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Offlinetourrat
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Registered: 01/16/14 Happy 11th Shroomiversary!
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Re: My last monotub failure - I don't know what I can improve on anymore....!? [Re: Chowder]
    #21345610 - 02/28/15 07:35 PM (9 years, 10 months ago)

I know a lot of people use cakes to spawn but I NEVER had any luck with it...always went green on me tried it maybe half dozen times....I think as nutritional as brf is,if the cakes aren't perfectly colonized ( tiny little uncolonized spot in middle) as soon as you crumble and expose that spot ...bam trich.  I'm no pro but that's all I could figure was happening when it happened ...I have WAY better luck with grains. Keep at it man...try your grains...just make sure they are fully colonized.


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Knee deep in the hotel tub.....

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OfflineChowder
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Re: My last monotub failure - I don't know what I can improve on anymore....!? [Re: tourrat]
    #21345626 - 02/28/15 07:43 PM (9 years, 10 months ago)

tourrat Thanks this could be it I guess. Though I think I did have some success with PF cakes in monotub before. I was in a different house, and I wonder if that's the issues. But from what I understand, the cleaness of the house is more important in the inoculation/culture procedures rather than in fruiting stages.


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InvisibleSoupSandwich
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Re: My last monotub failure - I don't know what I can improve on anymore....!? [Re: Chowder]
    #21345794 - 02/28/15 08:32 PM (9 years, 10 months ago)

How are you breaking up the cakes to put into the monotub?

Just curious...for my own personal knowledge.

Thanks,
SoupSandwich

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OfflineChowder
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Re: My last monotub failure - I don't know what I can improve on anymore....!? [Re: Chowder]
    #21345930 - 02/28/15 08:53 PM (9 years, 10 months ago)

Gloves and cheese grater, both cleaned with hot soapy water and wiped thoroughly with IPA.


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InvisibleSoupSandwich
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Re: My last monotub failure - I don't know what I can improve on anymore....!? [Re: Chowder]
    #21345967 - 02/28/15 09:01 PM (9 years, 10 months ago)

In a small room with air purifier? Are you wearing a mask?

I can't imagine just doing this out in the open. :frown:

But what do I know, never tried this procedure myself.

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InvisibleStipe-n CapMDiscord
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Re: My last monotub failure - I don't know what I can improve on anymore....!? [Re: Chowder]
    #21346038 - 02/28/15 09:14 PM (9 years, 10 months ago)

Quote:

Chowder said:
Gloves and cheese grater, both cleaned with hot soapy water and wiped thoroughly with IPA.





Stop doing this. If you insist on crumbling cakes to bulk just break it up in a ziplock freezer bag. I would move on to grains and stop beating a dead horse.

Ms to agar, agar to grains, grains to bulk. If you have the equipment available start using LI or even slurry your PF jars.

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InvisiblePastywhyteMDiscord
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Re: My last monotub failure - I don't know what I can improve on anymore....!? [Re: Stipe-n Cap] * 1
    #21346165 - 02/28/15 09:46 PM (9 years, 10 months ago)

Nothing wrong with a cheese grater. If the cake was contamed with trich then its gonna trich out. Spore syringes are not as clean as people like to think. Also breaking the cake up may have allowed the contam to get ahead and spread further but its not the methods fault. Dirty syringes are going to mess up your shit no matter what your substrate media is. OP is on the right track with agar tho, its a game changer :thumbup:

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Offlineshroomium
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Re: My last monotub failure - I don't know what I can improve on anymore....!? [Re: Pastywhyte]
    #21346194 - 02/28/15 09:55 PM (9 years, 10 months ago)

You have contaminants around somewhere. I think it is in the polyfill but I don't know for sure. Clean everything twice and buy a filter to tape to a box fan. Scrubbing the air will help. Get new poly or boil the stuff you have. make sure you are working in a room without unnecessary air flow(HVAC vents). Clean every surface with a 10% bleach solution, clean it twice! I also think that grains are a better option but I don't think that they would help in this instance.

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InvisiblePastywhyteMDiscord
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Re: My last monotub failure - I don't know what I can improve on anymore....!? [Re: shroomium] * 1
    #21346225 - 02/28/15 10:06 PM (9 years, 10 months ago)

Quote:

shroomium said:
You have contaminants around somewhere.




A cubic meter of air in your average house contains around 250000 contams.

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InvisibleStipe-n CapMDiscord
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Re: My last monotub failure - I don't know what I can improve on anymore....!? [Re: Pastywhyte]
    #21346415 - 02/28/15 11:21 PM (9 years, 10 months ago)

Ya I guess its no worse than using your hands, as long as the spawn is clean and fully colonized. My bad OP, you should really consider starting with agar.

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OfflineSockadin
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Re: My last monotub failure - I don't know what I can improve on anymore....!? [Re: Chowder]
    #21346728 - 03/01/15 02:40 AM (9 years, 10 months ago)

Quote:

Chowder said:


As you can see in my gallery (can you see actually?), my monotubs are just a series of contamination or bad pinsets with low yield.

