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Ghatti
Totally not a Federal Agent

Registered: 06/23/14
Posts: 1,733
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Re: Continuing Contam issue [Re: Pastywhyte]
#21342278 - 02/28/15 09:49 AM (9 years, 10 months ago) |
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Since switching to bags, no.
I proposed a plan to isolate and rule things out above but didn't receive any feedback.
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Ghatti
Totally not a Federal Agent

Registered: 06/23/14
Posts: 1,733
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Re: Continuing Contam issue [Re: Ghatti]
#21342288 - 02/28/15 09:51 AM (9 years, 10 months ago) |
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Quote:
Ghatti said: To isolate the issue I'm going to pc 2 bags. One I will false inoculate and tie up and the other I will immediately seal when I pull out of the pc. If neither contaminate then it is how/what I am making my LI with. If the false innoc one contams then my hood/technique is at fault, if both contaminate then my pc time is not long enough.
Does this sound correct?
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taGyo
Strainiac/AMU



Registered: 10/16/14
Posts: 18,802
Loc: Journal Land
Last seen: 6 years, 3 months
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Re: Continuing Contam issue [Re: Pastywhyte]
#21342295 - 02/28/15 09:52 AM (9 years, 10 months ago) |
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Quote:
Pastywhyte said:
Have you left some grain uninoculated for the same time your grains take to colonize? That will be the only way to rule that out.
Sterilize some bags and leave them out for a spawn run. Don't inoculate them. If they contaminate before 2-3 weeks then you're not PCing long enough. Pasty answered that.
-------------------- Gyo's Better Grows
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Dominus fortunae meae sum
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tripdawg420
low life with no life



Registered: 02/02/09
Posts: 7,653
Loc: illinois
Last seen: 1 hour, 19 minutes
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Re: Continuing Contam issue [Re: taGyo]
#21342336 - 02/28/15 10:00 AM (9 years, 10 months ago) |
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bag are differant than jars you really cant leave them out they will start sucking in air thur the top of the bag
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Ghatti
Totally not a Federal Agent

Registered: 06/23/14
Posts: 1,733
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Re: Continuing Contam issue [Re: tripdawg420]
#21342472 - 02/28/15 10:32 AM (9 years, 10 months ago) |
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Lol I'm sure he meant to seal it.
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taGyo
Strainiac/AMU



Registered: 10/16/14
Posts: 18,802
Loc: Journal Land
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Re: Continuing Contam issue [Re: Ghatti]
#21342493 - 02/28/15 10:36 AM (9 years, 10 months ago) |
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Of course I did 
I think he's saying even if it's sealed it will leak in.
BW does that look like good sterilization times to you? I know you do a lot of bags too.
-------------------- Gyo's Better Grows
TNF Q&A
AMU Q&A
Dominus fortunae meae sum
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Ghatti
Totally not a Federal Agent

Registered: 06/23/14
Posts: 1,733
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Re: Continuing Contam issue [Re: taGyo]
#21342506 - 02/28/15 10:38 AM (9 years, 10 months ago) |
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Since when will sealed bags leak?
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taGyo
Strainiac/AMU



Registered: 10/16/14
Posts: 18,802
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Re: Continuing Contam issue [Re: Ghatti]
#21342512 - 02/28/15 10:39 AM (9 years, 10 months ago) |
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Ask trip,
I don't use bags. I think it's in your spawn honestly.
-------------------- Gyo's Better Grows
TNF Q&A
AMU Q&A
Dominus fortunae meae sum
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Ghatti
Totally not a Federal Agent

Registered: 06/23/14
Posts: 1,733
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Re: Continuing Contam issue [Re: taGyo]
#21342519 - 02/28/15 10:41 AM (9 years, 10 months ago) |
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I'm 100% positive its my spawn. The question is if its incomplete sterilization, dirty culture, or faulty hood work.
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tripdawg420
low life with no life



Registered: 02/02/09
Posts: 7,653
Loc: illinois
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Re: Continuing Contam issue [Re: Ghatti]
#21342522 - 02/28/15 10:42 AM (9 years, 10 months ago) |
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lol 3.5 hours is well enought pc time i would say its your li but i use grain spawn to innoc grain bags might wana try that are you washing the jar down
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Ghatti
Totally not a Federal Agent

