Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: PhytoExtractum Kratom Powder for Sale   OlympusMyco.com Olympus Myco Grow Bags   Unfolding Nature Unfolding Nature: Being in the Implicate Order   Myyco.com Isolated Cubensis Liquid Culture For Sale   MagicBag.co All-In-One Bags That Don't Suck   North Spore Injection Grain Bag   Original Sensible Seeds Bulk Cannabis Seeds   Left Coast Kratom Buy Kratom Capsules   Mushroom-Hut Liquid Cultures

Jump to first unread post Pages: 1 | 2 | Next >  [ show all ]
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
OfflineFeedYourMind
indiGlo
 User Gallery


Registered: 03/17/14
Posts: 1,155
Loc: That One Place
Last seen: 1 day, 19 hours
Learning to be patient...
    #21412953 - 03/15/15 07:34 PM (9 years, 2 months ago)

So I have some B+  and Argentina in quart jars. 5 for each. This is not my first rodeo but I have never experienced growth this slow. In fact, I have 4 out of 10 jars with no growth at all. And yes, this was MS syringe from a trusted sponsor I've utilized many times before with a high degree of success. But still - no growth. Not even contams. Because of the altitiude I'm required to pc longer which I did, 2 hrs.

I had soaked my wbs 24 hours prior to pc, and then simmered until the grains were plump and tasteless. Laid out after draining and then loaded into jars. Tyvek filter with micropore tape over innoc hole, (4) 1/16 holes for GE. This has been my go to method with great success as I also innoc in a SAB per the tek. It's never failed me... Until now.

What should I do from here? Continue to be patient? I'm 2 weeks in from inoculation and this is what I have to show on my fastest jar:



And a quarter turn... This picture represents about how far my other jars have grown, not much.



And then my jars with nothing:



I'm going to make a new order with a new trusted vendor I've seen receive high praise here. I already have plenty of jars and wbs to innoc. I know I need to do some agar and I have some brf substitute going (6 jars per) but no sign of life yet in any of them.

Has anyone run into this problem before?
Either way, I'm learning to let nature do its thing and not be the pushy prize winner I am.


--------------------

Extras: Filter Print Post Top
Invisibleblindingleaf
blue collar underworld
 User Gallery

Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
Trusted Cultivator
Re: Learning to be patient... [Re: FeedYourMind]
    #21413099 - 03/15/15 08:06 PM (9 years, 2 months ago)

did u shake the jar after u inoculated with syringe?


--------------------
A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

The whole is greater than the sum of its parts

Extras: Filter Print Post Top
OfflineFeedYourMind
indiGlo
 User Gallery


Registered: 03/17/14
Posts: 1,155
Loc: That One Place
Last seen: 1 day, 19 hours
Re: Learning to be patient... [Re: blindingleaf]
    #21413149 - 03/15/15 08:16 PM (9 years, 2 months ago)

Quote:

blindingleaf said:
did u shake the jar after u inoculated with syringe?




I did not.
It's perplexing, I know.

What do you think the deal is here?


--------------------

Edited by FeedYourMind (03/15/15 08:18 PM)

Extras: Filter Print Post Top
Invisibleblindingleaf
blue collar underworld
 User Gallery

Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
Trusted Cultivator
Re: Learning to be patient... [Re: FeedYourMind]
    #21413168 - 03/15/15 08:19 PM (9 years, 2 months ago)

its been a really long time since i injected grains with a syringe, but, i would wait it out.  they look clean otherwise, like u mentioned.
i would shake when it doubles it current size and see what happens.

try using pints as masters next time.
whether its a spore syringe, agar wedge, LI, or LC, its faster to colonize, so u can g2g earlier, and spawn quicker.


--------------------
A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

The whole is greater than the sum of its parts

Extras: Filter Print Post Top
OfflineFeedYourMind
indiGlo
 User Gallery


Registered: 03/17/14
Posts: 1,155
Loc: That One Place
Last seen: 1 day, 19 hours
Re: Learning to be patient... [Re: blindingleaf]
    #21413208 - 03/15/15 08:29 PM (9 years, 2 months ago)

Quote:

blindingleaf said:
its been a really long time since i injected grains with a syringe, but, i would wait it out.  they look clean otherwise, like u mentioned.
i would shake when it doubles it current size and see what happens.

try using pints as masters next time.
whether its a spore syringe, agar wedge, LI, or LC, its faster to colonize, so u can g2g earlier, and spawn quicker.




