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InvisiblebodhisattaMDiscordReddit
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Spores Are For Microscopy Only! * 55
    #21365919 - 03/05/15 12:31 PM (8 years, 10 months ago)



I took a medium sized medium darkness cubensis print. it's a little over an inch in diameter.

I was going to try to find out how many spores were on this print, however when I got the whole print in 10mL of water I couldn't break all the clumps up, I tried using some glycerine and an ultrasonic cleaner and I still had quite a bit of clumps, this means I wouldn't get an accurate number if I counted on a counting slide. so I did this instead



I took a very small speck as you can see in the middle of the coverslip on the slide.


this is at 100x magnification brightfield (10x eyepiece and 10x objective)


this is at 400x brightfield (10x eye 40x obj)


this is the top left still at 400x


this is part of the top left at 1000x oil immersion brightfield(10x 100x obj)

that's just one speck from a print, easily 1/1000th of a print and that one single speck could make a 10mL spore syringe easily.

this miniscule speck easily has 10,000,000 spores
if you put that one single speck into a syringe each mL would have 1 million spores.
One million spores per PF cake.
this is why a little goes a long way with spores.

Most vendor syringes could be diluted 10 fold and still work just fine.

After doing all of this I took the cover slip and smooshed out the spores a bit


here at 400x you can a small grouping of spores


and here's some of them at 1000x oil immersion.

if you were to use that single print to make 10 syringes. each syringe would have at least a few hundred million spores and that's conservative.


pulled a thread of rhizomorphic growth from agar and put it on a slide. as you can see there's quite a lot going on in even a small little sliver. here's the elusive clamp connection
More pictures of mycelium and agar plates, a continuation of what you see here

Like what you see? give a +5 rating and I'll give you one back if I haven't already done so.
:cheers:


Edited by Trusted cuItivator (03/26/15 05:50 PM)


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Offlinebw86
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Re: Spores Are For Microscopy Only! [Re: bodhisatta] * 2
    #21365939 - 03/05/15 12:34 PM (8 years, 10 months ago)

I know you weren't really expecting to count spores:cool: and this post is to show people how little of a spore solution they need :yesnod: right?


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OfflineItsScience
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Re: Spores Are For Microscopy Only! [Re: bodhisatta] * 1
    #21365948 - 03/05/15 12:37 PM (8 years, 10 months ago)

Super Cool!  They always say that even if you don't see a super dark print you have millions even in the most invisible part, this just confirms that.  Really cool for those of us w/o microscopes!


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Re: Spores Are For Microscopy Only! [Re: bw86] * 1
    #21365950 - 03/05/15 12:38 PM (8 years, 10 months ago)

damn, nice! a couple  hundred millions should be enough to start a grow or 2! :wink:


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Re: Spores Are For Microscopy Only! [Re: spacechildo] * 1
    #21365955 - 03/05/15 12:39 PM (8 years, 10 months ago)

Nice post!


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InvisiblebodhisattaMDiscordReddit
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Re: Spores Are For Microscopy Only! [Re: bw86] * 1
    #21365959 - 03/05/15 12:40 PM (8 years, 10 months ago)

Quote:

bw86 said:
I know you weren't really expecting to count spores:cool: and this post is to show people how little of a spore solution they need :yesnod: right?



I was going to count them but I couldn't get every little clump to break up, I was going to use a haemacytometer but with clumps it would be horribly inaccurate.


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Re: Spores Are For Microscopy Only! [Re: ItsScience] * 1
    #21365960 - 03/05/15 12:40 PM (8 years, 10 months ago)

Ballin'


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InvisiblebodhisattaMDiscordReddit
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Re: Spores Are For Microscopy Only! [Re: bodhisatta] * 2
    #21366015 - 03/05/15 12:54 PM (8 years, 10 months ago)

Quote:

bodhisatta said:
Quote:

bw86 said:
I know you weren't really expecting to count spores:cool: and this post is to show people how little of a spore solution they need :yesnod: right?



I was going to count them but I couldn't get every little clump to break up, I was going to use a haemacytometer but with clumps it would be horribly inaccurate.





speaking of


here's yeast on a counting slide I took a picture of



yeast and cube spores are about the same size so counting them wouldn't have been a problem if I could have got it to homogenize and break up all the clumps


Edited by Trusted cuItivator (03/05/15 01:20 PM)


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Re: Spores Are For Microscopy Only! [Re: Sagescruffy] * 1
    #21366048 - 03/05/15 01:02 PM (8 years, 10 months ago)

This is awesome. The pictures are awesome. You are awesome.


