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mushpunx
Fungus Punk



Registered: 04/20/14
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Cloning question
#21340220 - 02/27/15 08:39 PM (9 years, 2 months ago) |
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When transfering a clone again and again to clean it up do you cut out genetics to the point that it usually matters?
Likke unless youre trying to isolate sectors on purpose would just cleaming or expanding a clone over time change the clone?
Thanks!
-mush
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uncle_rico
my own worst enemy


Registered: 03/28/06
Posts: 2,664
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Re: Cloning question [Re: mushpunx]
#21340311 - 02/27/15 08:58 PM (9 years, 2 months ago) |
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Most of the tissue cultures I have worked with, have 2 to 4 'strains' working in the culture.
When you clean up your culture (taking clean, leading edge growth), you are isolating the cultures.
When you get the culture cleaned-up, grow it out. You may have isolated it to a mono-culture. already
 here is a clone culture (same culture both sides of the petri dish). How many separate cultures do you see? three?
many growers prefer to stay with a clone grow (2 to 4 cultures) for the diversity / flexibility. good luck
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NumeroEno
I come from the land of lizards



Registered: 07/24/14
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Also I'd imagine that if you take transfers before it starts sectoring you'll keep the strains together.
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mushpunx
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Well like for example lets say you clone a bad asd cluster, and through cleaning it up you accidentially isolate it down to a monoculture... wouldnt it not grow out in clusters anymore?
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taGyo
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Re: Cloning question [Re: mushpunx]
#21340501 - 02/27/15 09:35 PM (9 years, 2 months ago) |
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Every time you cut away from your mycelium you're excluding genetics. Some of those genetics could be powerful fruiting potential. That's why I'd rather have a clone then an isolate. An isolate is very good for testing things and repeated performance BUT you could get to that 12th transfer and be fucked in fruiting potential, where it matters most.
IMO if you're going to a monoculture get a good clone, transfer to petri 2 and fruit petri 1. Then transfer to petri 3 and fruit petri 2. If petri 1 outperforms Petri 2 and 3 then you isolated away.
Maybe I'm looking at it backwards. I would just hate to isolate and isolate and then get three mushrooms when I could have taken a clone and gotten ounces.
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SpitballJedi
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Re: Cloning question [Re: taGyo]
#21340612 - 02/27/15 10:02 PM (9 years, 2 months ago) |
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I've cloned MS fruits and sectored them with shitty results before. Some genetics may be working together to create that nice fruit you want to clone. Then, when you separate those genetics, you can get different results.
This is one reason some people like to get a bunch of isolates from MS and grow them out separately. Keep the good isolates and toss the bad ones. Some find this to be a more efficient process than cloning then isolating to get the results you want.
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mushpunx
Fungus Punk



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Re: Cloning question [Re: taGyo]
#21340625 - 02/27/15 10:07 PM (9 years, 2 months ago) |
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Right on Tagyo
Yea man I run clones most of the time. In my spare time Ive been learning to isolate out momo cultures all the way from spores and Isolating the different sectors from clones and testing them out and whatnot.
But for my main spawn runs I mostly run clones.
Im just wondering like if I have a clone cluster I really wanna keep intact will it lose enough genetics just from cleaning it up or moving it around that it would cease to be a clister?
Like if the genetics work together nicely Im worried I might accentially split them up
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taGyo
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Quote:
SpitballJedi said: I've cloned MS fruits and sectored them with shitty results before. Some genetics may be working together to create that nice fruit you want to clone. Then, when you separate those genetics, you can get different results.

Perfect explanation. I agree monos have their place, I just hate transferring a million times for a guess 
You get my message mush?
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SpitballJedi
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Re: Cloning question [Re: mushpunx]
#21340639 - 02/27/15 10:09 PM (9 years, 2 months ago) |
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sometimes, the best answers come from your own experiments.
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taGyo
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Once again. I just prefer clones. One day I'm sure I'll have a bunch of monos with great potential but meh.
All of your plates look beautiful btw Mush, I was like o_o
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mushpunx
Fungus Punk



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Quote:
SpitballJedi said: sometimes, the best answers come from your own experiments. 
Hahaha right on right on.
I get a question in my head sometimes when Im working and itll drive me nuts if I dont at least ask haha Trouble is I cant verbalize it right haha
Thanks guys!
Tagyo - thanks dude! Oh btw I got your message, hell yea!! Thanks
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taGyo
Strainiac/AMU



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Re: Cloning question [Re: mushpunx]
#21340668 - 02/27/15 10:20 PM (9 years, 2 months ago) |
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SpitballJedi
Ancient Astronaut



Registered: 10/13/12
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Re: Cloning question [Re: taGyo]
#21340837 - 02/27/15 11:02 PM (9 years, 2 months ago) |
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I prefer clones too.
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Psilicon
Really Nice Guy


Registered: 08/26/12
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So do I, and I'm also one of those who prefers isolates taken from clones than those isolated straight from agar. At least with a clonal isolate you know that at some point there was at least one set of genetics in the bunch that wanted to fruit. You don't get that kind of guarantee with an isolate from MS.
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SpitballJedi
Ancient Astronaut



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Re: Cloning question [Re: Psilicon]
#21340867 - 02/27/15 11:07 PM (9 years, 2 months ago) |
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eutebius



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Thanks, guys, for this discussion. I've stuck with isolating from clones, and have been wondering if I'm missing out on better grows...
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SpitballJedi
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Re: Cloning question [Re: eutebius]
#21342463 - 02/28/15 10:30 AM (9 years, 2 months ago) |
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Probably not given this is a hobby. If you were trying to run a commercial farm I would say follow the spore-> isolate route.
You can do both. Keep your clone grows like you already do and start working on some spore isolates. You can test them with smaller grows like minimonos and bottle tek. With bottle tek, you can test several isolates at once in the same FC
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