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OfflinePreacher
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Registered: 01/06/15
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Loc: Arizona
Last seen: 5 years, 25 days
Help with my first agar attempt please.
    #21358758 - 03/03/15 10:44 PM (9 years, 10 months ago)

Hello all!  I am a long time reader but new to posting...
Anyway, I have some experience growing and have so far gone from spore syringe to LC.  I want to expand my scope and give agar a go.  Since I am using a glove box, I decided to use an MEA agar in 1/2 pint short jars with a PF Tek lid for covered by a tyvek disc.  I inoculated a few jars by several different methods (spore print, spore syringe, LC syringe, and live tissue sample.)  Thus far, I have gotten no mycelium growth on the agar.  They have been in my glove box at room temperature for a few days and, so far, no go.
Any thoughts as to why?
Thanks!

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InvisibleSpitballJedi
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Registered: 10/13/12
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Re: Help with my first agar attempt please. [Re: Preacher]
    #21358852 - 03/03/15 11:10 PM (9 years, 10 months ago)

spores can take several days to germinate.

Live culture or tissue should show visible growth within a few days after transfer. It sounds like you may have an issue with the agar.

We need pics and to know your agar prep and recipe.


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OfflinePreacher
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Registered: 01/06/15
Posts: 9
Loc: Arizona
Last seen: 5 years, 25 days
Re: Help with my first agar attempt please. [Re: SpitballJedi]
    #21358950 - 03/03/15 11:44 PM (9 years, 10 months ago)

The recipe I used was a simple MEA I found in this post:
http://www.shroomery.org/5243/What-are-some-good-agar-media-recipes
10g LME
9g Agar agar
500 ml water (I used RO water from the Glacier machine since the tap water here is only good for flushing toilets.)
Loaded media into the jars and PCed for 30 min.
Thanks for your help!
These are my Lion's Mane LC jar and my Shiitake tissue jar.



Edited by Preacher (03/03/15 11:49 PM)

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