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OfflineMultimono
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Registered: 06/12/14
Posts: 161
Last seen: 7 years, 10 months
Trich is getting the best of me :,( help
    #21401990 - 03/13/15 12:10 PM (9 years, 2 months ago)

Anyone that has a knowledgable input, please help. Afoaf has gotten trich on all past monos except for two rounds. It has always been before the first flush. Afoaf hear if it was the spores in the air then it would happen after the first flush most likely. Afoaf have had a couple contaminated wbs jars with trich each time, this time afoaf flame sterilized between each jar rather than every couple. Afoaf hasn't had any this time. The tubs have adoquiet fae also have a very nice hepa filter. Do you guys believe the problem originates from the wbs inoculation? Or the spore count in the air? Since afoaf have had no contam in the jars this time, do you belive the tubs will most likely come out contam free or after the first flush at least. When afloat has had no contams in jars the tubs have been perfect, but is worried about the spore count in the air. I know this post is all over the place in wording but I have seemed to have a very early 4/20.  Also afoaf do have carpet but Heshe lays a freshly cleaned with bleach blanket down before fruiting monos. Another important bit of information is when the tubs get trich Afoaf always has 1/2-3/4 of the tubs contam free each time.

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Offlinemushpunx
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Registered: 04/20/14
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Re: Trich is getting the best of me :,( help [Re: Multimono]
    #21402072 - 03/13/15 12:38 PM (9 years, 2 months ago)

Generally if its trich before the first flush its in your spawn.

So first off what are you innoculating with, spore syringe? Do you work in a still air box?

I dont know what you mean by the HEPA filter, you run one of those air purifying HEPAs like you would buy at wallmart in the room?

Walk us through your sterile procedure and process for making spawn.


Trich can hide very well in spawn, you often wont be able to tell until you put your sub into fruiting. This is why its essential to start out with clean innoculant.

If you dont already, you should learn to clean your culture on agar first and inoculate your jars with a wedge.

Friendly sugguestion.. spaces when you type haha. And cut out the AFOaF thing it wont protect you legally. Trich happens to the best of us so dont get discouraged!


Edited by mushpunx (03/13/15 12:50 PM)

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OfflineMultimono
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Registered: 06/12/14
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Re: Trich is getting the best of me :,( help [Re: mushpunx]
    #21402124 - 03/13/15 12:52 PM (9 years, 2 months ago)

Thanks for responding! I inoculate in open air but wipe the whole room down with bleach and alchahol. I inoculate with a ms syringe from a sponsor. I check it in agar dishes pre made. Then use the syringe to inoculate wbs transfer to tubs with Damian's 5050. Then fruit. Trick always happens before the first flush. One thing I recently changed is I now flame sterilize between each jar. This round has shown no contam in any jars. Before I would get a couple trich jars so that's a good sign.

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InvisibleT-Funkadelic
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Registered: 05/14/13
Posts: 11,393
Loc: 2535 W Fairmont Ave MD 21223 Flag
Re: Trich is getting the best of me :,( help [Re: Multimono]
    #21402141 - 03/13/15 12:56 PM (9 years, 2 months ago)

Always flame sterilize between jars. That could have been your problem.


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OfflineStromriderM
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Registered: 06/02/13
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Re: Trich is getting the best of me :,( help [Re: T-Funkadelic]
    #21402149 - 03/13/15 01:00 PM (9 years, 2 months ago)

If you know how to use agar then why in the world are you inoculating with a spore syringe. Why not use an agar wedge?

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OfflineMultimono
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Re: Trich is getting the best of me :,( help [Re: Stromrider]
    #21402166 - 03/13/15 01:05 PM (9 years, 2 months ago)

No flow hood or sab :frown: , I'm using my newly made of from agar next time. I just wonder if the flame sterilization was why I was getting trich or the spore count in the air.

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Offlinemushpunx
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Registered: 04/20/14
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Re: Trich is getting the best of me :,( help [Re: Multimono]
    #21402171 - 03/13/15 01:07 PM (9 years, 2 months ago)

Quote:

Multimono said:
Thanks for responding! I inoculate in open air but wipe the whole room down with bleach and alchahol. I inoculate with a ms syringe from a sponsor. I check it in agar dishes pre made. Then use the syringe to inoculate wbs transfer to tubs with Damian's 5050. Then fruit. Trick always happens before the first flush. One thing I recently changed is I now flame sterilize between each jar. This round has shown no contam in any jars. Before I would get a couple trich jars so that's a good sign.





Ahhh well your first problem is open air inoculation. Its never a good idea. I sugguest you build a still air box and learn how to use it, by keeping the air as still as possible and through controlled, descisive hand motions you can try to prevent contams from landing in your jar.

Second, its great that your using agar but you need to utilize it. Just checking to see if your syringe germinates wont help you.

No syringe is 100% clean and usually when I see germination from a syringe on agar the first plate is loaded with contams. I transfer a peice of healthy myc to a clean plate several times before I am sure I now have a clean culture, and then inoculate my master jar with a wedge of agar... and G2G after that.


