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OfflineCallampin
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Registered: 08/14/03
Posts: 520
Last seen: 6 months, 18 days
Syringes dying cause of low temp?
    #2141195 - 11/27/03 09:43 PM (20 years, 4 months ago)

4 days ago i innoced some jars and 5 minutes ago i whent to check on them , and nuthing , these syrimnges were creeper and pesh and they were made and used inmediatly and the rest wich were 5 more syringes were kept in the fridge for less than 3 weeks , so i dont know whats up with these shits , it looks like dead spores or some thing cause the rye doesnt have a single trace of myc

is it posible that the low temp killed them??

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Invisiblesoochi
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Registered: 08/13/02
Posts: 2,420
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Re: Syringes dying cause of low temp? [Re: Callampin]
    #2141204 - 11/27/03 09:48 PM (20 years, 4 months ago)

low temps makes spores go dormant, 4 days is NOTHING!!. Do me a favor, leave your jars in incubation (dark and warm) for a week. Don't think about them, prod, poke, touch, smell, etc (I know it's hard :smirk:) "The less you mess, the more success"  :grin:


Some of my jars have been known to take up to two weeks before you see any signs of colonization (especially at room temp)




--------------------
Wee, sleeket, cowran, tim'rous beastie,
O, what panic's in thy breastie!
Thou need na start awa sae hasty,
Wi' bickering brattle!

Edited by soochi (11/27/03 09:50 PM)

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Offlinefidget
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Registered: 03/18/02
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Loc: Oregon
Last seen: 1 year, 7 months
Re: Syringes dying cause of low temp? [Re: Callampin]
    #2141210 - 11/27/03 09:49 PM (20 years, 4 months ago)

Its only been 4 days. If you see nothing at 8-10 days then you can start to worry. And did you let the spores reydrate for 24 hours before you used the syringe?


--------------------
- fidget

"With ordinary consciousness you can't even begin to know what's happening."

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OfflineCallampin
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Registered: 08/14/03
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Last seen: 6 months, 18 days
Re: Syringes dying cause of low temp? [Re: soochi]
    #2141212 - 11/27/03 09:50 PM (20 years, 4 months ago)

Quote:

"The less you mess, the more success" 




:grin: :grin: :grin: :grin: :grin: :grin: :grin: :grin: 

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OfflineCallampin
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Registered: 08/14/03
Posts: 520
Last seen: 6 months, 18 days
Re: Syringes dying cause of low temp? [Re: Callampin]
    #2141215 - 11/27/03 09:51 PM (20 years, 4 months ago)

whats that about "rehydrating"????

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Invisiblesoochi
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Re: Syringes dying cause of low temp? [Re: Callampin]
    #2141248 - 11/27/03 10:03 PM (20 years, 4 months ago)

Did you make the syringes yourself? if so, how old were the prints you made them from? older prints need a little rehydration to insure good results. If they were purchased then you have nothing to worry about.


--------------------
Wee, sleeket, cowran, tim'rous beastie,
O, what panic's in thy breastie!
Thou need na start awa sae hasty,
Wi' bickering brattle!

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OfflineShdwstr
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Registered: 02/17/01
Posts: 2,156
Loc: Ontario, Canada
Last seen: 10 years, 3 months
Re: Syringes dying cause of low temp? [Re: Callampin]
    #2141296 - 11/27/03 10:27 PM (20 years, 4 months ago)

When you make a syringe from a print, you need to let it sit in the syringe for at least 24 hrs. If it is an old print, a few days would'nt hurt.
I've rehydrated spores 10 years old with success by waiting a week before using the syringe.

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OfflineCallampin
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Registered: 08/14/03
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Last seen: 6 months, 18 days
Re: Syringes dying cause of low temp? [Re: Shdwstr]
    #2141307 - 11/27/03 10:30 PM (20 years, 4 months ago)

the syringes were sitting in the fridge for 3 weeks ,and the spores were from fsre

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OfflineShdwstr
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Male

Registered: 02/17/01
Posts: 2,156
Loc: Ontario, Canada
Last seen: 10 years, 3 months
Re: Syringes dying cause of low temp? [Re: Callampin]
    #2141360 - 11/27/03 10:57 PM (20 years, 4 months ago)

I've known frozen solid syringes to be fine after thawing.
Just be patient and see what happens over the next week.

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OfflineCallampin
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Registered: 08/14/03
Posts: 520
Last seen: 6 months, 18 days
Re: Syringes dying cause of low temp? [Re: Shdwstr]
    #2141379 - 11/27/03 11:14 PM (20 years, 4 months ago)

i am iam ...... :nut: :crazy2: :nut: :crazy2: :nut: :crazy2: 

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Anonymous

Re: Syringes dying cause of low temp? [Re: Callampin]
    #2142118 - 11/28/03 12:17 PM (20 years, 4 months ago)

fuck that,my foaf has a pointy needle thing sitting in the fridge for a month now and after going to all the trouble of innocying his jars thought "why use stale shit".putting in the fridge is for stretching.

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