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Re: Spores Are For Microscopy Only! [Re: Monty514] * 1
    #21366067 - 03/05/15 01:06 PM (8 years, 10 months ago)

Good stuff! :hellyeah:


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Offlinebw86
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Re: Spores Are For Microscopy Only! [Re: Monty514] * 1
    #21366068 - 03/05/15 01:06 PM (8 years, 10 months ago)

so you are saying yes you were going to try and count all the spores in a spore print?
I understand using haemocytometer but that's a square mm. AN entire print would take for ever to count.


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InvisiblebodhisattaMDiscordReddit
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Re: Spores Are For Microscopy Only! [Re: bw86] * 1
    #21366075 - 03/05/15 01:08 PM (8 years, 10 months ago)

Quote:

bw86 said:
so you are saying yes you were going to try and count all the spores in a spore print?
I understand using haemocytometer but that's a square mm. AN entire print would take for ever to count.



that's not how it works.


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Offlinebw86
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Re: Spores Are For Microscopy Only! [Re: bodhisatta] * 1
    #21366096 - 03/05/15 01:13 PM (8 years, 10 months ago)

bro there is no way you were gona count an entire spore sprint.Thank you for the useful pictures you posted to help people understand...

every spore print is a different size anyway and drop different amounts you can easily get 4 prints from one cap.
im not here to argue, i thanked you multiple times, i like you as a person and don't want to start some petty feud


Edited by bw86 (03/05/15 01:19 PM)


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OfflineChem-4 OG
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Re: Spores Are For Microscopy Only! [Re: bodhisatta] * 1
    #21366098 - 03/05/15 01:13 PM (8 years, 10 months ago)

Quote:

bodhisatta said:


I took a medium sized medium darkness cubensis print. it's a little over an inch in diameter.

I was going to try to find out how many spores were on a single print, however when I got the whole print in 10mL of water I couldn't break all the clumps up, I tried using some glycerine and an ultrasonic cleaner and I still had quite a bit of clumps, this means I wouldn't get an accurate number if I counted on a counting slide. so I did this instead



I took a very small speck as you can see in the middle of the coverslip on the slide.


this is at 100x magnification brightfield (10x eyepiece and 10x objective)


this is at 400x brightfield (10x eye 40x obj)


this is the top left still at 400x


this is part of the top left at 1000x oil immersion brightfield(10x 100x obj)

that's just one speck from a print, easily 1/1000th of a print and that one single speck could make a 10mL spore syringe easily.

this miniscule speck easily has 10,000,000 spores
if you put that one single speck into a syringe each mL would have 1 million spores.
One million spores per PF cake.
this is why a little goes a long way with spores.

Most vendor syringes could be diluted 10 fold and still work just fine.

After doing all of this I took the cover slip and smooshed out the spores a bit


here at 400x you can a small grouping of spores


and here's some of them at 1000x oil immersion.

if you were to use that single print to make 10 syringes. each syringe would have at least a few hundred million spores and that's conservative.

Like what you see? give a +5 rating and I'll give you one back if I haven't already done so.
:cheers:




Since I can't afford a microscope of my own yet, I very much appreciate these photos and I will be making note of them.

I hope to see more of these photos with your interpretation of whats going on. :bow2:


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Re: Spores Are For Microscopy Only! [Re: bodhisatta] * 1
    #21366100 - 03/05/15 01:15 PM (8 years, 10 months ago)

GREAT STUFF. a microscope is in my future for sure!


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InvisiblebodhisattaMDiscordReddit
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Re: Spores Are For Microscopy Only! [Re: Hashish] * 1
    #21366111 - 03/05/15 01:18 PM (8 years, 10 months ago)

just for you BW

Quote:

bw86 said:
bro there is no way you were gona count an entire spore sprint.Thank you for the useful pictures you posted to help people understand...

every spore print is a different size anyway and drop different amounts you can easily get 4 prints from one cap.
im not here to argue, i thanked you multiple times, i like you as a person and don't want to start some petty feud




I'm not starting a feud you're just getting confused because you don't know how a counting slide works. yes you can count the whole print, no you don't physically count the whole print. and yes every print is different, I was just counting MY print as an example. it's only representative of itself not any other print unless that other print is the same size just as dark and even then...


here's yeast on a counting slide I took a picture of

I counted 48 viable yeast cells on that middle square there.
that's 1/25th of 1x10-4thml

the yeast sample I put on the counting slide was diluted 1:100
IE 1ml yeast slurry into 9ml of water, shake that up. take 1ml of that solution and put it into 9ml of water. then take some of that and put it on the counting slip. now the yeast you're looking at is diluted 1:100 if you didn't dilute it there would be too many cells to count on the grid. you want between 20-100 cells on each of the squares so you can accurately count them.

so if I got 48 viable cells * 25(cus there's 24 more other squares I didn't count) * 100(dilution factor) * 104th to get back to mL

you get 1.2x109thcells per ml

for example say I scraped that whole entire print into 500ml of water.
I put it on the counting slide and I counted lets just say 50 spores in one of those squares. then I would know that there was ~6.25 trillion spores on that whole print. I couldn't get the spores to un-clump. so I couldn't put them on the counting slide. thus I don't know how many spores are on that print. I'll try it again some other day but there's more than trillions of spores on the print. I would guess 1x1010th as a conservative answer to how many spores are there on the whole print.