Yeah, I was starting anew and had to go that route unfortunately. I will def do the pint idea! I have some here so I'll make it a point to do a few of them as well as I will be gathering supplies tomorrow to do a more reliable and proven agar tek.

And yes! I'll be sure to shake. I figured it would be too small to do now, so you answered my next question. Thank you!

One last question though - what do you propose I do with the jars without growth?


--------------------

Extras: Filter Print Post Top
Invisibleblindingleaf
blue collar underworld
 User Gallery

Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
Trusted Cultivator
Re: Learning to be patient... [Re: FeedYourMind]
    #21413248 - 03/15/15 08:41 PM (9 years, 2 months ago)

the jars w/o growth, not sure.
i've seen people say to resterilize if there is no growth, but also no contam.
i never had that issue, so maybe wait for someone else to post.
i usually am too paranoid to do that, but in ur case, it might be reasonable, since there isn't anything growing anyway, good or bad.

i know u mentioned it, buy i would try really hard to get some agar going, be it no pour plates, or standard pour plates.
it will help eliminate a lot of variables, with both MS or clones.

happy cultivating brother!


--------------------
A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

The whole is greater than the sum of its parts

Extras: Filter Print Post Top
Offlinemushpunx
Fungus Punk
 User Gallery


Registered: 04/20/14
Posts: 13,394
Last seen: 4 days, 17 hours
Trusted Cultivator
Re: Learning to be patient... [Re: blindingleaf]
    #21413292 - 03/15/15 08:57 PM (9 years, 2 months ago)

Quote:

blindingleaf said:
its been a really long time since i injected grains with a syringe, but, i would wait it out.  they look clean otherwise, like u mentioned.
i would shake when it doubles it current size and see what happens.

try using pints as masters next time.
whether its a spore syringe, agar wedge, LI, or LC, its faster to colonize, so u can g2g earlier, and spawn quicker.





Yea I actually didnt consider that, using a pint to inoculate a spawn bag Ive been just pouring 1 quart into two bags. But I have a hundred pint jars upstairs from when I had a smaller PC.... awesome! Right on!


--------------------

Amateur Mycologists United
AMU Q&A

Extras: Filter Print Post Top
OfflineFeedYourMind
indiGlo
 User Gallery


Registered: 03/17/14
Posts: 1,155
Loc: That One Place
Last seen: 1 day, 19 hours
Re: Learning to be patient... [Re: blindingleaf]
    #21413873 - 03/15/15 11:14 PM (9 years, 2 months ago)

Quote:

blindingleaf said:
the jars w/o growth, not sure.
i've seen people say to resterilize if there is no growth, but also no contam.
i never had that issue, so maybe wait for someone else to post.
i usually am too paranoid to do that, but in ur case, it might be reasonable, since there isn't anything growing anyway, good or bad.

i know u mentioned it, buy i would try really hard to get some agar going, be it no pour plates, or standard pour plates.
it will help eliminate a lot of variables, with both MS or clones.

happy cultivating brother!




You too my friend! +5 for the help.
4 jars are resterilizing in pc for two hours and money deposited; about to order some syringes from a different vendor as well as plates and all my agar material.

Let's do this motha truckas. :bananahorsey:


--------------------

Edited by FeedYourMind (03/15/15 11:17 PM)

Extras: Filter Print Post Top
InvisibleTheEaglesGift
The Nagual


Registered: 04/10/11
Posts: 10,554
Loc: Ixtlan, Mexico
Trusted Cultivator
Re: Learning to be patient... [Re: FeedYourMind]
    #21414401 - 03/16/15 01:30 AM (9 years, 2 months ago)

Let them be and see how they turn out.

I'd order some new syringes and get a new project going ASAP. It helps to have multiple projects going on at once so that when one doesn't go well, the others will keep you cheered up and engaged. I hate down time and disappointments.

Learn agar. :thumbup:

I really wish someone had drilled into me to start agar when I was starting out, would've saved me a lot of time, wasted materials, and energy.

