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taGyo
Strainiac/AMU



Registered: 10/16/14
Posts: 18,802
Loc: Journal Land
Last seen: 6 years, 3 months
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Re: Rye Soak Water Liquid Culture! [Re: hamloaf]
#21253594 - 02/10/15 02:27 PM (9 years, 11 months ago) |
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You're a fucking legend Hamloaf,
-------------------- Gyo's Better Grows
TNF Q&A
AMU Q&A
Dominus fortunae meae sum
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budmanman
OTD Masterbater



Registered: 02/07/07
Posts: 20,495
Loc: PNW
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Re: Rye Soak Water Liquid Culture! [Re: taGyo]
#21253857 - 02/10/15 03:28 PM (9 years, 11 months ago) |
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That might be true, but I touch mine all the time I just wash my hands first.
So far no major or non fixable problem has occurred for me.
God bless mastering the LC.
-------------------- Everything I have ever said is total bogus bs I am full of crud therefore everything I say should never be taken literal.
And I am mentally unstable.
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d0urd3n
Just call me "D"

Registered: 09/15/10
Posts: 5,237
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Re: Rye Soak Water Liquid Culture! [Re: budmanman]
#21274810 - 02/14/15 08:43 PM (9 years, 10 months ago) |
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Re: Rye Soak Water Liquid Culture! [Re: d0urd3n] 1
#21274816 - 02/14/15 08:45 PM (9 years, 10 months ago) |
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looking good!
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d0urd3n
Just call me "D"

Registered: 09/15/10
Posts: 5,237
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Re: Rye Soak Water Liquid Culture! [Re: eatyualive]
#21274852 - 02/14/15 08:54 PM (9 years, 10 months ago) |
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Thanks! Hopefully it's clean. I was thinking about using it soon hopefully there is enough myc in there. The grain jars I innocualted with agar wedges are moving a bit slow. I think it is because I used such small wedges.
My jars seems to move slower than most of you guys. I kind of wonder if two layers of easy felt is restricting the GE too much. I should probably break down and buy some filter disks one of these days. I've already made so many jars with the felt though. I was thinking about throwing some LC in the slow jars.
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hamloaf
Q-dood ®©™√


Registered: 12/23/09
Posts: 24,389
Loc: ation: Based.
Last seen: 17 hours, 47 minutes
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Re: Rye Soak Water Liquid Culture! [Re: eatyualive]
#21274861 - 02/14/15 08:57 PM (9 years, 10 months ago) |
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Cool. Made some end of the boil cycle liquid cuturing medium out of wood chip water for the cultivation of more Pan Stip culture done the right way. 

The mediums were diluted down to a 1:4 ratio of grain water to regular tap water, loaded into their media vessels, and pressure sterilized at 15-17 psi's for 1 hour. The sterilized media vessel surfaces have been sanitized and placed into the lab. The mediums were still a little too hot from being sterilized for inoculation, so the inoculation of Pan Stip live liquid culture is postponed until tomorrow.
Get whatman syringe filters over SFD's for the colonization of liquid mediums. Whatman syringe filters have a design that help prevent the filtering material from becoming wet upon the shake, and pressure sterilization-cycle.
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d0urd3n
Just call me "D"

Registered: 09/15/10
Posts: 5,237
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Re: Rye Soak Water Liquid Culture! [Re: hamloaf]
#21274875 - 02/14/15 09:00 PM (9 years, 10 months ago) |
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Yeah I need to get some of those as well. I figured the felt would do the job until I get some whatmans. I'll keep a syringe or two from this, assuming it is clean. The rest is going to get sprayed all over a nearby horse pasture and massive pile of hpoo.
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hamloaf
Q-dood ®©™√


Registered: 12/23/09
Posts: 24,389
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Re: Rye Soak Water Liquid Culture! [Re: d0urd3n]
#21274956 - 02/14/15 09:30 PM (9 years, 10 months ago) |
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Inoculate agar mediums with a cc of your stored syringe to tell if your culture is clean before expanding upon it. Inoculate more LC, more agar media, or sterilized grain mediums with the agar culture generated from your stored LC cultures if it's clean.
If your culture isn't clean, transfer the clean myce away from the dirty myce onto freshly sterilized agar mediums inside of a clear plastic bag, or SAB until a fully clean culture devoid of contaminates arises.
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d0urd3n
Just call me "D"

Registered: 09/15/10
Posts: 5,237
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Re: Rye Soak Water Liquid Culture! [Re: hamloaf]
#21277987 - 02/15/15 02:24 PM (9 years, 10 months ago) |
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When you guys put LC in your syringe do you leave it full of water if you used the boil technique to sterilize it? Or do you just spray out the sterile water before hand?
Anyways, I think I will test this my LC on agar tonight.
LC hype!
Sorry. Maybe a few of you will get it.
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hamloaf
Q-dood ®©™√


Registered: 12/23/09
Posts: 24,389
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Last seen: 17 hours, 47 minutes
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Re: Rye Soak Water Liquid Culture! [Re: d0urd3n]
#21284075 - 02/16/15 04:54 PM (9 years, 10 months ago) |
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update,
A look at the freshly inoculated with agar wedge, diluted to 4%, Rye GWLC's, ATL#7 - Psilocybe Galindoi. 