Edited by Trusted cuItivator (03/05/15 01:23 PM)


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Invisiblewowimflabbergasted
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Re: Spores Are For Microscopy Only! [Re: bodhisatta] * 1
    #21366121 - 03/05/15 01:21 PM (8 years, 10 months ago)

Errr math  :trippingsheens:


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Offlinebw86
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Re: Spores Are For Microscopy Only! [Re: bodhisatta] * 1
    #21366123 - 03/05/15 01:21 PM (8 years, 10 months ago)

thank you :hatsoff:
but that would still be how  many spores dropped from that print.
not how many spores are in the print, or even an estimate to how many spores are in cube print in general.
i guess im just arguing semantics because you said how"many spores were on a single print" not that print.
:hatsoff: thank you for your contribution.


Edited by bw86 (03/05/15 01:27 PM)


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InvisiblebodhisattaMDiscordReddit
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Re: Spores Are For Microscopy Only! [Re: bw86] * 1
    #21366163 - 03/05/15 01:29 PM (8 years, 10 months ago)

Quote:

bw86 said:
thank you :hatsoff:



so you see I'm not counting the whole print spore by spore. I'm counting a representative part of the print. I put the whole entire print into 500ml of water. then I take a fraction of a drop onto the counting slide. a counting slide works by using a special cover slip only a set volume of liquid is in the space between the cover slip and the slide so you have a known volume in which you're counting cells or spores. you count a small volume then multiply it by your dilution and then multiply it by the volume of the counting slide.

sort of like I take every single person on the planet and load them into the pacific ocean. I take 1000 gallons of pacific ocean and find 5 humans in there. then I multiply that up by the whole volume of the ocean and get an approximate number of people on the whole planet. this is why the sample has to be extremely homogeneous with no clumps.


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Re: Spores Are For Microscopy Only! [Re: bw86] * 1
    #21366176 - 03/05/15 01:32 PM (8 years, 10 months ago)

Quote:

bw86 said:
thank you :hatsoff:
but that would still be how  many spores dropped from that print.
not how many spores are in the print, or even an estimate to how many spores are in cube print in general.
i guess im just arguing semantics because you said how"many spores were on a single print" not that print.
:hatsoff: thank you for your contribution.



I changed the wording of the first post. I was going to count the spores on my print, I scraped the whole entire print off so there was nothing left on the foil. I put the print into 10ml of water then poured that 10ml into a graduated cylinder and brought it up to 500ml (50 syringes for one print basically)

but when I put the sample on the counting slide I could only see clumps of spores not homogenous so I gave up trying to count them.


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Offlinebw86
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Re: Spores Are For Microscopy Only! [Re: bodhisatta]
    #21366202 - 03/05/15 01:34 PM (8 years, 10 months ago)

:seriousthankyou: your a good man. i will not question you in the future :yesnod:

could you print directly onto the counting slide maybe the scraping added to the clumping.


Edited by bw86 (03/05/15 01:36 PM)


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Re: Spores Are For Microscopy Only! [Re: bw86]
    #21366413 - 03/05/15 02:28 PM (8 years, 10 months ago)

If you printed directly onto the counting slide, the print would be too thick to count.


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Re: Spores Are For Microscopy Only! [Re: Psilicon]
    #21366496 - 03/05/15 02:50 PM (8 years, 10 months ago)

Wow man thats pretty crazy, to bad you cant just get one small little spore onto a pin head and drop it, make a spore print last you your whole life haha.


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Re: Spores Are For Microscopy Only! [Re: Psilicon]
    #21366497 - 03/05/15 02:50 PM (8 years, 10 months ago)

and too dense.