Registered: 06/23/14
Posts: 1,733
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Re: Continuing Contam issue [Re: tripdawg420]
#21342550 - 02/28/15 10:46 AM (9 years, 10 months ago) |
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As in after I make the LI, yes.
Also seem to have the one flush issue when just using wedges as well. I thought maybe PC time since the contam seems to take its time manifesting. Maybe I juuussstttt about killed it and it takes that long to recover and present itself.
I guess the only way to be certain is run the control test I stated.
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tripdawg420
low life with no life



Registered: 02/02/09
Posts: 7,653
Loc: illinois
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Re: Continuing Contam issue [Re: Ghatti]
#21342557 - 02/28/15 10:48 AM (9 years, 10 months ago) |
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,972
Loc: Canada
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Re: Continuing Contam issue [Re: tripdawg420]
#21342566 - 02/28/15 10:50 AM (9 years, 10 months ago) |
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Post a pic of what you would consider a clean plate and clean spawn. Do a jar run and leave some uninoculated. Don't even open them. See what they look like in a few weeks.
I would also suggest you run some muda bottles. They can teach a lot about your grain prep. Even if you don't fruit them you can learn things.
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taGyo
Strainiac/AMU



Registered: 10/16/14
Posts: 18,802
Loc: Journal Land
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Re: Continuing Contam issue [Re: tripdawg420]
#21342568 - 02/28/15 10:51 AM (9 years, 10 months ago) |
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And to show us pics of your next plate before you LI it.
EDIT:

Some molds are tricky.
-------------------- Gyo's Better Grows
TNF Q&A
AMU Q&A
Dominus fortunae meae sum
Edited by taGyo (02/28/15 10:51 AM)
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Ghatti
Totally not a Federal Agent

Registered: 06/23/14
Posts: 1,733
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Re: Continuing Contam issue [Re: taGyo]
#21342582 - 02/28/15 10:54 AM (9 years, 10 months ago) |
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I will leave a bag to sit for a few weeks and post a pic of the next plate I plan to use. Prolly be a week or more.
I'm seriously tired of throwing shit out. Its nice to at least get a flush but I wanna strangle every noob on their 5th flush lol.
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tripdawg420
low life with no life



Registered: 02/02/09
Posts: 7,653
Loc: illinois
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Re: Continuing Contam issue [Re: Ghatti]
#21342589 - 02/28/15 10:55 AM (9 years, 10 months ago) |
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,972
Loc: Canada
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Re: Continuing Contam issue [Re: tripdawg420]
#21342646 - 02/28/15 11:08 AM (9 years, 10 months ago) |
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Keep in mind that some cultures may only be good for one flush. I have a culture that is only good for one or two at most but I get 150% BE with it so its worth it. If I get two dry oz or more per quart of spawn used first flush, I really don't care if I see a second.
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hamloaf
Q-dood ®©™√


Registered: 12/23/09
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Re: Continuing Contam issue [Re: Ghatti]
#21342654 - 02/28/15 11:10 AM (9 years, 10 months ago) |
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Op, your issue is cross-contamination in your procedures of the step of inoculating of your sterile grain mediums. If your issue was dirty inoculm, and, or your sterilizer wasn't sterilizing properly, contamination would show up within 2-3 days.
IF you feel like your sterilizer isn't sterilizing properly, you can order indicator test strips that you run through the sterilizer with your grainmasters. The test strips won't tell you how long the 15psi was sustained for, but they will indicate if 15 psi is even being achieved by your PC.
Edited by liquidmyce (02/28/15 11:23 AM)
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tripdawg420
low life with no life



Registered: 02/02/09
Posts: 7,653
Loc: illinois
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Re: Continuing Contam issue [Re: Pastywhyte]
#21342659 - 02/28/15 11:12 AM (9 years, 10 months ago) |
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go from agar to grain master to spawn bag i wouldnt mess with any thing in between
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tripdawg420
low life with no life



Registered: 02/02/09
Posts: 7,653
Loc: illinois
Last seen: 1 hour, 19 minutes
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Re: Continuing Contam issue [Re: hamloaf]
#21342667 - 02/28/15 11:14 AM (9 years, 10 months ago) |
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Edited by tripdawg420 (02/28/15 11:20 AM)
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