Extras: Filter Print Post Top
OfflineBoland
Male User Gallery


Registered: 01/12/15
Posts: 16
Loc: United Kingdom
Last seen: 9 years, 1 month
Re: Learning to be patient... [Re: TheEaglesGift]
    #21414604 - 03/16/15 05:28 AM (9 years, 2 months ago)

I had a similar situation waiting an age for growth
(I had one jar which was growing much faster also)

Turned out the temperature in my house was to low, winter time in England with a dodgy boiler is pretty cold

I moved them to a mates house and they have started growing a lot quicker
Still slow compared to previous grows, but all have growth and a after a month a couple are around 50/60%

I moved them after having a month of no growth on a couple and very little growth on the others

Might or might not be the issue

P.S. if temperature is cold the top of a fridge is a pretty decent place to keep them


--------------------
I want to die peacefully in my sleep like my grandfather, not screaming in terror like his passengers :grin:

Extras: Filter Print Post Top
OfflineAchillita
Back to the basics
Male


Registered: 05/26/14
Posts: 4,565
Last seen: 3 years, 3 months
Re: Learning to be patient... [Re: Boland]
    #21414828 - 03/16/15 07:48 AM (9 years, 2 months ago)

Some spore syringes don't come out right, even from trusted vendors. The first few times I started growing, I got 3 syringes and none of them worked. I think it might have been old spores or something weird. I eventually got a random one and it worked out and have been slowly growing my spore bank


--------------------

Extras: Filter Print Post Top
OfflineFeedYourMind
indiGlo
 User Gallery


Registered: 03/17/14
Posts: 1,155
Loc: That One Place
Last seen: 1 day, 19 hours
Re: Learning to be patient... [Re: Achillita]
    #21415912 - 03/16/15 01:11 PM (9 years, 2 months ago)

I've learned agar and used it successfully.
However, because of transportation and my access to limited supplies, I wasn't able to use MLE, agar-agar, and Petri dishes... Those have just been ordered.

I'm happy to report that being patient has its perks. I woke up this morning and all 12 of my brf substitute jars have some nice growth on them. Ready to innoc with wedges and make some isolates! :grin:

Quote:

Boland said:
I had a similar situation waiting an age for growth
(I had one jar which was growing much faster also)

Turned out the temperature in my house was to low, winter time in England with a dodgy boiler is pretty cold

I moved them to a mates house and they have started growing a lot quicker
Still slow compared to previous grows, but all have growth and a after a month a couple are around 50/60%

I moved them after having a month of no growth on a couple and very little growth on the others

Might or might not be the issue

P.S. if temperature is cold the top of a fridge is a pretty decent place to keep them




I believe this was part of the issue because I stuck a small, efficient space heater in a half opened closet and allowed the temps to get to 78/79F then figured out how to maintain it. My jars are loving it and growing a bit faster it seems. Thanks for the input!


Quote:

Achillita said:
Some spore syringes don't come out right, even from trusted vendors. The first few times I started growing, I got 3 syringes and none of them worked. I think it might have been old spores or something weird. I eventually got a random one and it worked out and have been slowly growing my spore bank




So much truth to this. I know my syringe was made fresh as I was in contact with the vendor - and he's not one to lie. I think it could have been a combination of all of the above as well as a user error on my part.

Let me ask you kind people this:
Do you flame and wipe with alchohol? I have been, but I hear and feel this gaseous sound once I inoculate into the jars. I wonder if I'm not letting it cool down enough and perhaps damaging the spores? Just a theory.


--------------------

Extras: Filter Print Post Top
Offlinemushpunx
Fungus Punk
 User Gallery


Registered: 04/20/14
Posts: 13,394
Last seen: 4 days, 17 hours
Trusted Cultivator
Re: Learning to be patient... [Re: FeedYourMind]
    #21416409 - 03/16/15 03:08 PM (9 years, 2 months ago)

Youre talking about a needle? You can wipe with alchohol (I dont) as long as you do it *before* you flame sterilize.

Wiping afterwards will make it unsterile


--------------------

Amateur Mycologists United
AMU Q&A

Extras: Filter Print Post Top
InvisibleTheEaglesGift
The Nagual


Registered: 04/10/11
Posts: 10,554
Loc: Ixtlan, Mexico
Trusted Cultivator
Re: Learning to be patient... [Re: mushpunx]
    #21416555 - 03/16/15 03:45 PM (9 years, 2 months ago)

Quote:

mushpunx said:
Youre talking about a needle? You can wipe with alchohol (I dont) as long as you do it *before* you flame sterilize.

Wiping afterwards will make it unsterile



This.

Extras: Filter Print Post Top
OfflineFeedYourMind
indiGlo
 User Gallery


Registered: 03/17/14
Posts: 1,155
Loc: That One Place
Last seen: 1 day, 19 hours
Re: Learning to be patient... [Re: mushpunx]
    #21418772 - 03/17/15 12:18 AM (9 years, 2 months ago)

Quote:

mushpunx said:
Youre talking about a needle? You can wipe with alchohol (I dont) as long as you do it *before* you flame sterilize.