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Re: Spores Are For Microscopy Only! [Re: kushroom]
    #21366582 - 03/05/15 03:06 PM (8 years, 10 months ago)

Quote:

kushroom said:
Wow man thats pretty crazy, to bad you cant just get one small little spore onto a pin head and drop it, make a spore print last you your whole life haha.




a print can last a very long time with agar.
Just use a inoculation loop to pick up a few spores and wipe on agar.
Their is a reason agar is highly looked :deemsters:


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Re: Spores Are For Microscopy Only! [Re: Chem-4 OG]
    #21366601 - 03/05/15 03:10 PM (8 years, 10 months ago)

Yeah ive been looking at getting my hands on starting with agar but I havent been able to find all the supplies for a reasonable price or in reasonable distance to me. And all my substitute agars ive tried have failed so bad I dont even wanna try that again haha.


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Re: Spores Are For Microscopy Only! [Re: bodhisatta]
    #21366813 - 03/05/15 03:55 PM (8 years, 10 months ago)

Cool.


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Re: Spores Are For Microscopy Only! [Re: kushroom]
    #21366818 - 03/05/15 03:56 PM (8 years, 10 months ago)

Bodhissata good work...counting the spores is good stuff.
Wonder if you can stain them like yeast for viability....even though there for microscopy use only.  :muahaha:


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Re: Spores Are For Microscopy Only! [Re: Val]
    #21366842 - 03/05/15 03:59 PM (8 years, 10 months ago)

Quote:

Val said:
Bodhissata good work...counting the spores is good stuff.
Wonder if you can stain them like yeast for viability....even though there for microscopy use only.  :muahaha:



spores stay viable for a very long time they're in a vegetative state. yeast on the other hand does produce spores(but brewers yeast rarely does outside of a controlled laboratory environment)

spores probably wouldn't stain too well since they don't have a membrane like a cell does which can take up stain.


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Re: Spores Are For Microscopy Only! [Re: bodhisatta]
    #21366942 - 03/05/15 04:15 PM (8 years, 10 months ago)

Quote:

bodhisatta said:

spores probably wouldn't stain too well since they don't have a membrane like a cell does which can take up stain.




Amyloid spores, of which there are a pretty fair number, will turn blue in iodine because of their starchy coating.


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Re: Spores Are For Microscopy Only! [Re: Psilicon]
    #21366964 - 03/05/15 04:19 PM (8 years, 10 months ago)

Quote:

van der griegen said:
Quote:

bodhisatta said:

spores probably wouldn't stain too well since they don't have a membrane like a cell does which can take up stain.




Amyloid spores, of which there are a pretty fair number, will turn blue in iodine because of their starchy coating.



http://en.wikipedia.org/wiki/Amyloid_%28mycology%29
:thumbup:

cool


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Re: Spores Are For Microscopy Only! [Re: bodhisatta]
    #21371405 - 03/06/15 03:53 PM (8 years, 10 months ago)

after looking at those high magnification pictures and doing some math I would but my bottom line estimate of how many spores there are on that print at

1x10^10 to an upper range of 1x10^12th

10,000,000,000-1,000,000,000,000

somewhere in there is my guess. I'll try to do this again and get a more accurate number but if got to find a way to get spores to not clump together so much, better than sex lube or glycerine does.


Edited by Trusted cuItivator (03/06/15 04:06 PM)


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Re: Spores Are For Microscopy Only! [Re: bodhisatta]
    #21371522 - 03/06/15 04:28 PM (8 years, 10 months ago)

:goat: even more uses for sex lube


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Re: Spores Are For Microscopy Only! [Re: urthtown] * 1
    #21371562 - 03/06/15 04:38 PM (8 years, 10 months ago)

Quote:

bodhisatta said:

10,000,000,000-1,000,000,000,000





:sagan:


Quote:

urthtown said:
:goat: even more uses for sex lube




:sagan:


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Re: Spores Are For Microscopy Only! [Re: Psilicon]
    #21371625 - 03/06/15 04:56 PM (8 years, 10 months ago)

Science!

:awecluster:


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Re: Spores Are For Microscopy Only! [Re: bodhisatta]
    #21371908 - 03/06/15 06:21 PM (8 years, 10 months ago)

Quote:

bodhisatta said:
just for you BW

Quote:

bw86 said:
bro there is no way you were gona count an entire spore sprint.Thank you for the useful pictures you posted to help people understand...

every spore print is a different size anyway and drop different amounts you can easily get 4 prints from one cap.
im not here to argue, i thanked you multiple times, i like you as a person and don't want to start some petty feud




I'm not starting a feud you're just getting confused because you don't know how a counting slide works. yes you can count the whole print, no you don't physically count the whole print. and yes every print is different, I was just counting MY print as an example. it's only representative of itself not any other print unless that other print is the same size just as dark and even then...


