Home | Community | Message Board

Cannabis Seeds - Original Sensible Seeds
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: North Spore Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies   Myyco.com Isolated Cubensis Liquid Culture For Sale   OlympusMyco.com Olympus Myco Sterilized Grain Bag for Spawn

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
OfflineTonyHxC
Knowledge Seeker
Male


Registered: 02/23/15
Posts: 123
Last seen: 1 year, 8 days
Made some flow charts for BRF, Agar and LC
    #21415342 - 03/16/15 10:44 AM (9 years, 2 months ago)

Hey guys, So I made these up more so to help my self but someone might find them useful. I will be adding more flow charts as I learn more (such as for WBS Jars etc)

I idea behind this flow chart is that you have already read over the appropriate teks. This is more of a guide line to follow so that you don't have to keep trying to find the appropriate paragraph in a document of whatever tek you are doing.

Feedback is very welcome :smile:






Edited by TonyHxC (03/16/15 10:47 AM)

Extras: Filter Print Post Top
InvisibleMunchauzen
 User Gallery

Registered: 06/22/11
Posts: 14,346
Trusted Cultivator
Re: Made some flow charts for BRF, Agar and LC [Re: TonyHxC]
    #21415476 - 03/16/15 11:24 AM (9 years, 2 months ago)

I wouldn't bother with fractional sterilization for agar. However, you can just steam it in a pot like pf-cakes.

looks like you have things figured out, though. and I like the way you did it visually with a mind-map. I've used them a lot before in my design work to help visualize your process.

Extras: Filter Print Post Top
OfflineTonyHxC
Knowledge Seeker
Male


Registered: 02/23/15
Posts: 123
Last seen: 1 year, 8 days
Re: Made some flow charts for BRF, Agar and LC [Re: Munchauzen]
    #21415483 - 03/16/15 11:26 AM (9 years, 2 months ago)

I am a software developer so I tend to make a lot of charts to figure things out haha. Thanks for your feedback. 

So you are saying that I can sterilize agar like like I would for PF-Cakes?  Should the water come up further on the jars then it does for PF Tek. Also how long? Would half hour be suffice.

Extras: Filter Print Post Top
InvisibleMunchauzen
 User Gallery

Registered: 06/22/11
Posts: 14,346
Trusted Cultivator
Re: Made some flow charts for BRF, Agar and LC [Re: TonyHxC]
    #21415497 - 03/16/15 11:31 AM (9 years, 2 months ago)

water should be below the jars. I elevate my base with levels of jar bands and then a sheet of aluminum foil.

I don't know of any solid facts to give you for steaming agar. I've done it personally for 2 hours and it worked. That was probably overkill. Maybe start with an hour or more to be on the safe side.

Extras: Filter Print Post Top
OfflineTonyHxC
Knowledge Seeker
Male


Registered: 02/23/15
Posts: 123
Last seen: 1 year, 8 days
Re: Made some flow charts for BRF, Agar and LC [Re: Munchauzen]
    #21415509 - 03/16/15 11:35 AM (9 years, 2 months ago)

Awesome, Thanks. I will give it and shot tonight and see what happens :smile:

Extras: Filter Print Post Top
OfflineTonyHxC
Knowledge Seeker
Male


Registered: 02/23/15
Posts: 123
Last seen: 1 year, 8 days
Re: Made some flow charts for BRF, Agar and LC [Re: TonyHxC]
    #21416074 - 03/16/15 01:45 PM (9 years, 2 months ago)

Also whipped this one up for PF-Tek.


Extras: Filter Print Post Top
InvisiblebodhisattaMDiscordReddit
Smurf real estate agent
 User Gallery
Folding@home Statistics
Registered: 04/30/13
Posts: 61,891
Loc: Milky way
Trusted Cultivator
Re: Made some flow charts for BRF, Agar and LC [Re: TonyHxC]
    #21416127 - 03/16/15 01:57 PM (9 years, 2 months ago)

when you sterilize even PF jars the steam is what does the sterilization. it's fine if the water comes up the side of the jars a bit but for agar cakes grain jars etc... the water in your steamer or PC shouldn't be more than half way up the side of anything.

