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Registered: 03/08/03
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Do i got a problem here?????
    #2135428 - 11/25/03 06:29 PM (13 years, 10 days ago)

I innoculated my wbs quart jars sunday afternoon, and left them at my friends house. He left all 11 of them in the trunk of his car ( the past two nights have been a low of 30 degrees!) so today I went to go check on them and noticed NO growth in 2 todays....do you think they are some blomers, since now they are in room temp. or do you think the cold weather could have killed all chance of mycelium growth???

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Re: Do i got a problem here????? [Re: rfick]
    #2135440 - 11/25/03 06:34 PM (13 years, 10 days ago)

It's possible it could have but I doubt it.. The spores haven't germinated yet and are usualy stored in cold places, they probably won't show any signs of germination for a few more days if they will be ok, wait another 5 days or so and see if you notice any growth by then. Try and keep them around 85 degrees. It probably would slow down growth but not kill them becuase they haven't been exposed to the cold long enough in my opinion.


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Re: Do i got a problem here????? [Re: Cloud9]
    #2135490 - 11/25/03 06:52 PM (13 years, 10 days ago)

It depends. If the water inside the jars actually froze, chances are a lot of spores were killed, but I doubt all of them were. It may take a while to see signs of growth -- it depends on how many spores were in there in the first place. If there were very few they might be fucked....

If the water in the jars didn't actually freeze you're fine, and if the low temps were only 30, there's a chance they didn't quite get there.

The problem is that the crystalline structure of water has sharp edges and can cut (lyse) the cells. The only thing you can do is wait and see what happens -- inoculate more jars in the meantime if you can....


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Re: Do i got a problem here????? [Re: micro]
    #2136201 - 11/25/03 11:06 PM (13 years, 10 days ago)

Two days is way too soon to see growth anyway. Wait two weeks before you decide that they are dead. As long as they didn't get completely frozen, they will be fine.

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Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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