here's yeast on a counting slide I took a picture of

I counted 48 viable yeast cells on that middle square there.
that's 1/25th of 1x10-4thml

the yeast sample I put on the counting slide was diluted 1:100
IE 1ml yeast slurry into 9ml of water, shake that up. take 1ml of that solution and put it into 9ml of water. then take some of that and put it on the counting slip. now the yeast you're looking at is diluted 1:100 if you didn't dilute it there would be too many cells to count on the grid. you want between 20-100 cells on each of the squares so you can accurately count them.

so if I got 48 viable cells * 25(cus there's 24 more other squares I didn't count) * 100(dilution factor) * 104th to get back to mL

you get 1.2x109thcells per ml

for example say I scraped that whole entire print into 500ml of water.
I put it on the counting slide and I counted lets just say 50 spores in one of those squares. then I would know that there was ~6.25 trillion spores on that whole print. I couldn't get the spores to un-clump. so I couldn't put them on the counting slide. thus I don't know how many spores are on that print. I'll try it again some other day but there's more than trillions of spores on the print. I would guess 1x1010th as a conservative answer to how many spores are there on the whole print.




So if you can count 2 spores per second and there are 1x1010th on the print it would only take you 1x1010th/(2*60*60*24*365)=159 years. I think you may need some help counting that print. 
:douchewink:


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Re: Spores Are For Microscopy Only! [Re: bodhisatta]
    #21372025 - 03/06/15 06:55 PM (8 years, 10 months ago)

Quote:

bodhisatta said:
after looking at those high magnification pictures and doing some math I would but my bottom line estimate of how many spores there are on that print at

1x10^10 to an upper range of 1x10^12th

10,000,000,000-1,000,000,000,000

somewhere in there is my guess. I'll try to do this again and get a more accurate number but if got to find a way to get spores to not clump together so much, better than sex lube or glycerine does.




Ive heard of jet dry (dishwasher additive) being used for anti-clumping maybe something to look into. My 2 cents.


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Re: Spores Are For Microscopy Only! [Re: bodhisatta]
    #21376457 - 03/07/15 10:49 PM (8 years, 10 months ago)

Quote:

bodhisatta said:


I took a medium sized medium darkness cubensis print. it's a little over an inch in diameter.

I was going to try to find out how many spores were on this print, however when I got the whole print in 10mL of water I couldn't break all the clumps up, I tried using some glycerine and an ultrasonic cleaner and I still had quite a bit of clumps, this means I wouldn't get an accurate number if I counted on a counting slide. so I did this instead



I took a very small speck as you can see in the middle of the coverslip on the slide.


this is at 100x magnification brightfield (10x eyepiece and 10x objective)


this is at 400x brightfield (10x eye 40x obj)


this is the top left still at 400x


this is part of the top left at 1000x oil immersion brightfield(10x 100x obj)

that's just one speck from a print, easily 1/1000th of a print and that one single speck could make a 10mL spore syringe easily.

this miniscule speck easily has 10,000,000 spores
if you put that one single speck into a syringe each mL would have 1 million spores.
One million spores per PF cake.
this is why a little goes a long way with spores.

Most vendor syringes could be diluted 10 fold and still work just fine.

After doing all of this I took the cover slip and smooshed out the spores a bit


here at 400x you can a small grouping of spores


and here's some of them at 1000x oil immersion.

if you were to use that single print to make 10 syringes. each syringe would have at least a few hundred million spores and that's conservative.

Like what you see? give a +5 rating and I'll give you one back if I haven't already done so.
:cheers:




Really cool man thanks! We all should be trading partial prints!


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Re: Spores Are For Microscopy Only! [Re: randoman]
    #21376609 - 03/07/15 11:54 PM (8 years, 10 months ago)

...or swabs. :awesomenod:


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Re: Spores Are For Microscopy Only! [Re: Grey]
    #21376999 - 03/08/15 05:10 AM (8 years, 10 months ago)

great info and pics bodisatta!
how did you take them? and the ones of 25x magnification are great too!!


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Re: Spores Are For Microscopy Only! [Re: mustangbob3]
    #21377363 - 03/08/15 09:05 AM (8 years, 10 months ago)

Quote:

mustangbob3 said:
great info and pics bodisatta!
how did you take them? and the ones of 25x magnification are great too!!



these were all taken on a microscope with a camera and a little 23mm adapter piece for the trinocular port. it's a phase contrast microscope but I took all the pictures in brightfield since the PHX didn't look as nice, PHX looks great for living cells that have organelles you couldn't see as easily with brightfield. I use PHX when I want to try to see vacuoles in yeast to see if they're getting stressed


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Re: Spores Are For Microscopy Only! [Re: bodhisatta]
    #21377400 - 03/08/15 09:15 AM (8 years, 10 months ago)

Ok, Meester Itakemicroscopypictures.
What is PHX?
Do "CDLR" type cameras have adapters, and, or can be used with PHX??


