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OfflinehamloafM
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Avoid The Noid With LC's
    #21401237 - 03/13/15 07:55 AM (9 years, 10 months ago)

Mycelium colonizing a liquid media.
Sometime chunks of live culture float to the top.
Once to the top, myce begins forming a hardened mat of mycelium.
This mat grows 2 dimensionally, right over the top of the medium. 



If left unchecked.
The myce is allowed to fully colonize the top of the liquid media.
Resulting in live culture underneath the mat to be killed due to lack of oxygen. 
Hard and matted liquid mycelium can often be difficult to aspire into syringes too.

Don't let this happen to you. 
Avoid the Noid.

Solution!:

Stir or shake your LC's.



Stirring or shaking will chop the mat up.
The mat will become liquid logged and sink into the media.



Noid defeated.
Repeat as needed.
Thank you.
Good luck!


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Offlineliquidclutter
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Re: Avoid The Noid With LC's [Re: hamloaf]
    #21402845 - 03/13/15 04:00 PM (9 years, 10 months ago)

thanks for sharing ham.

here are what some contaminated LC's will look.
Even started to make jelly chunks.



edit: may picture taking skills have improved from this. hell, i did not even wash the jar first :facepalm:


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Edited by liquidclutter (03/13/15 04:01 PM)

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OfflinehamloafM
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Re: Avoid The Noid With LC's [Re: liquidclutter]
    #21402873 - 03/13/15 04:07 PM (9 years, 10 months ago)

Look at you, you old devil-dog.  LET'S TAKE THIS FUCKIN' HILL!!!!  :lol:


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OfflineMushroom_J
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Re: Avoid The Noid With LC's [Re: hamloaf]
    #21402950 - 03/13/15 04:24 PM (9 years, 10 months ago)

how do you do your lids?


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OfflinehamloafM
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Re: Avoid The Noid With LC's [Re: Mushroom_J]
    #21403266 - 03/13/15 05:43 PM (9 years, 10 months ago)

PP5, plastic closures.
Whatman syringe filters for GE.
Soon to be modified further with large injection port.
Large injection port modification for LC gun access.

No gasket.
Where the lid meets the glass is triple wrapped in parafilm.





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OfflineYaMoonSun
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Re: Avoid The Noid With LC's [Re: hamloaf]
    #21403286 - 03/13/15 05:47 PM (9 years, 10 months ago)

What if you keep shaking it, and it constantly rises back to the top?

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OfflinehamloafM
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Re: Avoid The Noid With LC's [Re: YaMoonSun]
    #21403351 - 03/13/15 06:06 PM (9 years, 10 months ago)

Aspire what you can of it right away.
The matted myce'll be broke up enough to be aspired.
Some of the matted myce will be hard to aspire still.
Use 12 gauge, blunt-tipped dispensing needles.





In that situation, there's really no replacement for magnetic stirring devices.
Magnetic stirring devices create a cyclone within the media that'll keep those pesky Noids under media.
Until they are media logged enough to sink without the aid of being stirred magnetically.


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Offlineinvitro

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Re: Avoid The Noid With LC's [Re: hamloaf]
    #21403374 - 03/13/15 06:15 PM (9 years, 10 months ago)

:takingnotes:

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Offlinetripdawg420
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Re: Avoid The Noid With LC's [Re: invitro]
    #21403415 - 03/13/15 06:30 PM (9 years, 10 months ago)

:thumbup:


--------------------
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How U Survive This Life Everyday Resourcefully
epic GT mono tub
http://www.shroomery.org/forums/showflat.php/Number/17277772

wbs tek
http://www.shroomery.org/forums/showflat.php/Number/11525679
coir tek
http://www.shroomery.org/forums/showflat.php/Number/11917410
results :thumbup:

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Offlineliquidclutter
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Re: Avoid The Noid With LC's [Re: tripdawg420]
    #21403486 - 03/13/15 06:56 PM (9 years, 10 months ago)

:bow2:


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Invisibleeatyualive
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Re: Avoid The Noid With LC's [Re: liquidclutter]
    #21404033 - 03/13/15 09:09 PM (9 years, 10 months ago)

im gonna give your grain liquid cultures a go pretty soon. im making up some plates in the next week or so to start some things off. first i have to soak some grain. its been close to 15 years since ive done that.:lol:

almost time to dust off the old stir plate and stir bars. i have those 2" stir bars.


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EAT GETS SHIT DONE


:flame::chief:JOIN THE POW WOW:chief::flame:

Edited by eatyualive (03/13/15 09:10 PM)

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OfflinehamloafM
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Re: Avoid The Noid With LC's [Re: eatyualive]
    #21409556 - 03/15/15 12:51 AM (9 years, 10 months ago)

Knock (no pun intended) the dust off that old stiplate, eats, or you're like v.

I have a thing.
1 inch stirbars go in pint size jars.



3.5cm, circular go into quart sized jars.





2 inch stirbars go into gallon sized LC media vessels.



