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Invisible3iRiS9
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Registered: 01/11/13
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Copelandia cyanescens germination on agar. Contaminated?
    #21354576 - 03/02/15 11:56 PM (9 years, 10 months ago)

5 days ago I inoculated 3 dishes with a year old wild Copelandia cyanescens spore print.
The mixture was a weak MEA for spore germination.
I think 10g MEA / 400ml water.

Anyhow, They appeared to be colonizing but it looks pretty funky.
Some sections are growing straight up into the air like a bubble of cottony mycelium!?

I'm guessing it's Trichoderma? 

Pics:


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InvisiblePirateSwazey
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Re: Copelandia cyanescens germination on agar. Contaminated? [Re: 3iRiS9]
    #21354587 - 03/02/15 11:58 PM (9 years, 10 months ago)

The top fuzzy part looks fine but the bottom of the 2nd pic might be sketchy.

Transfer tomorrow or the next day, as soon as you can grab a little piece from there.


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OfflinecronicrMFacebook
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Re: Copelandia cyanescens germination on agar. Contaminated? [Re: PirateSwazey]
    #21354589 - 03/02/15 11:59 PM (9 years, 10 months ago)

:whathesaid:


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Invisible3iRiS9
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Registered: 01/11/13
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Loc: Southwest Florida
Re: Copelandia cyanescens germination on agar. Contaminated? [Re: cronicr]
    #21354736 - 03/03/15 01:24 AM (9 years, 10 months ago)

They're both photos of the same section, same dish. :frown:

The first photo is at an angle, showing how elevated the mycelium is.
Copelandia cyanescens shouldn't exhibit this type of growth, correct?

edit: It's aerial at about 2-3mm high.

Edited by 3iRiS9 (03/03/15 01:38 AM)

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Invisibleblindingleaf
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Re: Copelandia cyanescens germination on agar. Contaminated? [Re: 3iRiS9]
    #21354896 - 03/03/15 04:11 AM (9 years, 10 months ago)



it looks more like u transferred a wedge than swiped a plate.  i dunno why its growing upwards :stars:


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Invisible3iRiS9
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Registered: 01/11/13
Posts: 512
Loc: Southwest Florida
Re: Copelandia cyanescens germination on agar. Contaminated? [Re: blindingleaf]
    #21354967 - 03/03/15 04:58 AM (9 years, 10 months ago)

I know, it looks so weird to me.

I don't really want to transfer it to find out i just isolated a nice contaminant culture. Hahah.

I also tried cloning a store bought shiitake right after inoculating these plates, and the shiitake plates already have sporulating Trichoderma colonies that have aerial mycelium just like this.

Edit: The contaminants I've found look more like Cladosporium or a similar green mold. Not trichoderma ( I need to research more! )

Photos of green contaminant on Shiitake plates:
Colonies apprx. 1/2" diameter




Photos of C. cyanescens plates:






Edited by 3iRiS9 (03/03/15 10:06 AM)

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