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qwobbler
The Hunter


Registered: 01/14/15
Posts: 177
Loc: Tropics
Last seen: 7 years, 18 days
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Sterile and Sanitized Areas - Do I have the right stuff?
#21330003 - 02/25/15 07:31 PM (9 years, 10 months ago) |
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hey everyone, wanted to ask a question about my sterile procedure.
I use a SAB that i made.
I wear tyvek sleeves.
Always wear non powder coated Ansell disposable gloves.
I use Glen 20 (which is just like Oust) or a homebrand one that says it only contains 60% ethanol, to give a quick spray in the SAB and let settle for a few minutes before entering.
I've been using Isocol (http://www.isocol.com.au/product/isocol-rubbing-alcohol-antiseptic/) as its the only cheap thing i can find. I usually wet down paper towels with this stuff and lay them on the bottom of my SAB.
I use that clear hand sanitizer that apparently kills 99.99% germs and its evaporates quickly, to clean my hands then gloves.
I use a flame to sterilize my scalpel after everyone 1 cut i do.
Finally, because its so expensive where i live and hard to get, i use Diggers 100% isopropyl on a paper towel to finally wipe hands and jars down while inside the SAB before opening any agar plates or jars.
Isopropyl only comes in little bottles and doesnt last long. what else could i use? is the Isocol i mentioned above ok to use for everything?
Any suggestions on other products i can use to keep sterile?
Cheers
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Big Bear
Earf Child



Registered: 06/11/14
Posts: 5,417
Loc: In love, On time
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Re: Sterile and Sanitized Areas - Do I have the right stuff? [Re: qwobbler]
#21330016 - 02/25/15 07:33 PM (9 years, 10 months ago) |
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Idk if Lysol wipes are available to you, but I know some pretty damn good growers who use them.
-------------------- Need help growing? Ask AMU for hassle free answers.
Every year is getting shorter, never seem to find the time...
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mushpunx
Fungus Punk



Registered: 04/20/14
Posts: 13,394
Last seen: 2 months, 8 days
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Re: Sterile and Sanitized Areas - Do I have the right stuff? [Re: Big Bear]
#21330300 - 02/25/15 08:30 PM (9 years, 10 months ago) |
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Ive heard 70% is better than 100% Iso for sanitizing anyways, if you are using Isocol to wipe down the SAB you can use that in place of the Diggers. Rubbing alchol is easily available to you yea? Its just the 100% thats hard to find?
Actually just a spay/wipe down with dish soapy water is good for the SAB, I never use oust or lysol or anything except a quick shot of lysol on my flow hood before I turn it on.
Everything youre doing sounds good otherwise!
Edited by mushpunx (02/25/15 08:36 PM)
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sooperdooper
lazy fuck



Registered: 09/06/12
Posts: 1,253
Loc: This one place
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Re: Sterile and Sanitized Areas - Do I have the right stuff? [Re: mushpunx]
#21331338 - 02/26/15 02:11 AM (9 years, 10 months ago) |
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soapy water is fine for the sab. Really all you need is something to "grab ahold" of shit floating in the SAB.
Honestly ive gotten away with just tap water in a spray bottle & smooth hand movements 
ur fine man...good luck
-------------------- --------------------------------------------------------------------
99.9% of what i say is a lie...the other, gibberish.
    
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qwobbler
The Hunter


Registered: 01/14/15
Posts: 177
Loc: Tropics
Last seen: 7 years, 18 days
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Re: Sterile and Sanitized Areas - Do I have the right stuff? [Re: sooperdooper]
#21331397 - 02/26/15 02:57 AM (9 years, 10 months ago) |
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cheers for the input guys
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