This last one I thought it was perfect. I had clean consolidated PF cakes, crumbled using clean gloves. I had cleaned my tub with hot soapy water and wiped with IPA afterwards. For substrate I used Frank's proper pasteurization tek to the T.

The only thing I thought I might be doing wrong was the level of substrate moisture - something that cannot be conveyed accurately in instructions. I can only subjectively rely on the squeeze test.

I thought maybe my sub was too moist, so in this last tub that you can see above, I was really optimistic that making my sub drier was the thing I was missing. Still, fail!

Are there any extra-steps that I can take?  Does it help if I pasturize my sub for longer? Is it my coir to blame or is it the mould in my house to blame?

I'm chuffed to have done my first easy-agar tek from spores, and now I have 12/15 clean fully colonised rye jars - from agar wedges. Considering  the tens of jars I got wrong in the past, this is an overwhelming success. No way I'll ever inject spores in my rye again. (Also, I was very much looking towards doing clones on agar for the first time, from the fruits that I failed to get above.......)

But I'm so afraid of losing them to trich. again. I dont wanna see 3 beautiful monotubs ruined. No to mention all the coir/verm/gas wasted.

Where do you think I'm getting it wrong?
I dont think I've ever needed shroomery's advice as much as now.

At this point I'm tempted to go back to SGFC again, just to get fruits and my confidence back. But I dont have the time to spray/fan 3x/day anymore.





I know there are a lot of people who do the slurry and or crumble jars. Sounds like you got some good rye jars going. I would make step back and write down your entire clean procedure. Make a list of all of the subs you are using, and brand. Then look at these steps and look for the problem area.

If you are getting trich in your tubs it is usually because of dirty spawn. 99% of the time it is dirty spawn.

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OfflineChowder
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Re: My last monotub failure - I don't know what I can improve on anymore....!? [Re: Sockadin]
    #21346880 - 03/01/15 06:01 AM (9 years, 10 months ago)

Thank you all for the replies.

Basically, the only reason I still do cakes is because I failed so many times with rye. I was doing spores to rye, and I was getting a rainbow of contams. Amen to agar being a game-changer. And thanks Pastywhyte for the easy-agar tek.

I still have all the materials to do cakes, and the confidence of the procedure. I might still carry on for a while, but use a SGFC. Just for the fun/experience of it.


Cross-fingers with these 12 beautiful rye jars that I got.
4 quart jars + 7 quart of substrate / monotub should be good, right?
Right now, I just hope to get fruits > so I can clone > so I can CANOPY. I'd love to canopy!  :zombiedrool:

So jelly looking at people who go to monotubs at their first grow and get this right off the bat. Makes me feel like a complete idiot...


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InvisibleSoupSandwich
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Re: My last monotub failure - I don't know what I can improve on anymore....!? [Re: Chowder]
    #21347108 - 03/01/15 08:10 AM (9 years, 10 months ago)

That's weird because I never have any problems with multispore to straight rye. huh.

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InvisiblePastywhyteMDiscord
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Re: My last monotub failure - I don't know what I can improve on anymore....!? [Re: SoupSandwich]
    #21347133 - 03/01/15 08:20 AM (9 years, 10 months ago)

Quote:

SoupSandwich said:
That's weird because I never have any problems with multispore to straight rye. huh.




Thats cause ya ain't done it much. Run 100 monos and you might change your mind. Some people have better luck than others.  Whole reason I started agar was I couldn't get a clean syringe to save my life. Those vendors of course are no longer sponsors so good riddence. But even the best vendors still put out a dirty syringe occasionally.

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InvisibleSoupSandwich
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Re: My last monotub failure - I don't know what I can improve on anymore....!? [Re: Pastywhyte]
    #21347146 - 03/01/15 08:25 AM (9 years, 10 months ago)

I haven't done 100, maybe 50, throughout my entire life, I'd say something like less than 5% have contaminated.

Monos seem like they'd be much more contam prone to me, but what do I know?

I was merely suggesting that maybe, just maybe, there's a problem with his sterile technique.

Maybe.



Maybe.

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OfflineFreeWorldOrder
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Re: My last monotub failure - I don't know what I can improve on anymore....!? [Re: Pastywhyte]
    #21347188 - 03/01/15 08:38 AM (9 years, 10 months ago)

Quote:

Pastywhyte said:
Nothing wrong with a cheese grater. If the cake was contamed with trich then its gonna trich out. Spore syringes are not as clean as people like to think. Also breaking the cake up may have allowed the contam to get ahead and spread further but its not the methods fault. Dirty syringes are going to mess up your shit no matter what your substrate media is. OP is on the right track with agar tho, its a game changer :thumbup:





Exactly. I've used a rusty old cheese grater time and again and works fine.


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