3 attempts with gallon sized media vessels, and still no luck.
It'll happen.
Just gotta' keep going.

You're going to absolutely LOVE GWLC's.
Like The Men's Warehouse.
"I guarantee it"
:lol:



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How I Get Stuff done. - My Reference Guide. - My Grows.

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InvisibleAbshroom
Cause he can


Registered: 06/05/14
Posts: 810
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Re: Avoid The Noid With LC's [Re: eatyualive]
    #21409848 - 03/15/15 03:15 AM (9 years, 10 months ago)

Quote:

eatyualive said:
im gonna give your grain liquid cultures a go pretty soon. im making up some plates in the next week or so to start some things off. first i have to soak some grain. its been close to 15 years since ive done that.:lol:

almost time to dust off the old stir plate and stir bars. i have those 2" stir bars.





No need for soaking. I just boil oats for 30mins (no pre soak) and take this water for my lc's and agar pllates. Works fine :shrug:

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Invisibleeatyualive
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Re: Avoid The Noid With LC's [Re: Abshroom]
    #21413491 - 03/15/15 09:43 PM (9 years, 10 months ago)

Quote:

Abshroom said:
Quote:

eatyualive said:
im gonna give your grain liquid cultures a go pretty soon. im making up some plates in the next week or so to start some things off. first i have to soak some grain. its been close to 15 years since ive done that.:lol:

almost time to dust off the old stir plate and stir bars. i have those 2" stir bars.





No need for soaking. I just boil oats for 30mins (no pre soak) and take this water for my lc's and agar pllates. Works fine :shrug:



i could probably use my hot water bath water from my wbs grain then.


--------------------
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:flame::chief:JOIN THE POW WOW:chief::flame:

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InvisibleAbshroom
Cause he can


Registered: 06/05/14
Posts: 810
Loc: Germany Flag
Re: Avoid The Noid With LC's [Re: eatyualive]
    #21414280 - 03/16/15 12:47 AM (9 years, 10 months ago)

Quote:

eatyualive said:
Quote:

Abshroom said:
Quote:

eatyualive said:
im gonna give your grain liquid cultures a go pretty soon. im making up some plates in the next week or so to start some things off. first i have to soak some grain. its been close to 15 years since ive done that.:lol:

almost time to dust off the old stir plate and stir bars. i have those 2" stir bars.





No need for soaking. I just boil oats for 30mins (no pre soak) and take this water for my lc's and agar pllates. Works fine :shrug:



i could probably use my hot water bath water from my wbs grain then.



Sure, why not? :shrug: just give it a try:cheers:

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OfflinehamloafM
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Re: Avoid The Noid With LC's [Re: Abshroom]
    #21419164 - 03/17/15 04:25 AM (9 years, 9 months ago)

Whichever grain you use.
Be sure it's end-of-the-boil-cycle grain water.
EOTBC grain water has the most nutrients in it.
Has a fair amount of sediment in it as well.




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Invisiblejbaby007
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Posts: 1,031
Re: Avoid The Noid With LC's [Re: hamloaf]
    #21420748 - 03/17/15 01:57 PM (9 years, 9 months ago)

Great info, thanks for sharing! I gotta look for magnetic stir bars. I keep seeing comments about them and wonder if they are any better than just using marbles.  :takingnotes:


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OfflinehamloafM
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Re: Avoid The Noid With LC's [Re: jbaby007]
    #21425174 - 03/18/15 01:57 AM (9 years, 9 months ago)

Magnetic stirbars are of no use on their own.
They must be used in conjunction with a magnetic stir plate.

Stirbar sources;
http://www.ebay.com/itm/111001112069?_trksid=p2055119.m1438.l2649&ssPageName=STRK%3AMEBIDX%3AIT
http://www.ebay.com/itm/221392478671?_trksid=p2055119.m1438.l2649&ssPageName=STRK%3AMEBIDX%3AIT

Stirplate sources;
http://www.ebay.com/itm/Barnstead-Thermolyne-Mirak-S72525-Digital-Magnetic-Stirrer-/291386730741?
http://www.ebay.com/itm/201174853540?_trksid=p2055119.m1438.l2649&ssPageName=STRK%3AMEBIDX%3AIT

I've used marbles, and broken glass.
Wouldn't trade my magnetic stir plate and stirbars in for all the marbles and broken glass in the world.


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Offlinetourrat
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Re: Avoid The Noid With LC's [Re: hamloaf]
    #21425267 - 03/18/15 03:41 AM (9 years, 9 months ago)

Ham have you adjusted your stirring regiment any, as far as speed/length/ frequency?


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OfflinehamloafM
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Re: Avoid The Noid With LC's [Re: tourrat]
    #21426553 - 03/18/15 12:19 PM (9 years, 9 months ago)

Rounding curve with LC's and magnetic stir devices.
Went from stirring once a week to twice.
Found the straight stir bars require a higher rpm speed to generate cyclone (1200).
Circular stirbars only need 700 rpms to generate cyclone.


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