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OfflinetaGyo
Strainiac/AMU
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Registered: 10/16/14
Posts: 18,802
Loc: Journal Land Flag
Last seen: 6 years, 3 months
Re: Continuing Contam issue [Re: tripdawg420]
    #21342675 - 02/28/15 11:16 AM (9 years, 10 months ago)

I think he's talking to me? No idea.


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InvisibleGhatti
Totally not a Federal Agent
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Registered: 06/23/14
Posts: 1,733
Re: Continuing Contam issue [Re: taGyo]
    #21356505 - 03/03/15 01:40 PM (9 years, 10 months ago)

So today I ran a batch of bags with no sleeves. When the cycle was over I sat my PC in front of my hood to cool and depressurize.

Once cool I pulled a bag out and immediately ziptied it without opening in any way. (This bag will indicate if sterilization is incomplete)

The next bag I opened and mock innoced it then ziptied it up. (This bag should tell if it is my hood being faulty or my technique)

If neither one of these bags contaminate I will feel confident saying that it is my culture that is weak/dirty

Agree?

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InvisiblebodhisattaMDiscordReddit
Smurf real estate agent
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Registered: 04/30/13
Posts: 61,916
Loc: Milky way
Trusted Cultivator
Re: Continuing Contam issue [Re: Ghatti]
    #21356513 - 03/03/15 01:42 PM (9 years, 10 months ago)

make a few plates and then plate your culture. the only way to be positive is to eliminate the variables. like the fact that maybe 1 in 6 of your grain bags would have gone bad anyway because you're right on the edge of sufficient sterilization.

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InvisibleGhatti
Totally not a Federal Agent
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Registered: 06/23/14
Posts: 1,733
Re: Continuing Contam issue [Re: bodhisatta]
    #21356553 - 03/03/15 01:50 PM (9 years, 10 months ago)

I have several plates growing out at the moment that won't be ready for a week or so. I'll post pics when the time comes. I would think 3 1/2hrs at 15psi would be sufficient for 6 4qt bags though but I'm probably Wong.

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