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Re: Spores Are For Microscopy Only! [Re: bodhisatta]
    #21377405 - 03/08/15 09:17 AM (8 years, 10 months ago)

I wish I had access to a lab. Really cool to actually get to see proof of what is preached here all the time.

You inspired me to want a microscope. What should a first timer be looking for? Other than :rofl2:


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Re: Spores Are For Microscopy Only! [Re: hamloaf]
    #21377421 - 03/08/15 09:20 AM (8 years, 10 months ago)

Quote:

Dr_Mcgillicuddy said:
I test the WBS moisture by putting it inside one of my Sub woofers. Turn it up and it it flys out all over the place it is good to collect up and use




This question just came up in the new Q&A. What an answer that is!


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Re: Spores Are For Microscopy Only! [Re: hamloaf]
    #21377422 - 03/08/15 09:21 AM (8 years, 10 months ago)

Quote:

THE_LOAF said:
Ok, Meester Itakemicroscopypictures.
What is PHX?
Do "CDLR" type cameras have adapters, and, or can be used with PHX??



phase contrast microscope but I took all the pictures in brightfield since the PHX didn't look as nice.

for some reason I bet PHX means phase contrasting.

phase contrast is a way a microscope works. the objectives condenser and some other things have to be special for doing phase contrast. but what you see from the eye piece you can take with any old camera.


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Re: Spores Are For Microscopy Only! [Re: randoman]
    #21377426 - 03/08/15 09:22 AM (8 years, 10 months ago)

Quote:

randoman said:
I wish I had access to a lab. Really cool to actually get to see proof of what is preached here all the time.

You inspired me to want a microscope. What should a first timer be looking for? Other than :rofl2:



I've took pretty nice pictures with a scope like that. the thing is you have to use a flash light and it takes a little bit more to setup a good picture with that but it certainly will work as long as you get one with a 10x eyepiece and at least a 10 and 40x objective you'll be fine.


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Re: Spores Are For Microscopy Only! [Re: bodhisatta]
    #21377442 - 03/08/15 09:27 AM (8 years, 10 months ago)

I really had a feeling you were going to say that!:tongue:

I would have never even tried that. I think I have a better microscope that that in the attic somewhere. I bet most noobs also do not realize they dont have to spent a bunch of $$$.

Everyday bodhisatta, everyday. You should do a bodhisatta daily lesson thread. Seriously:seriousthankyou:


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Re: Spores Are For Microscopy Only! [Re: randoman]
    #21377456 - 03/08/15 09:34 AM (8 years, 10 months ago)

Quote:

randoman said:
I really had a feeling you were going to say that!:tongue:

I would have never even tried that. I think I have a better microscope that that in the attic somewhere. I bet most noobs also do not realize they dont have to spent a bunch of $$$.

Everyday bodhisatta, everyday. You should do a bodhisatta daily lesson thread. Seriously:seriousthankyou:



haha, I'm no bodhi, it's just a username.

if you're reading this your the bohdi, I wonder why in life zen is a thing you can chose to learn about. Out of all the endless possibilities for life there's a few things that crystallize into existence, car crashes and zen are among those things. If you chose to learn about it you're doing exactly what you want to be doing, and you're using a world, the air, the ground, some rock in china, an ant climbing a mushroom in a signature on the shroomery, a outer space, and what we feel most importantly a body to do it. and then we go ahead and use the body's brain to have even more thoughts that are in the English language telling us perhaps we are not doing exactly what we want to be doing.

http://www.fairdealtraders.com/products/enlarge/student-microscope.jpg

this is my at home microscope. it works just fine but a mechanical stage would be an upgrade.


Edited by Trusted cuItivator (03/08/15 09:44 AM)


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Re: Spores Are For Microscopy Only! [Re: bodhisatta]
    #21377465 - 03/08/15 09:39 AM (8 years, 10 months ago)

i like that bodhisatta! cheers and thank you for that morning meditation. We should do a daily/morning meditation from quotes of other shroomerites and the daily meditation could be inspiring information on mushrooms or just life in general. Sound idea? cheers ya'll and top of the morning to ya! -TheLostBoys


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Re: Spores Are For Microscopy Only! [Re: TheLostBoys]
    #21377476 - 03/08/15 09:41 AM (8 years, 10 months ago)

Quote:

TheLostBoys said:
i like that bodhisatta! cheers and thank you for that morning meditation. We should do a daily/morning meditation from quotes of other shroomerites and the daily meditation could be inspiring information on mushrooms or just life in general. Sound idea? cheers ya'll and top of the morning to ya! -TheLostBoys



you fire one up and I'll meet you there if you send me a link


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Re: Spores Are For Microscopy Only! [Re: bodhisatta]
    #21377609 - 03/08/15 10:16 AM (8 years, 10 months ago)

I hope this book makes my head spin like what you just wrote did.


