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mcsuperd
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Registered: 10/24/14
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Last seen: 4 years, 1 month
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Panaeolus grains weird looking
#21337370 - 02/27/15 09:18 AM (9 years, 10 months ago) |
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I don't think there's contam. It's just there's two genetics that started from 2 inoc points that just won't cross. There's the lines of separation in the pics. Is it normal for pans? One genetic got light rhizo growth while the other does not.
 
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mycomattie


Registered: 11/15/12
Posts: 1,323
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Re: Panaeolus grains weird looking [Re: mcsuperd]
#21337473 - 02/27/15 09:46 AM (9 years, 10 months ago) |
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Wow - a lot of condensation; how wet is your substrate?
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cronicr



Registered: 08/07/11
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Re: Panaeolus grains weird looking [Re: mycomattie]
#21337537 - 02/27/15 10:01 AM (9 years, 10 months ago) |
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looks very bacterial
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I'm tired do me a favor
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mcsuperd
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Re: Panaeolus grains weird looking [Re: cronicr]
#21339515 - 02/27/15 05:35 PM (9 years, 10 months ago) |
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It's just half litter sub in a 1 litter jar, lots of sub and maybe too tight poly but I don't think there's bacteria, my cube jars had no bacteria, clean.
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PirateSwazey


Registered: 12/12/12
Posts: 2,993
Loc: Here, Now
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Re: Panaeolus grains weird looking [Re: cronicr]
#21341120 - 02/28/15 12:25 AM (9 years, 10 months ago) |
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Quote:
cronicr said: looks very bacterial
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,972
Loc: Canada
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Re: Panaeolus grains weird looking [Re: cronicr]
#21341129 - 02/28/15 12:28 AM (9 years, 10 months ago) |
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Quote:
cronicr said: looks very bacterial
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mcsuperd
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Re: Panaeolus grains weird looking [Re: Pastywhyte]
#21341589 - 02/28/15 05:31 AM (9 years, 10 months ago) |
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goddamn it guys it's clean. You think it got wet spots? It's not, the outter grains got sticky and after consolidating there's no more wet spots as the cake shrinks and grains stop sticking to the jar. My question is that is it normal for tomentose/rhizo pans genetics to not cross
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bw86
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Registered: 11/12/06
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Re: Panaeolus grains weird looking [Re: mcsuperd]
#21341681 - 02/28/15 06:29 AM (9 years, 10 months ago) |
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what filter do you have on there? how big of a hole for GE
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Pastywhyte
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Registered: 09/15/12
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Loc: Canada
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Re: Panaeolus grains weird looking [Re: bw86]
#21341761 - 02/28/15 07:15 AM (9 years, 10 months ago) |
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Pan don't typically have rhyzomorphic features. That big gap of really weak mycelium looks like colonized grains that are also contamed with bacillus. Lines of seperation between two isolates may occur on agar but, IME on 3D media like grain that does not happen. When you say two inoculation points with two genetics do you mean to say you inoculated with two isolates. Or two points of ms. Cause if ya used ms you have thousands of genetics in there. Those lines might be comprised of different species.
I would not use that jar.
Edited by Pastywhyte (02/28/15 07:23 AM)
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insanemike

Registered: 02/23/14
Posts: 4,272
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Re: Panaeolus grains weird looking [Re: Pastywhyte]
#21341901 - 02/28/15 08:28 AM (9 years, 10 months ago) |
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Op, does the room in which you keep your jars fluctuate in temperature? Could be the reason for the condensation on the jars. It happens to me all the time at night when the rooms temperature drops. The water begins to condensate on the glass because of the temp differential between the inside and outside of the jar. The jar does an excellent job of insulating the heat generated during the day and does not drop as fast as the temp in the room.
As long as the myc colonizes all the grain, go a head and spawn it to bulk. Just dont g2g because of the bacteria. Did you soak and boil or just boil those grain when prepping them? Bacteria endospores can take up to 8 hours to activate. Endospores are very heat and pressure resistant. Like I was informed the other day, sterilization in this hobby is a loose word for our "properly" sterilized grain prep. Although I do understand that complete sterilization is not optimum and not needed to get fine results. By the way I cook my quart jars for 2 hours at 15psi and don't ever have bacterial problems.
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bw86
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Re: Panaeolus grains weird looking [Re: bw86]
#21341923 - 02/28/15 08:34 AM (9 years, 10 months ago) |
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Quote:
bw86 said: what filter do you have on there? how big of a hole for GE
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mcsuperd
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Registered: 10/24/14
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Re: Panaeolus grains weird looking [Re: insanemike]
#21345674 - 02/28/15 08:04 PM (9 years, 10 months ago) |
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The filter hole is like 2mm in diameter
Quote:
Pastywhyte said: Pan don't typically have rhyzomorphic features. That big gap of really weak mycelium looks like colonized grains that are also contamed with bacillus. Lines of seperation between two isolates may occur on agar but, IME on 3D media like grain that does not happen. When you say two inoculation points with two genetics do you mean to say you inoculated with two isolates. Or two points of ms. Cause if ya used ms you have thousands of genetics in there. Those lines might be comprised of different species.
I would not use that jar. 
Two MS inoc points. The reason I say it's not bacteria that cause that because of the tomentose/rhizo feature of the myc that expands from the points. This jar will be spawned not g2g nevertheless.
Quote:
insanemike said: Op, does the room in which you keep your jars fluctuate in temperature? Could be the reason for the condensation on the jars. It happens to me all the time at night when the rooms temperature drops. The water begins to condensate on the glass because of the temp differential between the inside and outside of the jar. The jar does an excellent job of insulating the heat generated during the day and does not drop as fast as the temp in the room.
As long as the myc colonizes all the grain, go a head and spawn it to bulk. Just dont g2g because of the bacteria. Did you soak and boil or just boil those grain when prepping them? Bacteria endospores can take up to 8 hours to activate. Endospores are very heat and pressure resistant. Like I was informed the other day, sterilization in this hobby is a loose word for our "properly" sterilized grain prep. Although I do understand that complete sterilization is not optimum and not needed to get fine results. By the way I cook my quart jars for 2 hours at 15psi and don't ever have bacterial problems.
I keep it in a closet and I soak then boil then PC never had any contam in any jar except some wetspot
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Pastywhyte
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Registered: 09/15/12
Posts: 37,972
Loc: Canada
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Re: Panaeolus grains weird looking [Re: mcsuperd]
#21345696 - 02/28/15 08:09 PM (9 years, 10 months ago) |
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Quote:
because of the tomentose/rhizo feature of the myc that expands from the points
I have never seen rhyzomorphic pan myc IRL or online. I don't really see any in your photo either 
I still say that jar looks bacterial. After a second look I would like to add moldy to the diagnosis. Your not gonna change my mind. But its your grow and you gonna do what you want so I wish you well
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mcsuperd
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Re: Panaeolus grains weird looking [Re: Pastywhyte]
#21346729 - 03/01/15 02:41 AM (9 years, 10 months ago) |
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Yeah I'll post my results It's not solid rhizo like cube. I'll borrow my mom's phone to take another pic later lol
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mycomattie


Registered: 11/15/12
Posts: 1,323
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Re: Panaeolus grains weird looking [Re: mcsuperd]
#21347658 - 03/01/15 10:46 AM (9 years, 10 months ago) |
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Quote:
I have never seen rhyzomorphic pan myc IRL or online. I don't really see any in your photo either 
Same, I've never come across rhizomorphic Pan mycelium - not on agar, grains - nowhere.
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