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CarryOn



Registered: 02/02/15
Posts: 14
Last seen: 9 years, 8 months
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Traits for selection
#21353035 - 03/02/15 06:01 PM (9 years, 10 months ago) |
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When isolating strains of p. cubensis, which are the traits to look out for? I am eager to experiment with isolating true breeds, but lack a good overview of the traits of this mushroom.
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Hashish
Knowledge is Power



Registered: 12/04/14
Posts: 492
Loc: 2 the Moon
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Re: Traits for selection [Re: CarryOn]
#21353047 - 03/02/15 06:04 PM (9 years, 10 months ago) |
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take a look at the links in my sig for agar information.
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CarryOn



Registered: 02/02/15
Posts: 14
Last seen: 9 years, 8 months
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Re: Traits for selection [Re: Hashish]
#21353062 - 03/02/15 06:09 PM (9 years, 10 months ago) |
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Thanks, etc. I am beginning to get a decent grip on agar work. My knowledge of mycobiology is adequate, that is I know a little about the life cycle of basidomycota, mono-/dikaryon hyphae and meoisis in fruiting bodies. I've been reading up on here on strategies for isolating true breeds. What I am mostly wondering is what traits to select for visually.
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shroomiedoo22
Level II


Registered: 04/19/14
Posts: 298
Last seen: 9 years, 6 months
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Re: Traits for selection [Re: CarryOn]
#21353098 - 03/02/15 06:17 PM (9 years, 10 months ago) |
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Speedy colonization!
I chased that trait, and I will now fruit it and clone my favorite!
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Level II Shroomer Good Vibes
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filthyknees
no coincidence


Registered: 03/08/13
Posts: 6,283
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basically what shroomydoo22 said. Recently in search of a strong culture I made two different agar recipes (lme or potato), each transfer I switch mediums so that which ever growth is fastest after a handful of transfers I can be pretty sure is a strong colonizer.
you said visually I assume this means on agar. I think it's worth mentioning that cloning young pins (can be done off agar) can capture genetics that are vigorous in growth and the important part: almost certainly a good fruiter. where as from spores if fastest growth is selected it may turn out that the result is not the best fruiter but just fast (who wants that?). Always remember to do trials, never all eggs in one basket.
hoping I addressed your question
-------------------- But if you're in a hurry, and really got to go
If you're in a hurry, might have to find out slow
That it's one thing to try and another to fly
You get there quicker just a step at a time
It's one thing to bark, another to bite
The show ain't over till you pack up at night
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,972
Loc: Canada
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Without fruiting each isolate out there is only one trait that can be selected and that's strong healthy colonization. Otherwise you will need to fruit them to see what you have. Fruit them all and keep the best. Usually one in ten is really good.
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CarryOn



Registered: 02/02/15
Posts: 14
Last seen: 9 years, 8 months
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Re: Traits for selection [Re: Pastywhyte]
#21354240 - 03/02/15 10:24 PM (9 years, 10 months ago) |
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Thanks all.
What do you look for in fruiting bodies? Size? Color? Shape?
Edited by CarryOn (03/02/15 10:27 PM)
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CarryOn



Registered: 02/02/15
Posts: 14
Last seen: 9 years, 8 months
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Quote:
filthyknees said:I think it's worth mentioning that cloning young pins (can be done off agar) can capture genetics that are vigorous in growth and the important part: almost certainly a good fruiter. where as from spores if fastest growth is selected it may turn out that the result is not the best fruiter but just fast (who wants that?). Always remember to do trials, never all eggs in one basket.
What do you mean by "young pins" here?
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mushpunx
Fungus Punk



Registered: 04/20/14
Posts: 13,394
Last seen: 2 months, 8 days
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Re: Traits for selection [Re: CarryOn]
#21354374 - 03/02/15 10:57 PM (9 years, 10 months ago) |
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I say start from clone, it will save work and you have a better chance of isolating good fruiters.
But dont forget potency! I prefer to clone from mature clusters so I can save the mushrooms and eat them when I get a chance, i can get an idea of the potency while Im growing out the cultures
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Amateur Mycologists United
AMU Q&A
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filthyknees
no coincidence


Registered: 03/08/13
Posts: 6,283
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Re: Traits for selection [Re: CarryOn]
#21355371 - 03/03/15 08:09 AM (9 years, 10 months ago) |
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By young pins I mean like the first picture in my user gallery (can't find how to paste on mobile sorry). I mean the first very small mushrooms you see start to form. Some look for clusters of mushrooms to clone from to have a better chance at producing clusters from the clone.
As apposed to cloning a mature fruit with its cap open.
-------------------- But if you're in a hurry, and really got to go
If you're in a hurry, might have to find out slow
That it's one thing to try and another to fly
You get there quicker just a step at a time
It's one thing to bark, another to bite
The show ain't over till you pack up at night
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Guardian187
Neophyte


Registered: 11/29/14
Posts: 716
Loc: Center of the Universe
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Re: Traits for selection [Re: mushpunx]
#21355393 - 03/03/15 08:19 AM (9 years, 10 months ago) |
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Quote:
mushpunx said: I say start from clone, it will save work and you have a better chance of isolating good fruiters.
But dont forget potency! I prefer to clone from mature clusters so I can save the mushrooms and eat them when I get a chance, i can get an idea of the potency while Im growing out the cultures
Can't emphasize this enough!
http://www.shroomery.org/forums/showflat.php/Number/19035259
I don't follow everything in the above link, but there is some useful reading in there. I personally don't do grass seed and stick with mini monos out of preference.
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bw86
Doesn't play well with others


Registered: 11/12/06
Posts: 5,976
Loc: 7b
Last seen: 20 minutes, 11 seconds
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my only contribution to this thread is to stress the important of proper labels.With out them it makes no difference if you took a good isolate or not.
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Guardian187
Neophyte


Registered: 11/29/14
Posts: 716
Loc: Center of the Universe
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Re: Traits for selection [Re: bw86]
#21355407 - 03/03/15 08:27 AM (9 years, 10 months ago) |
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Quote:
bw86 said: my only contribution to this thread is to stress the important of proper labels.With out them it makes no difference if you took a good isolate or not.
Great point too. It gets confusing if you don't label well.
I always put the date, "strain", and generation. I just start with Spores -> Agar -> "PE" 1, "PE" 2, ... PE1 -> PE1-1, PE1-2, etc...
If that makes sense haha. Helps me stay organized on the plate and sectors I transferred from.
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CarryOn



Registered: 02/02/15
Posts: 14
Last seen: 9 years, 8 months
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Great stuff Guardian. Violet's write ups are pure gold. Just what I've been looking for!
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Fungus Mountain
Poke-N-Squirt



Registered: 10/16/14
Posts: 1,188
Loc: Front row @ the Freakshow
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Re: Traits for selection [Re: CarryOn]
#21357535 - 03/03/15 05:53 PM (9 years, 10 months ago) |
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Stock up on petri dishes, this shit gets addicting really fast.
-------------------- “Until they became conscious they will never rebel, and until after they have rebelled they cannot become conscious.”
― George Orwell, 1984
"If you can't explain it simply, you don't understand it well enough."
― Albert Einstein
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bw86
Doesn't play well with others


Registered: 11/12/06
Posts: 5,976
Loc: 7b
Last seen: 20 minutes, 11 seconds
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Quote:
Fungus Mountain said: Stock up on petri dishes, this shit gets addicting really fast.
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