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OfflinesomethingIchy
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Registered: 12/11/14
Posts: 13
Last seen: 8 years, 11 months
first post. 100th contam
    #21340032 - 02/27/15 07:53 PM (9 years, 10 months ago)

first things first. hello errybody!

so some history. I've tried my hands at a few different methods, and thus far I've got contaminates nearly every time.

tried the bag tek with LC and got a grip of contams.

i also tried using the LC in WBS in jars, and again it was contam-city in all the jars...

so i took a few samples from what grew out, and made a few agar plates of them, then used those to actually get a crop out using the wbs in jars, but even then I've only had a very few jars go with out getting contaminated.

im now trying my hand at the BRF tek and im starting to see what i feel are bad signs from the start.

I've built a glove box that gets doused in Lysol or all purpose cleaner with bleach (depending on how i feel at the time) before anything gets opened in it.

I flame sterilize my needles,
i wear a face mask and gloves,
the birdseed and BRF cakes get 15-17psi for at least 90min every time.

and yet these are the results I come up with every time.















i did my best to get the clearest shots o could but the glare is a b*tc# to avoid without any of my filters available.

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InvisibleSpitballJedi
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Registered: 10/13/12
Posts: 8,598
Loc: Nibiru Flag
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Re: first post. 100th contam [Re: somethingIchy]
    #21340092 - 02/27/15 08:08 PM (9 years, 10 months ago)

I know it can be frustrating and I went through the same shit when I first started. At one point. I just said fuck it and walked away.

don't use a glove-box. Use a SAB instead. It's better for this hobby.

Putting contamianted myc from a grow canbe tricky to cleanup because the contam and the healthy myc are blended in together.

Instead, put your spores on the agar in your SAB. Once you get a little growth, transfer a small pice from the outer edge of the healthiest myc to a new agar plate in your SAB. Take a few samples and make a few new plates to hedge your bets.

Once those grow out a little, transfer again using the healthiest looking growth. This is how you clean up your spores and make a clean culture. Once you get an agar plate with clean myc, use it to inoculate your jars.

You may end up with several plates that you can store for later or just toss. This take s a little more time, but it's the best chance of success.

You Should Really Start With Agar


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Offlinesilverstem
Caps & Stems
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Registered: 10/12/13
Posts: 900
Loc: jordan Flag
Last seen: 7 years, 5 months
Re: first post. 100th contam [Re: SpitballJedi]
    #21340368 - 02/27/15 09:10 PM (9 years, 10 months ago)

Grab a fan point it out a window grab another fan point it into ur house.. Let ur room get aired out for 4 hours... Also did u grind up ur wbs when u did pf tek? Are you sure ur syringes sent contaminated?


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OfflinetaGyo
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Registered: 10/16/14
Posts: 18,802
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Re: first post. 100th contam [Re: SpitballJedi]
    #21340534 - 02/27/15 09:41 PM (9 years, 10 months ago)

Quote:

SpitballJedi said:
don't use a glove-box. Use a SAB instead. It's better for this hobby.

You Should Really Start With Agar




Really, it sounds like you got bad spores which means it's in no way indicative of YOUR skills as a mycologist.

I really recommend starting with agar.


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OfflineTrrrex92
Just here.
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Registered: 12/05/13
Posts: 488
Last seen: 3 years, 9 months
Re: first post. 100th contam [Re: taGyo]
    #21340550 - 02/27/15 09:45 PM (9 years, 10 months ago)

It sounds like the LC you used could have been contaminated. If everything you tried to colonize with it ended up contaminated.

And the Brf jars looks like weak mycelium to me. Bad genetics maybe?

Edited by Trrrex92 (02/27/15 09:47 PM)

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InvisiblePirateSwazey
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Registered: 12/12/12
Posts: 2,993
Loc: Here, Now
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Re: first post. 100th contam [Re: Trrrex92]
    #21340830 - 02/27/15 11:02 PM (9 years, 10 months ago)

Take it back to agar & grain and step away from the liquid inoculants & BRF.

This will surely bring you better results... stay thirsty my friend!


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