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Re: Spores Are For Microscopy Only! [Re: randoman]
    #21485213 - 03/31/15 08:38 PM (8 years, 9 months ago)

Killer photos and post, Bodhi.

Thanks for that.


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Re: Spores Are For Microscopy Only! [Re: DirdyD]
    #21485387 - 03/31/15 09:21 PM (8 years, 9 months ago)

:rockon:


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Re: Spores Are For Microscopy Only! [Re: bodhisatta]
    #21485666 - 03/31/15 10:34 PM (8 years, 9 months ago)

:bow2:


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Re: Spores Are For Microscopy Only! [Re: DirdyD]
    #21485818 - 03/31/15 11:19 PM (8 years, 9 months ago)

here's some bacteria pictures


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Re: Spores Are For Microscopy Only! [Re: bodhisatta]
    #21551762 - 04/15/15 03:59 PM (8 years, 9 months ago)

This is neat, thanks. I could probably shoot a vendor-bought spore syringe into a quart of PC'd water and suck it back up into additional syringes without a loss in viability, huh?


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Re: Spores Are For Microscopy Only! [Re: stareatclouds]
    #21551764 - 04/15/15 04:00 PM (8 years, 9 months ago)

likely


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Re: Spores Are For Microscopy Only! [Re: bodhisatta]
    #21551903 - 04/15/15 04:26 PM (8 years, 9 months ago)

:mushroom2::mushroom2::mushroom2::mushroom2::mushroom2:
Here is five for ya and five more in your ratings man!!!
Great write up! Keep us learning !


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Re: Spores Are For Microscopy Only! [Re: funghi37]
    #21673658 - 05/13/15 07:38 AM (8 years, 8 months ago)

This has been really educational :handth: :handth: Thank you for sharing! :shineon:


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Re: Spores Are For Microscopy Only! [Re: Rosen_Rot]
    #21704388 - 05/20/15 09:52 PM (8 years, 8 months ago)

TL;DR, but if you suspend a cap over a measured amount of water/lube to drop spores you may get less clumping. Idk for sure, but it's what I'd try.


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i recommend common sense and figuring it out.

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Re: Spores Are For Microscopy Only! [Re: bodhisatta]
    #22362604 - 10/11/15 10:35 AM (8 years, 3 months ago)

Very cool and educational... thanks for a great post man...  :hatsoff:


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Re: Spores Are For Microscopy Only! [Re: FreeWorldOrder]
    #22767610 - 01/11/16 08:33 PM (8 years, 18 days ago)

I see what your avatar is now Muwhahahahahaha


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Re: Spores Are For Microscopy Only! [Re: Groo]
    #22767617 - 01/11/16 08:35 PM (8 years, 18 days ago)

ah the clamp connection

it was almost on wikipedia
alan rockefeller put it up it was flagged for removal because there was no permission

so I made a post on here saying YOU CAN USE IT or something like that but it wasn't good enough.



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Re: Spores Are For Microscopy Only! [Re: bodhisatta]
    #22767660 - 01/11/16 08:46 PM (8 years, 18 days ago)

What exactly is the little bulge on the connections? Do they just grow outward or something? And are the little patterns within the myc the nutrients its passing around?


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Re: Spores Are For Microscopy Only! [Re: stareatclouds]
    #22767687 - 01/11/16 08:53 PM (8 years, 18 days ago)

Citation from http://science.jrank.org/pages/2894/Fungi-Basidiomycota-club-fungi.html

Each of the new cells in the secondary mycelium has one haploid nucleus from each parent. This is assured by clamp connections, specialized structures unique to the club fungi. These are loop-like hyphae which connect the cytoplasm of adjacent cells and through which nuclei move during cell division. In particular, during cell division, one nucleus divides directly into the newly formed cell; the other nucleus divides inside the clamp connection and the two daughter nuclei migrate through the clamp connection in opposite directions to the two daughter cells.

