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OfflineDnuobe
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Registered: 12/10/05
Posts: 15
Last seen: 18 years, 11 months
Little over 4 weeks incubating
    #5185000 - 01/15/06 08:08 PM (19 years, 5 days ago)

It's been a little over 4 weeks now since I inoculated my 5X12 racks of jars with 5 different strands. The mycelium has definitely come along but it seems to be slowing down a lot lately.

My main concern is that I'm not incubating at high enough temperatures. I've moved my racks around trying to find the perfect incubating area, and have now settled for them stacked in a corner of my closet with a towel surrounding them. With them I have a light going (the racks are in garbage bags to keep out light.

The last week the temperatures have been going to around 70 to 75 degrees and I'm trying to get them back up to around 85. Is the temperature more than likely the reason they're not done yet? All of them seem to be perfectly hydrated still and they grow, but seem to have slowed drastically over the week since I've been back at my apartment.

Most of them are over 2/3 done with a few covered, but they seem to have slowed down a lot.

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Offlinelemon_lw
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Registered: 10/17/04
Posts: 3,622
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Re: Little over 4 weeks incubating [Re: Dnuobe]
    #5185017 - 01/15/06 08:12 PM (19 years, 5 days ago)

yes your temp is the main culprit, try making a good incubating chamber for future grows, its money well spent. but with your time you MIGHT need to do a forced air exchange or flipping.


--------------------
In the belly of the Leviathan, one can either despair and perish, or be cheerful and persevere.-Dean Koontz

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OfflineDnuobe
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Registered: 12/10/05
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Last seen: 18 years, 11 months
Re: Little over 4 weeks incubating [Re: lemon_lw]
    #5185042 - 01/15/06 08:17 PM (19 years, 5 days ago)

Quote:

lemon_lw said:
yes your temp is the main culprit, try making a good incubating chamber for future grows, its money well spent. but with your time you MIGHT need to do a forced air exchange or flipping.




I have two holes and tin foil over the jars, should I just flip them over and leave them or do I have to do some process to exchange? I'm going to check what the new temp in my new little area is and then if it's good I'm going to flip them.

Also generally the cakes are ready 2-3 days after the jar is covered right? Giving it time to consume the middle?

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Offlinedroptopsunfire03
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Registered: 08/04/05
Posts: 174
Last seen: 17 years, 1 month
Re: Little over 4 weeks incubating [Re: Dnuobe]
    #5185060 - 01/15/06 08:21 PM (19 years, 5 days ago)

Take the foil off. Air cant get through very good with the holes covered, id guess poor air exchange would be the culprit, 4 weks is a long time, i usually only put my cakes in room temp and 3 weeks is the very longest theyve ever taken. Id suggest taping a small patch of polyfill over the hole tho to try and help keep contams out, that way you wont have to open the jar to use tyvek.

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Offlinecoda
Banjo Goiter
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Registered: 03/20/01
Posts: 8,750
Last seen: 1 year, 9 months
Trusted Cultivator
Re: Little over 4 weeks incubating [Re: droptopsunfire03]
    #5185066 - 01/15/06 08:24 PM (19 years, 5 days ago)

Tinfoil restricts the gas exchange, if you're using PF tek the dry verm barrier is there to protect you against contams. Remove tinfoil, flip jars, and try to raise your temps into the 80's.


--------------------
To get really high is to forget yourself. And to forget yourself is to see everything else. And to see everything else is to become an understanding molecule in evolution, a conscious tool of the universe. And I think every human being should be a conscious tool of the universe. . . .

-JG

i really am glad you came back to us instead of taking the other path. *hug*

-A_S (RIP your final words to me will never be forgotten)



Don't fuck with the laughing jesus.

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Offlinelemon_lw
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Re: Little over 4 weeks incubating [Re: Dnuobe]
    #5185076 - 01/15/06 08:26 PM (19 years, 5 days ago)

to do a frce exchange take your jar over a hepa filter and let the air blow into it. if you flip make sure to tap the bottom of the jar so that the substrate isnt touching it. and yes 2-3 days is the time to wait. and once again a better incubator, what are your plans for a fruiting chamber?


--------------------
In the belly of the Leviathan, one can either despair and perish, or be cheerful and persevere.-Dean Koontz

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OfflineDnuobe
Stranger
Registered: 12/10/05
Posts: 15
Last seen: 18 years, 11 months
Re: Little over 4 weeks incubating [Re: lemon_lw]
    #5186319 - 01/16/06 08:29 AM (19 years, 5 days ago)

Quote:

lemon_lw said:
to do a frce exchange take your jar over a hepa filter and let the air blow into it. if you flip make sure to tap the bottom of the jar so that the substrate isnt touching it. and yes 2-3 days is the time to wait. and once again a better incubator, what are your plans for a fruiting chamber?




I still have to go out and get a couple plastic containers (smaller heightwise than a rubbermaid) and then I'm going to drill holes in the sides towards the bottom. For the top I'll probably get a plastic bag or something to put over it. Then I'll have a lamp shining over the top.

For now though I'm going to flip them over, thx for the tip guys!

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InvisibleTaliesin
Realm Walker
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Registered: 04/14/14
Posts: 100
Re: Little over 4 weeks incubating [Re: lemon_lw]
    #21342481 - 02/28/15 10:34 AM (9 years, 10 months ago)

I understand that this thread is really old. But id like to state for anyone who is reading this that found it by looking for answers to your questions... An Incubator is NOT a good idea. The lid on it creates an enclosed micro climate caused by condensation from the heated water in the bottom reservoir. Plus with the lid on the incubator, it will eventually stall GE and give way to all sorts of issues. Its best to find a shelf in a room that stays anywhere between 68(at night) and 75 IMO. The heat caused by the Myc. colonizing the substrate can raise the internal temperature by up to(in some cases) ten degrees. The saying that the hotter the temp the faster the colonization is a Myth and was formulated through trial with Agar on petri(sp) dishes that didn't hold heat.
  Anything over 80 degrees will increase your chances of contamination. Speaking from experience, Bacteria seems to thrive in the bottom of well heated incubators and on the inside of over heated jars. I used a incubator for a long time and just recently stopped because of the high amount of jars I've lost up until that point. My tub always started smelling like sweaty gym socks.
  Dont do it folks. A shelf in normal room temperature is all you need. If you're comfortable in a shirt, then your Myc is comfy too. Also don't fret about contamination from the air. Just follow normal cleaning procedures in the immediate area and use a well reputable filter system on your jars. Chances are high that if you experience contamination while they're on the shelf, its because of improper sterilization procedures during Jar sterilization and/or inoculation. Work in a glove box in a small draft-less room that you've cleaned. Always take a shower before and wear clean, freshly laundered clothing.
As far as jar filters, I'm using poly-fill at the moment, but my next round will have newly fashioned Tyvek filter lids with silicone injection ports. Ill be upgrading the filters from Tyvek to SFD's as I found a lot of 12 wide mouths filters on ebay for ten bucks. I plan on cutting them into smaller circles to cover the 3/8 or 1/4 GE holes on my lids, fastened securely with 100% silicone that's made to withstand temps of up to 400.
I humbly share this information with anyone who may read this and recommend that when in doubt, keep reading. There is a lot of old outdated information still floating around. I would like to spare any of you from my own mistakes in the past.

Peace n' Love


--------------------
"That which you manifest is before you"

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