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Just bought me a kg of straw at the pet shop. Well its packed nice and tight so i was wondering if i could just punch a few holes and place the pack in a tub of warm water and pasturize it. And then when its drained just innoc with a piece of agar slant ??
I have though the same with the straw i buy, I don't see why it wouldn't work, but one thing I'll be intererssted to hear back from you is, as it soaks in water, as the straw is very tightly packed, will it expand so much as to split the bag?
Also, you could use an agar piece, but a liquid inocultaion would work better IMO as you could easyerly inject into different point on the block, as you will never be able to shake or mix the straw due to it being so tightly packed.
How is the wedge now ? Is it in a small ziplock baggie, just after transport? If so fill a syringe with 10cc of either distilled water, or bottled spring water, squirt it into the baggie, reseal the baggie, shake and move about the water to mix in the contents. Then draw back into the syringe, and inoculate.(this will also work with a slant)
Its best to take the time to make up a jar of honey water though, if you can wait.
This is assuming you don't have the equipment to make up more cultures and use a full plate.
Sounds like alot of work to not have it colonize. The honey water will work great if you want to grow mold. And the agar won't provide enough innoculation points. The results of cutting corners by not chopping the straw and not using grain spawn is only going to piss you off. Take the time to make the spawn, chop the straw and innoculate at a 10-20% ratio. That's basically 100-200 pounds of spawn (wet), to 1000 pounds of substrate (dry).
It would be great if that would work, but I also think you have alot ofthings going against you. Unless it's spawned quickly straw will have contams, unless it's cut short it will be very slow. On the other hand, since this is a very small bale you may pull it off! especially with liquid myc. I have treated straw with bleach to prevent contams with success, some use h202. These teks may be helpful too. My preference would be to use just a little piece of that agar to start some wbs and save the rest. Good luck & post your results.
Quote: Sounds like alot of work to not have it colonize.
Actually i'm trying to shorten the work. Would save me a lot of time not repacking the straw twice not to mention the mess . Hopefully it works. Thanks for the honey advice tho but i can stil use it to make spawn.
The straw is finely shredded and crushed so i hope that won't be the prob. And i figured if i dunk the log once or twice in peroxidated water it wil be ok. I shot it up with 20 ml of tissuewater on both sides starting in the middle and pulling the syringe back. I'll post back in a few days if it did the trick or not.
Well i looked this morning. Took a nice deep sniff and it smelled ok nothing foul but still no grow either. I think indeed the use of spawn is the only way to go. Think i?ll pasturize again and than use grain spawn. Or perhaps wait a few days!