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OfflineChowder
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Is this agar myc still viable? [pics]
    #21353445 - 03/02/15 07:24 PM (9 years, 10 months ago)





I made these for my first agar attempt. Now I have 12 beautiful fully colonized rye jars. Thrilled, never going back to spores again!

I inoculated these from spores on the 11th of Jan. Fully colonized the plates on the 28th (is this normal colonzation time for 20-22C?)

Now, I have lots of leftover plates, and instead of getting rid of them, I just left them on the window sill, opening them a few times, wondering whether they will fruit. I was hoping to see some little tiny cute fruits.

However, today, 2nd of March, they all look exactly the same, like the one in the pictures - maybe a bit dry. Can I use them for my next batch of rye jars?

How could I get fruits on the agar cups? (just for the lolz)

Also, is there any benefit on transferring a bit of this myc to more agar cups? Or is it too late for isolation?


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Edited by Chowder (03/02/15 07:27 PM)

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InvisibleMr. Alien
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Re: Is this agar myc still viable? [pics] [Re: Chowder]
    #21353471 - 03/02/15 07:29 PM (9 years, 10 months ago)

Did you open that container in open air?

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OfflineChowder
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Re: Is this agar myc still viable? [pics] [Re: Mr. Alien]
    #21353475 - 03/02/15 07:30 PM (9 years, 10 months ago)

Yes.


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InvisibleMr. Alien
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Re: Is this agar myc still viable? [pics] [Re: Chowder]
    #21353476 - 03/02/15 07:30 PM (9 years, 10 months ago)

:facepalm3:

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OfflineChowder
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Re: Is this agar myc still viable? [pics] [Re: Chowder]
    #21353484 - 03/02/15 07:32 PM (9 years, 10 months ago)

So should I start with spores from scratch?

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InvisibleGuardian187
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Re: Is this agar myc still viable? [pics] [Re: Mr. Alien]
    #21353486 - 03/02/15 07:32 PM (9 years, 10 months ago)

Quote:

Mr. Alien said:
:facepalm3:




Haha. Yeah not a good idea to open outside a SAB or flow hood

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OfflineChowder
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Re: Is this agar myc still viable? [pics] [Re: Chowder]
    #21353618 - 03/02/15 07:56 PM (9 years, 10 months ago)

So what about the other questions I asked?
Thanks!

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OfflinetheBitterBuffalo00
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Re: Is this agar myc still viable? [pics] [Re: Chowder]
    #21353653 - 03/02/15 08:02 PM (9 years, 10 months ago)

Do grain to grain transfers or make more petris with the old agar (which is fine) or grain to petri i guess


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OfflineChowder
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Re: Is this agar myc still viable? [pics] [Re: Chowder]
    #21353679 - 03/02/15 08:06 PM (9 years, 10 months ago)

Grain to petris, wow, never thought of that. I presume it's much quicker...
Should I just grab a grain with a sterile tool, or what? Shall I do this just before spawning my grain?

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InvisibleMr. Alien
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Re: Is this agar myc still viable? [pics] [Re: Chowder]
    #21353710 - 03/02/15 08:12 PM (9 years, 10 months ago)

It looks dry and i don't think you will get a mushroom growing from it because of that reason. Never open any plate in open air or they will contam. Never let them fully colonize, refrigerate them before. Room temperature is perfect for colonization.

I would try to transfer a piece of myc from that culture and try to clean it on agar if you want to save that culture. You can also start over from spores if you don't want to mess with contams isolating healthy myc from them... germinate some spore on agar, do 2-3 transfers from the original spore plate to guarantee a clean multi spore culture.

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OfflineChowder
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Re: Is this agar myc still viable? [pics] [Re: Chowder]
    #21353736 - 03/02/15 08:19 PM (9 years, 10 months ago)

Mr.Alien, thanks! Any advice for grain > agar?

I'm not particularly interested in keeping this culture. I just wanted to inoculate tomorrow!, not in a week.

I could try G2G I guess, but I want to use my already colonized grains for spawning.

Could I get these plates to fruit?

