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OfflinePsilocyBen17
Pin Pornographer


Registered: 10/20/13
Posts: 3,751
Last seen: 2 years, 29 days
Testing LC's on Agar
    #21355856 - 03/03/15 11:07 AM (9 years, 10 months ago)

hey guys.

I have just started mass producing LC syringes so that I have a good stockpile to trade with. In the past I have only used LC's when absolutely necessary, usually on a few jars at a time. I have 10 syringes now and I would like to test them all, so I will be putting a few drops from each syringe on separate agar plates. If the LC is contaminated, will the WHOLE plate turn into a bacterial mess? Or will spots of bacteria show up?

Any advice on testing syringes would be super cool.


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OfflinePsilicon
Really Nice Guy


Registered: 08/26/12
Posts: 7,057
Last seen: 4 years, 1 month
Re: Testing LC's on Agar [Re: PsilocyBen17]
    #21355879 - 03/03/15 11:13 AM (9 years, 10 months ago)

The amount of bacteria you get on your plate is dependent on how bacterial your LC is, but also on the type of bacteria, the temperature and makeup of your agar.  A better thing to look for is where the bacteria comes from.  If you use the hot needle trick to make a hole in your agar to hold a drop of LC, then if you have bacteria at the edge of the plate you know it wasn't your LC.  If it's in the middle, and grows instead of or alongside mycelial growth, you know your LC was bad.


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Agar - what, why and how?  Everything a beginner needs to know.
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Offlinesilverstem
Caps & Stems
Male

Registered: 10/12/13
Posts: 900
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Last seen: 7 years, 5 months
Re: Testing LC's on Agar [Re: Psilicon]
    #21355944 - 03/03/15 11:34 AM (9 years, 10 months ago)

:whathesaid: I like the tri plates with the 3 diff sections for testing stuff like lcs really saves money. But deff  van says if ur not sure about a plate post a pic we will help you out.


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