A look at the WBS liquid mycelium getting THICK. Colonizing away. 
Made some Woodchip GWLC media the other day. One is in a quart jar, and the other in a gallon sized media vessel.

Both mediums were loaded into the AAx25 and were sterilized at 17psi's for an hour.
Here goes some WoodChip GWLC diluted to 4% that was actually freshly inocualted with live Pan Stip Culture from SporeWorks this afternoon. 

I like the shade of these mediums. 

Also, a look at the Red Milo grain that was inocualted with the undiluted Red Milo GWLC just before being shook. 
Edited by liquidmyce (02/16/15 05:00 PM)
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d0urd3n
Just call me "D"


Registered: 09/15/10
Posts: 5,237
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Re: Rye Soak Water Liquid Culture! [Re: hamloaf]
#21284991 - 02/16/15 07:09 PM (9 years, 10 months ago) |
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Good stuff Ham.
Just filled a syringe with mine and put some drops on agar to test it.
Swirled it as best as I could without getting the filter wet. Don't really see much myc in there, maybe a couple tiny dots. Hopefully that is alright.
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d0urd3n
Just call me "D"


Registered: 09/15/10
Posts: 5,237
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Re: Rye Soak Water Liquid Culture! [Re: d0urd3n]
#21293285 - 02/18/15 12:07 PM (9 years, 10 months ago) |
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Well. Think I am fucked. Panaeolus.
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blazedup
Dirk Diggler


Registered: 06/05/11
Posts: 1,240
Loc: Middle Earth
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Re: Rye Soak Water Liquid Culture! [Re: d0urd3n]
#21294288 - 02/18/15 03:51 PM (9 years, 10 months ago) |
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Nice write up...I will be attempting lc as my next new thing to learn
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invitro

Registered: 05/03/13
Posts: 2,529
Last seen: 1 year, 1 month
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Re: Rye Soak Water Liquid Culture! [Re: blazedup]
#21394611 - 03/11/15 07:08 PM (9 years, 10 months ago) |
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When you guys make your soak water, are you adding coffee? I thought that was pretty standard but I see no mention of it, I read the whole thread just now. Also when you say diluted to 4%, you mean 4 parts soak water to 96 parts water right?
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hamloaf
Q-dood ®©™√


Registered: 12/23/09
Posts: 24,389
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Re: Rye Soak Water Liquid Culture! [Re: invitro]
#21396096 - 03/12/15 05:03 AM (9 years, 10 months ago) |
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Nah, not adding coffee. 1:4, grain water/plain water. Pc for 45 minutes @ 15 psi.
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Re: Rye Soak Water Liquid Culture! [Re: hamloaf]
#21396185 - 03/12/15 05:49 AM (9 years, 10 months ago) |
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badass loaf your bringing back some memories here. even though the last few years it has been heresay to mention liquid cultures prior to that, the boards were full of everyone using liquid cultures and bag spawn. i took a little vacation and came back and things changed big time. i still don't understand many of the things i read on these forums. so back on topic, those jars look fantastic! how many days is your average for the LC to colonize on grain?
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spacechildo
proletarians rise up



Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 7 years, 4 months
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Re: Rye Soak Water Liquid Culture! [Re: hamloaf]
#21396264 - 03/12/15 06:43 AM (9 years, 10 months ago) |
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do you think maybe you used a bit too much in these jars loaf? The growth in general looks good but towards the bottom where liquids collect it looks a bit bacterial to me..
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hamloaf
Q-dood ®©™√


Registered: 12/23/09
Posts: 24,389
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Re: Rye Soak Water Liquid Culture! [Re: spacechildo]
#21396339 - 03/12/15 07:12 AM (9 years, 10 months ago) |
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My LC's look good to me.
The bottom of the jars is where you want the growth to be. You also want the uncolonized section of the media to be see through.
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spacechildo
proletarians rise up



Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 7 years, 4 months
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Re: Rye Soak Water Liquid Culture! [Re: hamloaf]
#21396351 - 03/12/15 07:15 AM (9 years, 10 months ago) |
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not the lc jars. the grain jars.
I forgot the pic I had copied to post in.. here:
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hamloaf
Q-dood ®©™√


Registered: 12/23/09
Posts: 24,389
Loc: ation: Based.
Last seen: 17 hours, 47 minutes
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Re: Rye Soak Water Liquid Culture! [Re: spacechildo]
#21396364 - 03/12/15 07:21 AM (9 years, 10 months ago) |
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Oh, ok. Nah, I just transferred two of those 8 jars into sterile substrate blocks a couple of days ago. Upon breaking up the spawn and opening the jar, the lab instantly became over taken by the sweet smell of freshly colonized mushroom mycelium wafting through the air. The smell of the mycelium was so strong that the alcohol smell that was steadily wafting through the air was immediately replaced.
Edit to add ~ I use 5cc's of thick LC to inoculate my jars with, so the growth you are seeing at the bottom is LC that's ran down the substrate, settled on the bottom, and colonized there.
Edited by liquidmyce (03/12/15 07:30 AM)
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