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Invisiblefilthyknees
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Re: Is this agar myc still viable? [pics] [Re: Chowder]
    #21353785 - 03/02/15 08:32 PM (9 years, 10 months ago)

if alien doesn't reply by tomorrow here is an easy solution: spawn your grain tomorrow. after breaking up your spawn save one jar (the fastest one), wipe it down and place in your sab with receiving plates, open the jar and transfer a grain per dish, seal up your plates and proceed to spawning your tub with the remaining 99.8% of the grain left over :mushroom2:


--------------------
But if you're in a hurry, and really got to go
If you're in a hurry, might have to find out slow
That it's one thing to try and another to fly
You get there quicker just a step at a time
It's one thing to bark, another to bite
The show ain't over till you pack up at night

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InvisiblePastywhyteMDiscord
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Re: Is this agar myc still viable? [pics] [Re: filthyknees]
    #21353815 - 03/02/15 08:40 PM (9 years, 10 months ago)

Quote:

filthyknees said:
if alien doesn't reply by tomorrow here is an easy solution: spawn your grain tomorrow. after breaking up your spawn save one jar (the fastest one), wipe it down and place in your sab with receiving plates, open the jar and transfer a grain per dish, seal up your plates and proceed to spawning your tub with the remaining 99.8% of the grain left over :mushroom2:




:whathesaid: an easy way to get a lot of inoculate. Its also a good way to isolate as you can often see sectoring after a transfer or two from the grain plate. Much quicker than the dozen transfers it would normally take.

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InvisibleMr. Alien
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Re: Is this agar myc still viable? [pics] [Re: Chowder]
    #21353853 - 03/02/15 08:51 PM (9 years, 10 months ago)

Quote:

Chowder said:
Mr.Alien, thanks! Any advice for grain > agar?

I'm not particularly interested in keeping this culture. I just wanted to inoculate tomorrow!, not in a week.

I could try G2G I guess, but I want to use my already colonized grains for spawning.

Could I get these plates to fruit?




Grain2Agar to save a MS culture just in case, use the remaining spawn to inoculate some bulk substrate, fruit it, choose the fastest pin from a cluster and drop it in agar.

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OfflineChowder
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Re: Is this agar myc still viable? [pics] [Re: Chowder]
    #21353944 - 03/02/15 09:10 PM (9 years, 10 months ago)

Shall I use a flame-sterilized tweezer for this purpose?
Thanks

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Offlinemushpunx
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Re: Is this agar myc still viable? [pics] [Re: Chowder]
    #21354186 - 03/02/15 10:13 PM (9 years, 10 months ago)

Quote:

Chowder said:
Shall I use a flame-sterilized tweezer for this purpose?
Thanks





Could, Id use a scalpel.


  You dont want to let the whole plate colonize man, as soon as you see germination immediatly transfer. Then transfer a few more times before you put it to grain


--------------------

Amateur Mycologists United
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InvisibleMr. Alien
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Re: Is this agar myc still viable? [pics] [Re: Chowder]
    #21354191 - 03/02/15 10:14 PM (9 years, 10 months ago)

Sterilize some half nutrient agar. Use the same agar agar amount (10g per 500ml of water) but use half the nutrients in your agar mix, this will help to encourage myc rapid growth in it's search for nutrients and contams won't thrive as easy in a nutrient weak agar.

Sewing needle on the pin cap, cut the pin with your blade and drop it on your weak agar plate inside the SAB. I also like to sterilize 3 pint jars of water and sumerge the pin in those jars before dropping it on the agar plates. This will help to dilute all the contams on the exterior of the pin into the sterile water baths.

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InvisibleNumeroEno
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Re: Is this agar myc still viable? [pics] [Re: mushpunx]
    #21354222 - 03/02/15 10:20 PM (9 years, 10 months ago)

Everyone has given good advice ITT. If it were me I would transfer that to a few new plates and clean it up. I have no idea how to get an agar plate to pin. I had one like that and I took the lid off and covered the whole top of the plate with parafilm. It still didn't pin. It just dried out :shrug:


--------------------

:gd_icon:  Let it grow! Let it grow! Greatly yield!  :gd_icon:
What shall we say, shall we call it by a name
As well to count the angels dancing on a pin
Water bright as the sky from which it came
And the name is on the earth that takes it in

DOG FOOD AGAR

MY ELECTRIC INOCULATION LOOP

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Offlinemushpunx
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Re: Is this agar myc still viable? [pics] [Re: Mr. Alien]
    #21354229 - 03/02/15 10:21 PM (9 years, 10 months ago)

^ thats a neat trick Alien!

Do you think there is a difference in the clone between a cloned pin or by letting the pin mature and making note of it and then taking a clone?

It should be the same clone yea?


--------------------

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InvisibleMr. Alien
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Re: Is this agar myc still viable? [pics] [Re: mushpunx]
    #21354244 - 03/02/15 10:25 PM (9 years, 10 months ago)

Yeah it should be the same clone, but the pin it's more vigorous than a mature fruit.

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