Wiping afterwards will make it unsterile




I've been wiping with alcohol prior to innoc and right after flaming - thinking I was adding a precautionary measure. Looks like I really was a bit backwards. Thank you!


--------------------

Extras: Filter Print Post Top
InvisibleTheEaglesGift
The Nagual


Registered: 04/10/11
Posts: 10,554
Loc: Ixtlan, Mexico
Trusted Cultivator
Re: Learning to be patient... [Re: FeedYourMind]
    #21418779 - 03/17/15 12:20 AM (9 years, 2 months ago)

Alcohol=sanitary
Flame=sterile

Extras: Filter Print Post Top
OfflineFeedYourMind
indiGlo
 User Gallery


Registered: 03/17/14
Posts: 1,155
Loc: That One Place
Last seen: 1 day, 19 hours
Re: Learning to be patient... [Re: TheEaglesGift]
    #21418790 - 03/17/15 12:26 AM (9 years, 2 months ago)

Quote:

TheEaglesGift said:
Alcohol=sanitary
Flame=sterile




Dually noted. Thank you!


--------------------

Extras: Filter Print Post Top
OfflineFeedYourMind
indiGlo
 User Gallery


Registered: 03/17/14
Posts: 1,155
Loc: That One Place
Last seen: 1 day, 19 hours
Re: Learning to be patient... [Re: FeedYourMind]
    #21424294 - 03/17/15 09:02 PM (9 years, 2 months ago)

And for those who were wondering..

I figured out what I did wrong - did not cook the grains long enough. I loaded some half pint jars with WBS to act as my master jars and after comparing the jars, there is a noticeable difference.

These most recent jars grains are soft and plump so I transferred some agar wedges into it and it's taking off. So if you have no contams and hardly any growth, this might be your issue to.



See the difference?


Cook ze grains!


--------------------

Edited by FeedYourMind (03/17/15 09:07 PM)

Extras: Filter Print Post Top
InvisibleAlgo
Backstrap Fever


Registered: 12/15/14
Posts: 3,857
Re: Learning to be patient... [Re: FeedYourMind]
    #21424305 - 03/17/15 09:04 PM (9 years, 2 months ago)

To be honest i bought some spores that were bunk one time from a sponser on this site. But all the others i got from them were awesome.


--------------------

Extras: Filter Print Post Top
OfflineFeedYourMind
indiGlo
 User Gallery


Registered: 03/17/14
Posts: 1,155
Loc: That One Place
Last seen: 1 day, 19 hours
Re: Learning to be patient... [Re: Algo]
    #21424342 - 03/17/15 09:10 PM (9 years, 2 months ago)

It happens. I think it's happened twice to me now, but I believe my methods are suspect as well
Time to clean up my act! :grin:


--------------------

Extras: Filter Print Post Top
Jump to top Pages: 1 | 2 | Next >  [ show all ]

Shop: PhytoExtractum Kratom Powder for Sale   OlympusMyco.com Olympus Myco Grow Bags   Unfolding Nature Unfolding Nature: Being in the Implicate Order   Myyco.com Isolated Cubensis Liquid Culture For Sale   MagicBag.co All-In-One Bags That Don't Suck   North Spore Injection Grain Bag   Original Sensible Seeds Bulk Cannabis Seeds   Left Coast Kratom Buy Kratom Capsules   Mushroom-Hut Liquid Cultures


Similar ThreadsPosterViewsRepliesLast post
* Innoculating Unsterile Jars JohnP 664 2 02/28/09 08:46 PM
by libertaire
* Growing in unsterile environments??? filthysock 1,599 6 03/03/04 08:31 AM
by peruvian spark
* My unsterile retarded LC worked, beginner's luck :o iamsmooth 1,554 14 09/03/08 07:14 PM
by libertaire
* Resterilizing? Booming 733 8 12/15/06 10:11 PM
by Blutjager
* resterilizing syringes shrub 756 8 01/28/10 04:44 AM
by blood4blood
* Nothing growing on WBS jars - Resterilize or Just reinject? pftek 1,082 7 04/05/09 07:26 PM
by NineInchNails
* resterilizing contaminated grain mistermisfit 1,180 7 10/24/10 06:27 PM
by k00laid
* Innoculated resterilized contaminated jar... Swami 3,315 8 04/10/18 11:44 AM
by Shroomarican

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
1,505 topic views. 19 members, 112 guests and 38 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.027 seconds spending 0.006 seconds on 13 queries.