)

So, as you can see, the WBSF cake was WAY quicker to fruit. It also had more pins. Maybe just luck or how the ms culture responded to WBSF cake but the spawn came from the same jar... so not a bad experiment.

This WBSF cake also fruited before 2 RGS invitro cakes even though the RGS cakes were inoculated G2G a week before the WBSF cake was.

Like I said, nothing definitive, especially because I can't wait to see the power of straight RGS, but give WBSF cakes a try they might blow your mind with their speed and good flushes..

Here are some not so great pics today of the WBSF cake and the first flush laid out.  Not the biggest harvest anyone has seen but pretty good and vigorous especially for 1/4 cup WBSF and 1/2 cup Verm



Thanks be to Violet.. after a failed mini mono :confused: this feels really, really good :thumbup::thumbup::thumbup:.  And the modification to this tek is endless,, can't wait to start again


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OfflineLabmonkey43a
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Registered: 11/29/14
Posts: 6
Loc: WA, US
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Re: Invitro BRF or Grass Seed TEK – A powerful single-stage grow method for beginners and beyond [Re: Jerome09]
    #21562827 - 04/18/15 10:09 AM (9 years, 1 month ago)

Hi Everyone,

Noob here.  Well, sorta.  I should say that I'm a fantastic mycelium farmer.  I mean, I can grow some great stuff with pretty low contams (always know why if it occurs).  I just can't get anything to ever fruit.  <facepalm>  (except my oysters, those worked great)

I'm doing straight grass seed in all of these examples in a combination of glass quart (plastic lids) and ziploc quart containers.  Dedicated room is 70-75* with florescent light on them in a 12/12 cycle with little/no traffic.  I have about four various isolated strains (not spores).  Most of these were inoculated with agar, but I also have a few I inoculated with colonized WBS (this seemed a much better way than full monotub attempts which is what I was doing before).

Here are a couple of current examples.  In this photo, the left jar was inoculated on 4/3, the right on 3/24.  Both were cracked on 4/13.



I had quart jars before I tried the ziploc containers.  I have one that was inoculated on 3/22, opened on 4/10.  Same sort of results.  (I do love the mycelium highways on the glass)

If I open one of my ziploc containers in front of my hood, it looks like this:


I guess I don't know if I'm casing it too long or not long enough.  Am I not being patient enough?  Too wet, too dry?  I've been sorta at this since about December and have yet to see fruit trying various methods, so I'm doing something terribly wrong. :laugh:

I made some of the POM-tek jars (with the seed drain and some agar) also in the hopes that the nutrient depletion on the pom would help in the process (but they're not fully colonized yet).

Any tips or ideas?  :smile:  Happy to provide more photos or answer to questions on technique.  Thanks in advance!

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InvisibleViolet
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Re: Invitro BRF or Grass Seed TEK – A powerful single-stage grow method for beginners and beyond [Re: Labmonkey43a]
    #21563343 - 04/18/15 11:49 AM (9 years, 1 month ago)

Here's your tip:  be patient!
You've done it right. (As far as we could tell). With a little gas exchange from a loosened lid, digestion and metabolism will speed up and before terribly long you'll see pins.

I'm curious as to how you could have such great success with mycelium but not see pins.  Many of us sometimes have to attempt avoiding pins at times and places we don't want them.

Regardless, this method should do nicely for you.

Did I see you mention casing? With grass seed, casing is not necessary. It doesn't look like you have casing layers there... So did you mean consolidation or something?
If so, don't sweat it. The time factor with this technique is simply reduced to waiting.  It's nearly impossible to fuck these up unless you're intrusive on them and do weird and unnecessary stuff.


--------------------
Intentionally or not, here in mushcult we are purveyors of love culture and enlightenment movement. Let's try to act like it!

PODS TEK - Growing Invitro with BRF/verm or Grass Seed containers
The simplest, quickest, safest tek!  For beginners, culturers, lazy people, stealth lovers, contam haters, and alternative seekers!
Violet's Teks and Posts

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InvisibleGrey
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Re: Invitro BRF or Grass Seed TEK – A powerful single-stage grow method for beginners and beyond [Re: Violet]
    #21563465 - 04/18/15 12:13 PM (9 years, 1 month ago)

I never saw this thread. I have some of these going though.



I have one made in an old Ragu jar. I'll shoot some Atl. #7 into it to see how those do.


--------------------


:takingnotes:  AMU Q&A  :takingnotes:


If you don't have a plan of your own, you'll become a part of somebody else's.

