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OfflineTheBigSlug
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Dropped alcohol pad on the floor!
    #21364663 - 03/05/15 05:38 AM (9 years, 2 months ago)

This is my first post. I might be paranoid but I don't know. I searched around on here and couldn't find anything about my problem. I think I completely screwed my inoculation. I had a sponge soaking in 91% alc in a small Tupperware bowl. I was using it to wipe down the needle in between jars. I flamed the needle til red hot and then wiped it with the sponge after wringing the alc out of it.

...then I dropped the sponge in the floor. I guess in the excitement and impatience of my first ever innoc, I just hurried up and picked it up and soaked it back In the alcohol and wrung it out, flamed
My needle again and shot up the second jar. Then, as I pulled my needle out it bent at almost a 90 degree angle :frown:. And then i dropped the sponge again!! Sads and mads were had for sure but I picked it up off the ground and soaked it in alc again and flamed the needle once more and innocd my last jar. What I want to know is did I completely screw it up or do I at least have a chance?

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Invisibleblindingleaf
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Re: Dropped alcohol pad on the floor! [Re: TheBigSlug]
    #21364673 - 03/05/15 05:46 AM (9 years, 2 months ago)

ur gonna have to crawl before u can walk.
its ok that u messed up.

don't wipe with iso after flaming.  wipe before flaming, get it red hot, then count to 10, and inject.  flame sterilization is more reliable than alcohol sanitation in this hobby.

use paper towels or cotton swabs.  sponges are nasty over time, and too expensive for one time use.

i think ur jars have a chance though.


--------------------
A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

The whole is greater than the sum of its parts

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OfflineGreenRabbit
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Re: Dropped alcohol pad on the floor! [Re: blindingleaf]
    #21364745 - 03/05/15 06:29 AM (9 years, 2 months ago)

If I was you I'd just run another set next week. Not that your jars are doomed but now that you learned what not to do you'll have a better time growing.

Definitely have more than a dozen jars and an extra Fruiting Chamber though if you're serious about growing.

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OfflineBugler Boy
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Re: Dropped alcohol pad on the floor! [Re: TheBigSlug]
    #21364756 - 03/05/15 06:35 AM (9 years, 2 months ago)

Quote:

TheBigSlug said:
I think I completely screwed my inoculation. I had a sponge soaking in 91% alc in a small Tupperware bowl. I was using it to wipe down the needle in between jars. I flamed the needle til red hot and then wiped it with the sponge after wringing the alc out of it.
?




You're doing it backwards... Flame > alcohol. You sterilized the needle with the flame, don't get it dirty again wiping it through the sponge. Also use soaked paper towels instead of a sponge.

I bet you're doing PF, the only reason to wipe a needle after the first flame is if verm sticks to it or something, otherwise just keep flaming in between jars and cut out the alcohol wipe


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The mushroom speaks: '"I am old, older than thought in your species, which is itself fifty times older than your history. Though I have been on earth for ages I am from the stars. My home is no one planet, for many worlds scattered through the shining disc of the galaxy have conditions which allow my spores an opportunity for life... How the hypercommunication mode operates is a secret which will not be lightly given to man. But the means should be obvious: it is the occurrence of psilocybin and psilocin in the biosynthetic pathways of my living body that opens for me and my symbiots the vision screens to many worlds"

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InvisiblebodhisattaMDiscordReddit
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Re: Dropped alcohol pad on the floor! [Re: Bugler Boy]
    #21364852 - 03/05/15 07:25 AM (9 years, 2 months ago)

Alcohol first then Flame.
alcohol is a sanitizer
flame is a sterilizer.

don't move backwards

also 91% alcohol is less effective than 70% at killing contamination.

alcohol works by denaturing proteins, it can't denature proteins as easily at 91% because the osmotic pressure the cells don't want to let the alcohol in. diluted down to 70% makes the alcohol more permeable to cell walls so they can go about denaturing everything inside.

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OfflineGreenRabbit
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Re: Dropped alcohol pad on the floor! [Re: bodhisatta]
    #21365198 - 03/05/15 09:25 AM (9 years, 2 months ago)

Quote:

bodhisatta said:
alcohol works by denaturing proteins, it can't denature proteins as easily at 91% because the osmotic pressure the cells don't want to let the alcohol in. diluted down to 70% makes the alcohol more permeable to cell walls so they can go about denaturing everything inside.




Didn't know that. Cool, so the 99% is just for cleaning bongs eh?

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Offlineganjfather
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Re: Dropped alcohol pad on the floor! [Re: GreenRabbit]
    #21365300 - 03/05/15 09:48 AM (9 years, 2 months ago)

Quote:

GreenRabbit said:
Quote:

bodhisatta said:
alcohol works by denaturing proteins, it can't denature proteins as easily at 91% because the osmotic pressure the cells don't want to let the alcohol in. diluted down to 70% makes the alcohol more permeable to cell walls so they can go about denaturing everything inside.