Read more: Fungi - Basidiomycota, Club Fungi - Species, Haploid, Mycelium, and Nuclei - JRank Articles http://science.jrank.org/pages/2894/Fungi-Basidiomycota-club-fungi.html#ixzz3wzz7lO97


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Re: Spores Are For Microscopy Only! [Re: Groo]
    #22767753 - 01/11/16 09:06 PM (8 years, 18 days ago)

the clamp is how the nucleus is passed to make dikaryons or our secondary mycelium. each cell in fruiting strains has 2 nuclei. some fungi maybe even cubes during fruiting in the primodria(knots) cells can have way more than 2 nuclei


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Re: Spores Are For Microscopy Only! [Re: bodhisatta]
    #23113606 - 04/13/16 02:45 PM (7 years, 9 months ago)

Very imformative being able to see just how many spores are in a print and just how far they can stretch


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Re: Spores Are For Microscopy Only! [Re: Jahnobi]
    #23116161 - 04/14/16 07:10 AM (7 years, 9 months ago)

:rockon:


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Re: Spores Are For Microscopy Only! [Re: bodhisatta]
    #23116184 - 04/14/16 07:26 AM (7 years, 9 months ago)

this is so cool. and another reason why i would like a microscope.


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Re: Spores Are For Microscopy Only! [Re: bodhisatta]
    #24101966 - 02/19/17 06:14 AM (6 years, 11 months ago)

Awesomeness!


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Re: Spores Are For Microscopy Only! [Re: abductee]
    #24101994 - 02/19/17 06:52 AM (6 years, 11 months ago)

Wow that is fantastic and really brings it into perspective when you see things at that level. Thanks man for spending the time to do this!


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Re: Spores Are For Microscopy Only! [Re: CapnZ]
    #24973325 - 02/07/18 08:41 AM (5 years, 11 months ago)

So I'm on my phone and spent five minutes looking at how to save this thread somehow without resurrecting it, at the bottom border I clicked "favorite" and supposedly it was added to an area called "my home". Well I can't find my home and after ten minutes I'm annoyed and figure this thread is good enough it deserves a resurrect and maybe someone can teach me how to not get annoyed and still fail next time.

Sorry mods I gotta reference this post in another post rn


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Re: Spores Are For Microscopy Only! [Re: Humble Newcomer]
    #24973333 - 02/07/18 08:45 AM (5 years, 11 months ago)



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Re: Spores Are For Microscopy Only! [Re: bodhisatta]
    #24973344 - 02/07/18 08:50 AM (5 years, 11 months ago)

Thanks bodhi


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Re: Spores Are For Microscopy Only! [Re: Humble Newcomer]
    #24973351 - 02/07/18 08:55 AM (5 years, 11 months ago)

Finally found it. Click "account" under your name. I kept clicking my name thinking I had to go thru the profile.

In case others are site illiterate


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InvisibleLizardWizard
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Re: Spores Are For Microscopy Only! [Re: bodhisatta]
    #25391992 - 08/16/18 09:45 AM (5 years, 5 months ago)

Have you tried freezing and thawing to unclump spores?


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InvisiblebodhisattaMDiscordReddit
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Re: Spores Are For Microscopy Only! [Re: LizardWizard]
    #25392173 - 08/16/18 11:22 AM (5 years, 5 months ago)

Nope


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InvisibleLizardWizard
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Re: Spores Are For Microscopy Only! [Re: bodhisatta]
    #25392274 - 08/16/18 12:19 PM (5 years, 5 months ago)

Might be worth a shot if you ever need to again..


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OfflineOctopus8
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Re: Spores Are For Microscopy Only! [Re: bodhisatta]
    #25875745 - 03/15/19 11:59 AM (4 years, 10 months ago)

Where's the Bod's Easy AF Syringe Dilution TEK??

Could you just take a half used syringe, suck up the remaining space with distilled / boiled water, shake and use it for inoculation and get the same results?

:syringe::syringe::syringe:


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Re: Spores Are For Microscopy Only! [Re: Octopus8]
    #25875932 - 03/15/19 01:30 PM (4 years, 10 months ago)

Didn't think I needed to make a tek for something that easy lol. This thread implies your plan would work.


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OfflineOctopus8
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Re: Spores Are For Microscopy Only! [Re: bodhisatta]
    #25876210 - 03/15/19 03:45 PM (4 years, 10 months ago)

We'll see! Just knocked 10 1/2 pints.

I really need to get into agar...g2g...a PC...I hate rolling the dice with MS.

Thanks for all your guidance and wisdom, much appreciated!  :bow2:


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OfflineEp1429
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Re: Spores Are For Microscopy Only! [Re: bodhisatta]
    #25876221 - 03/15/19 03:50 PM (4 years, 10 months ago)

Quote:

bodhisatta said:
Didn't think I needed to make a tek for something that easy lol. 




I’ve seen posts asking if they were boiling water correctly, you can’t honestly be surprised.


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