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3iRiS9
@psilocybeing



Registered: 01/11/13
Posts: 512
Loc: Southwest Florida
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Copelandia cyanescens germination on agar. Contaminated?
#21354576 - 03/02/15 11:56 PM (9 years, 10 months ago) |
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5 days ago I inoculated 3 dishes with a year old wild Copelandia cyanescens spore print. The mixture was a weak MEA for spore germination. I think 10g MEA / 400ml water.
Anyhow, They appeared to be colonizing but it looks pretty funky. Some sections are growing straight up into the air like a bubble of cottony mycelium!?
I'm guessing it's Trichoderma?
Pics:
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PirateSwazey


Registered: 12/12/12
Posts: 2,993
Loc: Here, Now
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Re: Copelandia cyanescens germination on agar. Contaminated? [Re: 3iRiS9]
#21354587 - 03/02/15 11:58 PM (9 years, 10 months ago) |
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The top fuzzy part looks fine but the bottom of the 2nd pic might be sketchy.
Transfer tomorrow or the next day, as soon as you can grab a little piece from there.
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cronicr



Registered: 08/07/11
Posts: 62,905
Loc: Van Isle
Last seen: 1 hour, 2 minutes
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Re: Copelandia cyanescens germination on agar. Contaminated? [Re: PirateSwazey]
#21354589 - 03/02/15 11:59 PM (9 years, 10 months ago) |
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-------------------- It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor
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3iRiS9
@psilocybeing



Registered: 01/11/13
Posts: 512
Loc: Southwest Florida
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Re: Copelandia cyanescens germination on agar. Contaminated? [Re: cronicr]
#21354736 - 03/03/15 01:24 AM (9 years, 10 months ago) |
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They're both photos of the same section, same dish. 
The first photo is at an angle, showing how elevated the mycelium is. Copelandia cyanescens shouldn't exhibit this type of growth, correct?
edit: It's aerial at about 2-3mm high.
Edited by 3iRiS9 (03/03/15 01:38 AM)
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blindingleaf
blue collar underworld


Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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Re: Copelandia cyanescens germination on agar. Contaminated? [Re: 3iRiS9]
#21354896 - 03/03/15 04:11 AM (9 years, 10 months ago) |
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it looks more like u transferred a wedge than swiped a plate. i dunno why its growing upwards
-------------------- A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!
The whole is greater than the sum of its parts
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3iRiS9
@psilocybeing



Registered: 01/11/13
Posts: 512
Loc: Southwest Florida
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Re: Copelandia cyanescens germination on agar. Contaminated? [Re: blindingleaf]
#21354967 - 03/03/15 04:58 AM (9 years, 10 months ago) |
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I know, it looks so weird to me.
I don't really want to transfer it to find out i just isolated a nice contaminant culture. Hahah.
I also tried cloning a store bought shiitake right after inoculating these plates, and the shiitake plates already have sporulating Trichoderma colonies that have aerial mycelium just like this.
Edit: The contaminants I've found look more like Cladosporium or a similar green mold. Not trichoderma ( I need to research more! )
Photos of green contaminant on Shiitake plates: Colonies apprx. 1/2" diameter



Photos of C. cyanescens plates:




Edited by 3iRiS9 (03/03/15 10:06 AM)
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