Extras: Filter Print Post Top
OfflineTonyHxC
Knowledge Seeker
Male


Registered: 02/23/15
Posts: 123
Last seen: 1 year, 8 days
Re: Made some flow charts for BRF, Agar and LC [Re: bodhisatta]
    #21416200 - 03/16/15 02:14 PM (9 years, 2 months ago)

So what is the general opinion on Sterilizing Agar or LC in a large pot? I rarely see a pot given as an option in most of those tek's

Extras: Filter Print Post Top
InvisiblebodhisattaMDiscordReddit
Smurf real estate agent
 User Gallery
Folding@home Statistics
Registered: 04/30/13
Posts: 61,891
Loc: Milky way
Trusted Cultivator
Re: Made some flow charts for BRF, Agar and LC [Re: TonyHxC]
    #21416214 - 03/16/15 02:20 PM (9 years, 2 months ago)

most people never fucked around with not using a PC so why write it into the tek unless you had fist hand experience. you really should just pony up and buy a sterilizer or a PC. it takes less time, agar is easy to sterilize though 30m steam should be sufficient for a 500ml bottle.

Edited by Trusted cuItivator (03/16/15 02:21 PM)

Extras: Filter Print Post Top
Offlineblojo02184
Big Red
Male User Gallery


Registered: 05/15/13
Posts: 3,527
Loc: Maine
Last seen: 3 months, 4 days
Re: Made some flow charts for BRF, Agar and LC [Re: TonyHxC]
    #21416222 - 03/16/15 02:22 PM (9 years, 2 months ago)

Yeah... he's thinking of the making of the agar.
Heating it up in a pot before sterilization helps dissolve so of the solids left after mixing.

You do need to pressure cook agar bottles for 40 min at 15psi.

Extras: Filter Print Post Top
InvisiblebodhisattaMDiscordReddit
Smurf real estate agent
 User Gallery
Folding@home Statistics
Registered: 04/30/13
Posts: 61,891
Loc: Milky way
Trusted Cultivator
Re: Made some flow charts for BRF, Agar and LC [Re: blojo02184]
    #21416235 - 03/16/15 02:26 PM (9 years, 2 months ago)

20m at 15psi is the longest I have ever gone with agar. I usually do 15m and that's with 1 or 2 500ml bottles

Extras: Filter Print Post Top
OfflineTonyHxC
Knowledge Seeker
Male


Registered: 02/23/15
Posts: 123
Last seen: 1 year, 8 days
Re: Made some flow charts for BRF, Agar and LC [Re: bodhisatta]
    #21416287 - 03/16/15 02:40 PM (9 years, 2 months ago)

I plan on buying a PC soon, decent sized ones with gauges are expensive though, so I am just saving up my pennies.

So sterlizing Agar or LC using a large pot is ok but it just takes longer?

Extras: Filter Print Post Top
InvisiblebodhisattaMDiscordReddit
Smurf real estate agent
 User Gallery
Folding@home Statistics
Registered: 04/30/13
Posts: 61,891
Loc: Milky way
Trusted Cultivator
Re: Made some flow charts for BRF, Agar and LC [Re: TonyHxC]
    #21416291 - 03/16/15 02:41 PM (9 years, 2 months ago)

I would go at least 30m with steam. I have used steamed agar and even steamed LC before I think I did 45m or something. 30 should be enough. I always just use the PC for 15m

Extras: Filter Print Post Top
OfflineTonyHxC
Knowledge Seeker
Male


Registered: 02/23/15
Posts: 123
Last seen: 1 year, 8 days
Re: Made some flow charts for BRF, Agar and LC [Re: bodhisatta]
    #21416322 - 03/16/15 02:50 PM (9 years, 2 months ago)

Awesome, I fully intend on switching to PC once the funds come in. I am actually going for an interview this week for a new job :smile:

Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: North Spore Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies   Myyco.com Isolated Cubensis Liquid Culture For Sale   OlympusMyco.com Olympus Myco Sterilized Grain Bag for Spawn


Similar ThreadsPosterViewsRepliesLast post
* cheapest glass petri dishes, agar, dextrose, etc Fluxburn 7,496 8 02/14/05 07:57 AM
by Roadkill
* innoculating with old brf cakes flow 2,756 6 01/13/03 10:55 AM
by Anno
* test tubes agar fungophiliac 3,193 8 06/19/20 09:41 PM
by gorilliam
* If you ever get around to building a Flow Hood........... agar 1,270 1 12/30/04 01:25 AM
by 13eetleJuice
* Laminar flow question shambler 1,334 5 01/23/05 05:27 PM
by Anno
* Flow hood to dry shrooms? deafpanda 643 3 11/24/04 01:01 PM
by Ferment
* Buying large quanities of BRF & Vermiculite
( 1 2 all )
Fluxburn 4,234 24 02/01/05 06:10 PM
by mycofile
* Potato Dextrose Agar X Malt Extract Agar Alegalize 6,281 8 02/25/05 11:03 AM
by agar

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
943 topic views. 27 members, 141 guests and 44 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.073 seconds spending 0.005 seconds on 12 queries.