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OfflineLabmonkey43a
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Registered: 11/29/14
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Re: Invitro BRF or Grass Seed TEK – A powerful single-stage grow method for beginners and beyond [Re: Violet]
    #21563606 - 04/18/15 12:42 PM (9 years, 1 month ago)

Thanks Violet

I meant consolidation :blush:

I haven't seen pins on any cubes in any of my attempts.  I've seen crazy antenna looking mycelium that have 'stalks' 3/4" long and horizontal discs about 1/2" across, jars with ropey conduits that look like river creeping along a map,  super fuzzy and wispy, super dense and cakey...no pins in any of it.  (growing mycelium since December in at least 3 dozen jars)

My early attempts at a monotub picked up mold (noobed it up by peeking [it smelled so good though]).  I really like your tec here because it sounds super idiot proof.  It allows for super easy small-scale strain testing AND quarantines any contam in a localized area (which would be caused by idiocy with my gear) :laugh:

Thanks for the vote of confidence!  I'll make sure I post up results or frustrations.  I have enough various samples of different strains and stuff in flight that something should eventually work.  right?  right?  :laugh:

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Offlineblackout
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Re: Invitro Grass Seed TEK – A powerful single-step grow method for beginners and beyond [Re: Violet]
    #21563700 - 04/18/15 01:03 PM (9 years, 1 month ago)

Quote:

Violet said:
Added the PF-tek recipe and sterilization to the tek!

This tek works wonderfully for absolute beginners who don't even have a pressure cooker!



RGS could also be done without a PC, doing fractional sterilization (properly do not follow the teks you see about).

Rye grass seed is one grain which seems to be very forgiving moisture wise, in that the grain does not really explode. So you can add excess moisture and boil it off in a microwave over subsequent periods (10-12hr, not 24hr intervals). If you monitor the weight of your jars, grain and water you can calculate your moisture losses. If it gets too dry before your final heat treatment you can just open it and add more water in open air, as any newly introduced contams should be killed by the subsequent heat treatment.

By having lots of water at the start it will aid in heat transfer in both steaming and microwaving.

You could also try filling the jars with more. This is a commercial growkit from mushmush over 10 years ago. I got one but fucked it up. It was a great idea. There was a mini plastic vial/chamber of spores inside the lid. The lid was filtered, you simply pressed a button on the cap from the outside and the little chamber of spores broke open and released a few ml of spore solution. So no flaming needles or anything. The spores then grew on the RGS and you shook it and grew invitro shrooms.

These are not my pics.






http://www.shroomery.org/forums/showflat.php/Number/1816651/fpart/1/vc/1

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OfflineMushSicle
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Re: Invitro Grass Seed TEK – A powerful single-step grow method for beginners and beyond [Re: blackout]
    #21568063 - 04/19/15 01:23 PM (9 years, 1 month ago)

Violet, think this tek of yours would work for truffles as well, with slight mods? And what's the typical time from start to finish with this method?

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Offlineblackout
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Re: Invitro Grass Seed TEK – A powerful single-step grow method for beginners and beyond [Re: MushSicle]
    #21580929 - 04/22/15 04:39 PM (9 years, 28 days ago)

The mushmush kit was called an "instant mushrooms"

Here is a thread about it by Una from mushmush

http://www.shroomery.org/forums/showflat.php/Number/1568007/fpart/all/vc/1

I don't think they revealed the strain but people guessed it might be cambos, which were shown growing uncased on their site. Some strains are said to do better invitro than others. PF classic would be one. There were more photos in this thread

http://mycotopia.net/archives/discus/messages/5/29468.html?1061472216

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OfflineLabmonkey43a
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Re: Invitro Grass Seed TEK – A powerful single-step grow method for beginners and beyond [Re: blackout]
    #21600762 - 04/26/15 07:05 PM (9 years, 23 days ago)

Hi Everyone,

Thought I'd follow up to my question with a pic.


This is a wide mouth pint jar with Treasure Coast.  I had room in the "loud pot" (as the family calls it) and thought I'd try a small-scale version of the quart containers.  It has only 1/2 cup of seed (to keep the 'material:container' ratio)

This fruited first of all attempts, so I can assume the larger-scale versions will follow in a short period of time.  :grin:



Hooray!  Thanks for the confident thoughts


Someone asked earlier how long the process took:
This was colonized with WBS on 3/24, jar cracked on 4/13, today is 4/26.

I have others in this time range that aren't doing anything yet...it's just going to take patience.  I think keeping the room a little warmer will speed things along nicely.

Thanks for the great instructions and gentle guidance, Violet!

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OfflineDaveO
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Registered: 04/27/15
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Last seen: 8 years, 8 months
Re: Invitro Grass Seed TEK – A powerful single-step grow method for beginners and beyond [Re: Labmonkey43a]
    #21603744 - 04/27/15 12:12 PM (9 years, 23 days ago)

Sorry to be a nuisance as you will probably call me by the end of the post, but we all have to start somewhere
Well I think I'm missing something within this whole topic
How do you do this with BRF?
Do you make up your BRF jar, PC it, and then colonize your jar then once fully colonized you transfer to the quart jar? Or do you make your BRF mixture in the quart jars and sterilize in the quart jar ? Please someone answer this, I'm inexperienced, I'm sure anybody with any knowledge of this could help, thanks :smile:

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