Didn't know that. Cool, so the 99% is just for cleaning bongs eh?




or extracting things :rocket:


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InvisiblebodhisattaMDiscordReddit
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Re: Dropped alcohol pad on the floor! [Re: ganjfather]
    #21365846 - 03/05/15 12:16 PM (9 years, 2 months ago)

which if you're extracting from cubes doesn't work the actives in cubes are soluble in water

save the 91 and 99% for bongs

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Offlinekushroom
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Re: Dropped alcohol pad on the floor! [Re: bodhisatta]
    #21365884 - 03/05/15 12:23 PM (9 years, 2 months ago)

Shit a bit of rubbing alch plus a bit of rock salt will get your bong clean quicker than anything!


--------------------
:/



All submitted posts are by Someone Who Isn't Me (SWIM) -  and in any event are works of pure fiction or outright lies.  Any information, statement, or assertion contained therein should be considered pure unadulterated fictitious lies.

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OfflineItsScience
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Re: Dropped alcohol pad on the floor! [Re: kushroom]
    #21365916 - 03/05/15 12:31 PM (9 years, 2 months ago)

Ugh, I just bought a gallon of 90% a couple of weeks ago.  God damn failed logic.  Time to go buy some DIH20!

Thanks for the bit of wisdom!

For OP Slug, don't get discouraged, we all do stupid shit sometimes, especially when we are starting out.  Since you will no longer be using a sponge, I typically fold about 5 taper towels into squares (for a full sheet, make it 1/4 it's original size), put them in a stack and douse the stack with alcohol.  That way you can grab one and clean your gloves and other bits where you are working, then toss it to the side and grab new towels from the stack as necessary.  It's probably redundant and useful, but that just increases cleanliness.


--------------------
-SCIENCE

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InvisiblebodhisattaMDiscordReddit
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Re: Dropped alcohol pad on the floor! [Re: ItsScience]
    #21365995 - 03/05/15 12:50 PM (9 years, 2 months ago)

you can dilute 91% down into 70%

use filtered tap water or distilled. tap works fine too but idk if your tap water is as good as my tap water

C1V1=C2V2
that's the dilution equation

(3785.41mL)(0.91)=(  ?  )(0.70)

?=4921.03(1.3 gal)

1135.62mL(1.2 quarts) of water is what you have to add to get your gal of 91 to 70% and you'll end up with 1.3gal of 70%

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OfflineItsScience
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Re: Dropped alcohol pad on the floor! [Re: bodhisatta]
    #21366109 - 03/05/15 01:17 PM (9 years, 2 months ago)

Bahaha, Finally I actually get to use something out of my chemisty book at home!  Awesome, thanks! (I'll be honest, it hadn't occurred to me to turn it into a dilution equation.)


--------------------
-SCIENCE

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InvisiblebodhisattaMDiscordReddit
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Re: Dropped alcohol pad on the floor! [Re: ItsScience]
    #21366140 - 03/05/15 01:25 PM (9 years, 2 months ago)


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OfflineTheBigSlug
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Re: Dropped alcohol pad on the floor! [Re: bodhisatta]
    #21366592 - 03/05/15 03:08 PM (9 years, 2 months ago)

Thanks for all the replies guys I greatly appreciate it! And I do plan on running a small set within the next couple of weeks. The ones I just nocd up yesterday were pre sterilized brf jars from outgrow, which came very fast by the way. (Thanks OG!) I have to find some brf before I run some jars of my own,(I have looked everywhere!) which I won't be able to do til next week. But anyways, again thanks for all the help you guys I couldn't do this without this place.

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Offlineganjfather
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Re: Dropped alcohol pad on the floor! [Re: bodhisatta]
    #21366739 - 03/05/15 03:38 PM (9 years, 2 months ago)

Quote:

bodhisatta said:
which if you're extracting from cubes doesn't work the actives in cubes are soluble in water

save the 91 and 99% for bongs





lol definitely not talking about extracting from mushrooms


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OfflineGreenRabbit
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Re: Dropped alcohol pad on the floor! [Re: kushroom]
    #21368636 - 03/05/15 10:46 PM (9 years, 2 months ago)

Quote:

kushroom said:
Shit a bit of rubbing alch plus a bit of rock salt will get your bong clean quicker than anything!




Always leaves a film of resin still... I have a triple perk, and nothing can clean in the middle part. A brush works WAY better than salt for  the top part. Salt is great when the geometry of the bong allows it to impact the walls but mine is too round for salt to benefit much.

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OfflineTheBigSlug
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Re: Dropped alcohol pad on the floor! [Re: GreenRabbit]
    #21368863 - 03/06/15 12:00 AM (9 years, 2 months ago)

Oh and one more thing, the pre sterilized jars I ordered did not have a verm barrier on top. I'm sure this will increase my risk of contam as well. Can anyone give any insight on that?

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OfflineGreenRabbit
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Re: Dropped alcohol pad on the floor! [Re: TheBigSlug]
    #21368867 - 03/06/15 12:00 AM (9 years, 2 months ago)

Quote:

TheBigSlug said:
Oh and one more thing, the pre sterilized jars I ordered did not have a verm barrier on top. I'm sure this will increase my risk of contam as well. Can anyone give any insight on that?




If they came with a Safety Filter Disk its fine

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