Since you dont G2G , and you inoculate your whole round of spawn with a syringe thats the only real chance it has to get in most likely.


I think its your syringe.


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Offlinemushpunx
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Re: Trich is getting the best of me :,( help [Re: Multimono]
    #21402182 - 03/13/15 01:09 PM (9 years, 2 months ago)

Quote:

Multimono said:
No flow hood or sab :frown: , I'm using my newly made of from agar next time. I just wonder if the flame sterilization was why I was getting trich or the spore count in the air.





SAB cost ten bux and ten minutes to make, build it, love it!


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OfflineStromriderM
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Re: Trich is getting the best of me :,( help [Re: mushpunx]
    #21402188 - 03/13/15 01:11 PM (9 years, 2 months ago)

Build a sab
Learn proper sterile technique
Inoculate a grain master with a clean agar wedge
G2G from there


Should solve your problems


Trich is our Achilles heel. We've all fought it at some point. Hang in there

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OfflineMultimono
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Registered: 06/12/14
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Last seen: 7 years, 10 months
Re: Trich is getting the best of me :,( help [Re: Stromrider]
    #21402211 - 03/13/15 01:15 PM (9 years, 2 months ago)

i will have to look into getting materials for one (: . As for the trick before the first flush, it's most likely my spawn? Because now that I've flame sterilized between each jar instead of every three I havent had any viable contams in any of the jars.

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OfflineStraya
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Registered: 03/04/12
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Re: Trich is getting the best of me :,( help [Re: Stromrider]
    #21402221 - 03/13/15 01:18 PM (9 years, 2 months ago)

Buy a plastic storage container 75L

Burn two holes into it with a tin can (coffee, fruit or milo can) that has a diameter of 100mm or a little bit bigger.

Wipe down with isopropyl alcohol (70%) and you have a sterile work environment.

Still Air Box construction

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Offlinemushpunx
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Re: Trich is getting the best of me :,( help [Re: Multimono]
    #21402256 - 03/13/15 01:27 PM (9 years, 2 months ago)

Yea probably the spawn.

Flame sterilizing should DEF help though, and SAB will help too. Make best friends with agar

Like I said its good you dont se any signs of contam and best of luck, it doesnt mean youre in the clear though. Took a bit of convincing for me because my spawn looked and smelled tip top but tubs went green haha

You can help yourself by washing your tubs out with dish soap amd water, wearing gloves when you spawn keeping a clean area etc


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OfflineMultimono
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Re: Trich is getting the best of me :,( help [Re: mushpunx]
    #21402352 - 03/13/15 01:53 PM (9 years, 2 months ago)

Tubs are always washed with bleach and water then woped down with 70% ISO before placing plastic bag down. Do you use trash bag inside monos? Or would taping them be better?

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OfflineStraya
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Registered: 03/04/12
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Loc: Flag
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Re: Trich is getting the best of me :,( help [Re: Multimono]
    #21402354 - 03/13/15 01:54 PM (9 years, 2 months ago)

Almost pointless if you don't use a SAB

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OfflineMultimono
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Registered: 06/12/14
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Re: Trich is getting the best of me :,( help [Re: Straya]
    #21402362 - 03/13/15 01:56 PM (9 years, 2 months ago)

I'm talking about when I put the coir and spawn in

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OfflineKizzle
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Re: Trich is getting the best of me :,( help [Re: Multimono]
    #21402462 - 03/13/15 02:27 PM (9 years, 2 months ago)

Is your spawn completely colonizing? There aren't any uncolonized grains in them?


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OfflineHeisenburg
HOPE U LIKE WHAT U SEE!!!!
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Registered: 02/24/15
Posts: 113
Last seen: 9 years, 1 month
Re: Trich is getting the best of me :,( help [Re: Kizzle]
    #21402484 - 03/13/15 02:34 PM (9 years, 2 months ago)

Check out the SAB in my signature. I just put it together. Maybe 20 or 25 bucks.


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OfflineMultimono
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Registered: 06/12/14
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Last seen: 7 years, 10 months
Re: Trich is getting the best of me :,( help [Re: Heisenburg]
    #21402550 - 03/13/15 02:52 PM (9 years, 2 months ago)

I like that sab. And yes completely colonized now at least. I smoke quite a bit so generally I can't remember what all the jars looked like from the past.

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Offlinemushpunx
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Re: Trich is getting the best of me :,( help [Re: Multimono]
    #21402964 - 03/13/15 04:27 PM (9 years, 2 months ago)

Yea Kizzle is right even a few uncolonized grains can ruin your day


What kind of lids do you use on your grain jars?


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OfflineMultimono
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Registered: 06/12/14
Posts: 161
Last seen: 7 years, 10 months
Re: Trich is getting the best of me :,( help [Re: mushpunx]
    #21404074 - 03/13/15 09:19 PM (9 years, 2 months ago)

Tyvek and polyfil

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